Difference between revisions of "Team:NKU CHINA"
| (28 intermediate revisions by 2 users not shown) | |||
| Line 134: | Line 134: | ||
} | } | ||
.link2to{ | .link2to{ | ||
| − | |||
transform: translateX(-50%); | transform: translateX(-50%); | ||
-moz-transform: translateX(-50%); | -moz-transform: translateX(-50%); | ||
| Line 160: | Line 159: | ||
var bgVideo=document.getElementById("videohome"); | var bgVideo=document.getElementById("videohome"); | ||
bgVideo.addEventListener('canplaythrough',function(){ | bgVideo.addEventListener('canplaythrough',function(){ | ||
| − | + | $('#loading').css("display","none"); | |
| − | $('#loading'). | + | $('.loader-ring').css("display","none"); |
| − | $('.loader-ring'). | + | $('#loading_p').css("display","none"); |
| − | $('#loading_p'). | + | |
$('#PopQC_largr').css("display","block"); | $('#PopQC_largr').css("display","block"); | ||
$('#jun').fadeIn(4000); | $('#jun').fadeIn(4000); | ||
$('#sihuansu').fadeIn(4000); | $('#sihuansu').fadeIn(4000); | ||
| − | $('#pop_circle').css("display","block" | + | $('#pop_circle').css("display","block"); |
}); | }); | ||
}); | }); | ||
| Line 198: | Line 196: | ||
</div> | </div> | ||
<main> | <main> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/9/98/T--NKU_CHINA--PopQC_larger.gif" id="PopQC_largr" style="position:absolute; | + | <img src="https://static.igem.org/mediawiki/2018/9/98/T--NKU_CHINA--PopQC_larger.gif" id="PopQC_largr" style="position:absolute;left: 28%;top: -14%;z-index: 10;width: 600px;height: auto;"> |
| − | <img src="https://static.igem.org/mediawiki/2018/0/0e/T--NKU_CHINA--pop_circle.gif" id="pop_circle" style="position: absolute; | + | <img src="https://static.igem.org/mediawiki/2018/0/0e/T--NKU_CHINA--pop_circle.gif" id="pop_circle" style="position: absolute;left:34%;top: -3.5%;z-index: 10;"> |
| − | <img src="https://static.igem.org/mediawiki/2018/9/9d/T--NKU_CHINA--junfinal.png" id="jun" style="position: absolute; | + | <img src="https://static.igem.org/mediawiki/2018/9/9d/T--NKU_CHINA--junfinal.png" id="jun" style="position: absolute;top: 7.5%;left: 20%;width: 450px;height: auto;z-index:20;"> |
| − | <img src="https://static.igem.org/mediawiki/2018/f/f9/T--NKU_CHINA--sihuansu.png" id="sihuansu" style="position: absolute; | + | <img src="https://static.igem.org/mediawiki/2018/f/f9/T--NKU_CHINA--sihuansu.png" id="sihuansu" style="position: absolute;top: 4.5%;left: 55%;width: 500px;height: auto;"> |
<div class="container-fluid"> | <div class="container-fluid"> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
<img src="https://static.igem.org/mediawiki/2018/f/fb/T--NKU_CHINA--empty.png" class="img-responsive center-block"> | <img src="https://static.igem.org/mediawiki/2018/f/fb/T--NKU_CHINA--empty.png" class="img-responsive center-block"> | ||
| − | + | <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/b/bf/T--NKU_CHINA--arrowdown.gif" style="width: 100px;height: auto;cursor:pointer;z-index:1000;position:absolute;top:20%;left:48%;" onclick="scrollupone()"> | |
| − | < | + | |
| − | + | ||
<div class="container"> | <div class="container"> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <p | + | <p><br><br></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 219: | Line 216: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | <h2 style="color: white; font-size: | + | <h2 style="color: white; font-size: 60px; font-family: myTitle3;text-align: center;margin-top:30px;margin-bottom:20px;padding-top:0;padding-bottom:0;" id="dinwei">Short Video Display</h2> |
</div> | </div> | ||
| − | <div class="col-xs- | + | <div class="col-xs-12 col-xs-push-1"> |
<video preload="preload" controls="controls" width="800" height="auto" id="nku_video" poster="https://static.igem.org/mediawiki/2018/b/b2/T--NKU_CHINA--video_image.jpg"> | <video preload="preload" controls="controls" width="800" height="auto" id="nku_video" poster="https://static.igem.org/mediawiki/2018/b/b2/T--NKU_CHINA--video_image.jpg"> | ||
<source src="https://static.igem.org/mediawiki/2018/b/b6/T--NKU_CHINA--igem_NKU.mp4" type="video/mp4"> | <source src="https://static.igem.org/mediawiki/2018/b/b6/T--NKU_CHINA--igem_NKU.mp4" type="video/mp4"> | ||
| Line 227: | Line 224: | ||
</div> | </div> | ||
<div class="col-xs-12" style="margin-top: 10px;"> | <div class="col-xs-12" style="margin-top: 10px;"> | ||
| − | <p style="text-align: center; | + | <p style="text-align: center;"><a href="https://youtu.be/Gmr6zBYlpK0" style="color: white;font-size: 25px;">Cilck to watch our video on <span style="color: yellow;">YouTube</span></a></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 234: | Line 231: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-12"> | <div class="col-xs-12"> | ||
| − | + | <img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/b/bf/T--NKU_CHINA--arrowdown.gif" style="width: 100px;height: auto;cursor:pointer;" onclick="scrolluptwo()"> | |
| − | < | + | |
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 260: | Line 255: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-12" style="margin-top: 20px;"> | <div class="col-xs-12" style="margin-top: 20px;"> | ||
| − | <p style="color: white;font-size: 23px;line-height: 30px;text-align: justify;">Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in <i>Bacillus amyloliquefaciens</i> LL3 | + | <p style="color: white;font-size: 23px;line-height: 30px;text-align: justify;">Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in <i>Bacillus amyloliquefaciens</i> LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— γ-PGA.</p> |
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 00:32, 18 October 2018
Loading...
Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.
Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in Bacillus amyloliquefaciens LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— γ-PGA.