Difference between revisions of "Team:NKU CHINA"

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{{NKU_CHINA}}
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{{Template:NKU_CHINA/header}}
 
<html>
 
<html>
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<head>
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<style type="text/css">
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.footer-w3ls ul.social-icons1 li a {
 +
  margin-right:2px!important;
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  padding:0px !important;
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#loading {
 +
  width: 100%;
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z-index:10000;
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 +
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 +
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 +
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  margin: -120px 0 0 -120px;
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  width: 240px;
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  height: 240px;
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.loader-ring-light {
 +
  width: 240px;
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  -moz-border-radius: 240px;
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  -webkit-border-radius: 240px;
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  border-radius: 240px;
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  -moz-box-shadow: 0 4px 0 #00abf2 inset;
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  -webkit-box-shadow: 0 4px 0 #00abf2 inset;
 +
  box-shadow: 0 4px 0 #00abf2 inset;
 +
  animation: rotate-360 2s linear infinite;
 +
}
  
 +
.loader-ring-track {
 +
  position: absolute;
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  height: 240px;
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  -webkit-border-radius: 240px;
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  border-radius: 240px;
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  -moz-box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
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  box-shadow: 0 0 10px 4px rgba(0, 0, 0, 0.3) inset;
 +
}
  
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<div class="column full_size" >
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@keyframes rotate-360 {
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<h1> Welcome to iGEM 2018! </h1>
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  from {
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    -moz-transform: rotate(0);
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    -ms-transform: rotate(0);
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+
    -webkit-transform: rotate(0);
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<img src="http://placehold.it/1080x320/c4baba/e4dede">
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    -o-transform: rotate(0);
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+
    transform: rotate(0);
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+
  }
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</div>
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  to {
−
 
+
    -moz-transform: rotate(360deg);
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+
    -ms-transform: rotate(360deg);
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<div class="column full_size" >
+
    -webkit-transform: rotate(360deg);
−
 
+
    -o-transform: rotate(360deg);
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<h3>Before you start</h3>
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    transform: rotate(360deg);
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<p> Please read the following pages:</p>
+
  }
−
<ul>
+
}
−
<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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.fullscreenvideo {
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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    position: absolute;
−
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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    top: 70%;
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</ul>
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    left: 50%;
 +
    min-width: 100%;
 +
    min-height: 100%;
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    width: auto;
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    height: auto;
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    z-index: -100;
 +
    -webkit-transform: translateX(-50%) translateY(-50%);
 +
    transform: translateX(-50%) translateY(-50%);
 +
    -webkit-transition: 1s opacity;
 +
    transition: 1s opacity;
 +
}
 +
.videocontainer{
 +
    position: fixed;
 +
    width: 100%;
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    height: 130%;
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    overflow: hidden;
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    z-index: -100;
 +
    top: -25%;
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text-align: center;
 +
}
 +
.videocontainer:before{
 +
    content: "";
 +
    position: absolute;
 +
    width: 100%;
 +
    height: 100%;
 +
    display: block;
 +
    z-index: -10;
 +
    top: 0;
 +
    left: 0;   
 +
}
 +
#PopQC_largr {
 +
display: none;
 +
}
 +
#jun {
 +
display: none;
 +
}
 +
#sihuansu {
 +
display: none;
 +
z-index: 10;
 +
}
 +
#pop_circle {
 +
  display: none;
 +
}
 +
.linkto{
 +
  position: absolute;
 +
  top:72vh;
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  left: 50%;
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  transform: translateX(-50%);
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  -moz-transform: translateX(-50%);
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  -ms-transform: translateX(-50%);
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  -webkit-transform: translateX(-50%);
 +
  z-index: 10;
 +
}
 +
.link2to{
 +
  transform: translateX(-50%);
 +
  -moz-transform: translateX(-50%);
 +
  -ms-transform: translateX(-50%);
 +
  -webkit-transform: translateX(-50%);
 +
  z-index: 10;
 +
}
 +
.a_link {cursor: pointer;}
 +
.btn_video {
 +
  color: white;
 +
  background: none;
 +
  text-align: center;
 +
}
 +
#zhuyetu img:hover {
 +
-webkit-transition: all 0.5s;
 +
-o-transition: all 0.5s;
 +
transition: all 0.5s;
 +
-webkit-transform: scale(1.2);
 +
    -ms-transform: scale(1.2);
 +
        transform: scale(1.2);
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}
 +
</style>
 +
<script type="text/javascript">
 +
$(function(){
 +
var bgVideo=document.getElementById("videohome");
 +
bgVideo.addEventListener('canplaythrough',function(){
 +
              $('#loading').css("display","none");
 +
              $('.loader-ring').css("display","none");
 +
              $('#loading_p').css("display","none");
 +
              $('#PopQC_largr').css("display","block");
 +
              $('#jun').fadeIn(4000);
 +
              $('#sihuansu').fadeIn(4000);
 +
              $('#pop_circle').css("display","block");
 +
    });
 +
});
 +
function scrollupone() {
 +
    $("html, body").animate({
 +
      scrollTop: $("#dinwei").offset().top }, {duration: 700,easing: "swing"});
 +
}
 +
function scrolluptwo() {
 +
    $("html, body").animate({
 +
      scrollTop: $("#dinwei2").offset().top }, {duration: 700,easing: "swing"});
 +
}
 +
</script>
 +
</head>
 +
<body>
 +
<div id="loading"></div>
 +
<div id="loading_p">
 +
<p style="color: white;font-family:myTitle3;font-size:30px;position:relative;top:47%;left:46%">Loading...</p>
 
</div>
 
</div>
  
−
 
+
  <div class="loader-ring">
−
<div class="clear extra_space"></div>
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  <div class="loader-ring-light"></div>
−
<div class="line_divider"></div>
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  <div class="loader-ring-track"></div>
−
<div class="clear extra_space"></div>
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−
 
+
−
 
+
−
 
+
−
<div class="column full_size" >
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−
<h3> Styling your wiki </h3>
+
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
+
−
 
+
 
</div>
 
</div>
 +
  <div class="videocontainer">
 +
        <div class="fullscreenvideo" >
 +
        <video id="videohome" autoplay="autoplay" muted="muted" loop="loop">
 +
          <source src="https://static.igem.org/mediawiki/2018/d/db/T--NKU_CHINA--home_bg.mp4" type="video/mp4">
 +
        </video>
 +
        </div>
 +
  </div>
 +
<main>
 +
    <img src="https://static.igem.org/mediawiki/2018/9/98/T--NKU_CHINA--PopQC_larger.gif" id="PopQC_largr" style="position:absolute;left: 28%;top: -14%;z-index: 10;width: 600px;height: auto;">
 +
    <img src="https://static.igem.org/mediawiki/2018/0/0e/T--NKU_CHINA--pop_circle.gif" id="pop_circle" style="position: absolute;left:34%;top: -3.5%;z-index: 10;">
 +
    <img src="https://static.igem.org/mediawiki/2018/9/9d/T--NKU_CHINA--junfinal.png" id="jun" style="position: absolute;top: 7.5%;left: 20%;width: 450px;height: auto;z-index:20;">
 +
    <img src="https://static.igem.org/mediawiki/2018/f/f9/T--NKU_CHINA--sihuansu.png" id="sihuansu" style="position: absolute;top: 4.5%;left: 55%;width: 500px;height: auto;">
 +
            <div class="container-fluid">
 +
        <div class="row">
 +
          <div class="col-xs-12">
 +
    <img src="https://static.igem.org/mediawiki/2018/f/fb/T--NKU_CHINA--empty.png" class="img-responsive center-block">
 +
        <img  class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/b/bf/T--NKU_CHINA--arrowdown.gif" style="width: 100px;height: auto;cursor:pointer;z-index:1000;position:absolute;top:20%;left:48%;" onclick="scrollupone()">
  
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+
      <div class="container">
−
 
+
        <div class="row">
−
 
+
          <div class="col-xs-12">
−
<div class="clear extra_space"></div>
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            <p><br><br></p>
−
 
+
          </div>
−
 
+
        </div>
−
 
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      </div>
−
<div class="column third_size" >
+
      <div class="container">
−
 
+
        <div class="row">
−
<h3> Uploading pictures and files </h3>
+
          <div class="col-xs-12">
−
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
+
            <h2 style="color: white; font-size: 60px; font-family: myTitle3;text-align: center;margin-top:30px;margin-bottom:20px;padding-top:0;padding-bottom:0;" id="dinwei">Short Video Display</h2>
−
 
+
          </div>
−
 
+
          <div class="col-xs-12 col-xs-push-1">
−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
              <video preload="preload" controls="controls" width="800" height="auto" id="nku_video" poster="https://static.igem.org/mediawiki/2018/b/b2/T--NKU_CHINA--video_image.jpg">
−
 
+
              <source src="https://static.igem.org/mediawiki/2018/b/b6/T--NKU_CHINA--igem_NKU.mp4" type="video/mp4">
−
<div class="button_link">
+
            </video>
−
<a href="https://2018.igem.org/Special:Upload">
+
          </div>
−
UPLOAD FILES
+
          <div class="col-xs-12" style="margin-top: 10px;">
−
</a>
+
              <p style="text-align: center;"><a href="https://youtu.be/Gmr6zBYlpK0" style="color: white;font-size: 25px;">Cilck to watch our video on <span style="color: yellow;">YouTube</span></a></p>
 +
          </div>
 +
        </div>
 +
      </div>
 +
<div class="container">
 +
<div class="row">
 +
<div class="col-xs-12">
 +
        <img  class="img-responsive center-block" src="https://static.igem.org/mediawiki/2018/b/bf/T--NKU_CHINA--arrowdown.gif" style="width: 100px;height: auto;cursor:pointer;" onclick="scrolluptwo()">  
 
</div>
 
</div>
−
 
 
</div>
 
</div>
−
 
−
<div class="column third_size" >
 
−
<h3> Wiki template information </h3>
 
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
−
 
−
</div>
 
−
 
−
 
−
 
−
<div class="column third_size" >
 
−
<div class="highlight decoration_B_full">
 
−
<h3> Editing your wiki </h3>
 
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
−
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
−
 
−
<div class="button_link">
 
−
<a href="https://2018.igem.org/wiki/index.php?title=Team:NKU_CHINA&action=edit">
 
−
EDIT PAGE
 
−
</a>
 
 
</div>
 
</div>
 +
      <div class="container">
 +
        <div class="row">
 +
          <div class="col-xs-12">
 +
            <p id="dinwei2"><br><br><br><br><br><br></p>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="container">
 +
        <div class="row">
 +
          <div class="col-xs-7" id="zhuyetu">
 +
            <img src="https://static.igem.org/mediawiki/2018/e/e3/T--NKU_CHINA--zhuyetu.png" class="img-responsive">
 +
          </div>
 +
          <div class="col-xs-5" style="margin-top: 25px;">
 +
            <p style="color: white;font-size: 23px;line-height: 30px;" style="float: left;text-align: justify;">Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.</p>
 +
          </div>
 +
      </div>
 +
      </div>
 +
      <div class="container">
 +
      <div class="row">
 +
          <div class="col-xs-12" style="margin-top: 20px;">
 +
            <p style="color: white;font-size: 23px;line-height: 30px;text-align: justify;">Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in <i>Bacillus amyloliquefaciens</i> LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— &#947;-PGA.</p>
 +
          </div>
 +
        </div>
 +
      </div>
  
 +
</main>
 +
     
 +
<footer>
 +
<div class="container-fluid myFooter">
 +
<div class="row">
 +
<div class="col-xs-8 col-xs-push-1">
 +
<img src="https://static.igem.org/mediawiki/2018/0/0b/T--NKU_CHINA--duihui_final.png" style="height: 250px; width: auto;">
 +
</div>
 +
<div class="col-xs-4 footer-w3ls">
 +
<h3 style="color: white; font-size: 30px;">Contacts</h3>
 +
<ul class="social-icons1" style="margin:0 auto;padding-top:0;">
 +
<li><a href="https://www.facebook.com/iGEMNKU2018/"><i class="fa fa-facebook" aria-hidden="true"></i></a></li>
 +
<li><a href="https://twitter.com/iGEMNKU2018"><i class="fa fa-twitter" aria-hidden="true"></i></a></li>
 +
<li><a href="mailto:nkuigem2018@163.com"><i class="fa fa fa-envelope-o" aria-hidden="true"></i></a></li>
 +
    </ul>
 +
    <h3 style="color: white; font-size: 30px;">Address</h3>
 +
                <address style="color: white; font-size: 17px;">
 +
                    Nankai University</br>
 +
                    No.94 Weijin Road, Nankai District</br>
 +
                    Tianjin, P.R.China 300071</br>
 +
                </address>
 +
            </div>
 +
            <div class="col-xs-12">
 +
            <p class="Copyright" style="text-align: center; color: white;">Copyright © Team NKU_CHINA 2018</p>
 +
        </div>
 +
    </div>
 +
</footer>
  
−
</div>
+
<a href="#0" class="cd-top">Top</a>
−
</div>
+
</body>
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
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<div class="clear extra_space"></div>
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<div class="line_divider"></div>
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<div class="clear extra_space"></div>
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+
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+
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+
−
<div class="column two_thirds_size" >
+
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<h3>Tips</h3>
+
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
−
<ul>
+
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>
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</div>
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−
 
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration</h3>
+
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
−
<ul>
+
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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</html>

Latest revision as of 00:32, 18 October 2018

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Short Video Display

Cilck to watch our video on YouTube







Population Quality Control (PopQC) system, is a new approach designed for biosynthesis yield enhancement, based on non-genetic cell-to-cell variation, which includes unequal cell division, different gene copy numbers, epigenetic modifications, random gene expression, volatile RNA stability, protein activity, etc.

Because of those differences, different cells in a single colony will have considerable variations in protein and metabolite concentrations. Therefore, in cell cultures there will be both high- and low-producers, and the intrinsic low-producers might cause suboptimal ensemble biosynthesis. The elimination of low-producers can realize the efficient utilization of substrates and high yield of target products. Thus, it has been proved to be an efficient way for biosynthesis being more suitable for large-scale industrial production. In our project, PopQC was designed as a plasmid-based gene circuit in Bacillus amyloliquefaciens LL3, which continuously selects high-performing cells in order to further improve the yield of target metabolite— glutamate, and then the secondary metabolites— γ-PGA.

Contacts

Address

Nankai University
No.94 Weijin Road, Nankai District
Tianjin, P.R.China 300071

Copyright © Team NKU_CHINA 2018

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