Difference between revisions of "Team:Newcastle/Parts"

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                    <span>Scroll Down</span>
 
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                            <h3 class="subhead">EXPERIMENTS</h3>
+
                   
 
                 <h1 class="display-2">Parts Used</h1>
 
                 <h1 class="display-2">Parts Used</h1>
 
             </div>
 
             </div>
 +
<p><font class="3"><center>Here we list the parts used in our study.</center></font></p>
 +
<br><br>
 +
<h2>New Parts</h2>
 
         </div> <!-- end section-header -->
 
         </div> <!-- end section-header -->
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<table style=''width:0%>
 +
<tr>
 +
      <th>Name</th>
 +
      <th>Type</th>
 +
      <th>Description</th>
 +
      <th>Designer</th>
 +
      <th>Length(bp)</th>
 +
  </tr>
 +
<tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797002" class='black'>BBa_K2797002</a></td>
 +
      <td>Composite</td>
 +
      <td>Composite part for streptomycin resistance</td>
 +
      <td>Frank Eardley</td>
 +
      <td>908</td>
 +
  </tr>
 +
<tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797014" class='black'>BBa_K2797014</a></td>
 +
      <td>Coding sequence</td>
 +
      <td>Catalyses the formation of p-coumaric acid from tyrosine</td>
 +
      <td>Heather Bottomley</td>
 +
      <td>1596</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797003" class='black'>BBa_K2797003</a></td>
 +
      <td>Basic</td>
 +
      <td>mNeonGreen fluorescent protein gene codon optimised for expression in <i>E. coli</i></td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>711</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797004" class='black'>BBa_K2797004</a></td>
 +
      <td>Composite</td>
 +
      <td>  Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>909</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797005"class='black' >BBa_K2797005</a></td>
 +
      <td>Composite</td>
 +
      <td>  Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>909</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797006" class='black'>BBa_K2797006</a></td>
 +
      <td>Composite</td>
 +
      <td>  Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>909</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797007" class='black'>BBa_K2797007</a></td>
 +
      <td>Composite</td>
 +
      <td>  Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>909</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797008" class='black'>BBa_K2797008</a></td>
 +
      <td>Composite</td>
 +
      <td>  Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>910</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2797013" class='black'>BBa_K2797013</a></td>
 +
      <td>Plasmid vector</td>
 +
      <td> High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard</td>
 +
      <td>Kyle Stanforth</td>
 +
      <td>3073</td>
 +
  </tr>
 +
</table>
 +
 +
        <div class="row section-header has-bottom-sep" data-aos="fade-up">
 +
                <div class="col-full">
 +
<h2>Existing Parts</h2></div></div>
 
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   <tr>
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       <td><a href="http://parts.igem.org/Part:BBa_I20270">BBa_I20270</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_I20270" class='black'>BBa_I20270</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>Positive control for the interLab study</td>
 
       <td>Positive control for the interLab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J364000">BBa_J364000</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J364000" class='black'>BBa_J364000</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>GFP gene for the interlab study</td>
 
       <td>GFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J364001">BBa_J364001</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J364001" class='black'>BBa_J364001</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>GFP gene for the interlab study</td>
 
       <td>GFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J364002">BBa_J364002</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J364002" class='black'>BBa_J364002</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>GFP gene for the interlab study</td>
 
       <td>GFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J364007">BBa_J364007</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J364007" class='black'>BBa_J364007</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>GFP gene for the interlab study</td>
 
       <td>GFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J364008">BBa_J364008</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J364008" class='black'>BBa_J364008</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>GFP gene for the interlab study</td>
 
       <td>GFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J364009">BBa_J364009</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J364009" class='black'>BBa_J364009</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>GFP gene for the interlab study</td>
 
       <td>GFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_R0040">BBa_R0040</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_R0040" class='black'>BBa_R0040</a></td>
 
       <td>Regulatory sequence</td>
 
       <td>Regulatory sequence</td>
 
       <td>TetR repressible promoter for the interlab study</td>
 
       <td>TetR repressible promoter for the interlab study</td>
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   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_J04450" class='black'>BBa_J04450</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>RFP gene for the interlab study</td>
 
       <td>RFP gene for the interlab study</td>
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   </tr>
 
   </tr>
 
  <tr>
 
  <tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K1033001">BBa_K1033001</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K1033001" class='black'>BBa_K1033001</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>Catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A</td>
 
       <td>Catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A</td>
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       <td>1708</td>
 
       <td>1708</td>
 
   </tr>
 
   </tr>
 +
 
<tr>
 
<tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K2797014">BBa_K2797014</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K1497000" class='black'>BBa_K1497000</a></td>
−
      <td>Coding sequence</td>
+
−
      <td>Catalyses the formation of p-coumaric acid from tyrosine</td>
+
−
      <td>Heather Bottomley</td>
+
−
      <td>1596</td>
+
−
  </tr>
+
−
<tr>
+
−
      <td><a href="http://parts.igem.org/Part:BBa_K1497000">BBa_K1497000</a></td>
+
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>Catalyses the reaction from naringenin chalcone to naringenin.</td>
 
       <td>Catalyses the reaction from naringenin chalcone to naringenin.</td>
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   </tr>
 
   </tr>
 
<tr>
 
<tr>
−
       <td><a href="http://parts.igem.org/Part:BBa_K1497001">BBa_K1497001</a></td>
+
       <td><a href="http://parts.igem.org/Part:BBa_K1497001" class='black'>BBa_K1497001</a></td>
 
       <td>Coding sequence</td>
 
       <td>Coding sequence</td>
 
       <td>Catalyses the reaction from 4-Coumaroyl-CoA to naringenin chalcone.</td>
 
       <td>Catalyses the reaction from 4-Coumaroyl-CoA to naringenin chalcone.</td>
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       <td>1197</td>
 
       <td>1197</td>
 
   </tr>
 
   </tr>
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797002">BBa_K2797002</a></td>
 
−
      <td>Composite</td>
 
−
      <td>Composite part for streptomycin resistance</td>
 
−
      <td>Frank Eardley</td>
 
−
      <td>908</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797003">BBa_K2797003</a></td>
 
−
      <td>Basic</td>
 
−
      <td>mNeonGreen fluorescent protein gene codon optimised for expression in <i>E. coli</i></td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>711</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797004">BBa_K2797004</a></td>
 
−
      <td>Composite</td>
 
−
      <td>  Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>909</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797005">BBa_K2797005</a></td>
 
−
      <td>Composite</td>
 
−
      <td>  Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>909</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797006">BBa_K2797006</a></td>
 
−
      <td>Composite</td>
 
−
      <td>  Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>909</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797007">BBa_K2797007</a></td>
 
−
      <td>Composite</td>
 
−
      <td>  Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>909</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797008">BBa_K2797008</a></td>
 
−
      <td>Composite</td>
 
−
      <td>  Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter </td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>910</td>
 
−
  </tr>
 
−
  <tr>
 
−
      <td><a href="http://parts.igem.org/Part:BBa_K2797013">BBa_K2797013</a></td>
 
−
      <td>Plasmid vector</td>
 
−
      <td> High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard</td>
 
−
      <td>Kyle Stanforth</td>
 
−
      <td>3073</td>
 
−
  </tr>
 
 
</table>
 
</table>
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      <groupparts>iGEM18 Newcastle</groupparts>
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<section id='team' class="s-services">
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                            <br>
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<br>
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<br>
 +
<br>
 +
<h3 class="subhead"></h3>
 +
                <h1 class="display-2">References & Attributions</h1>
 +
            </div>
 +
 +
        </div>
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<button class="collapsible">Click for References and Attributions</button>
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                <div class="col-full">
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<p class="about-para"><font size="2"><strong>Attributions: Frank Eardley, Heather Bottomley and Kyle Stanforth
 +
</strong><font></p>
 +
 +
 +
 +
 +
  
 +
</div>
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</section>
 
    
 
    
 
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Latest revision as of 00:33, 18 October 2018

Alternative Roots/Protocols

Alternative Roots

Parts Overview

Overview

Parts Used

Here we list the parts used in our study.



New Parts

Name Type Description Designer Length(bp)
BBa_K2797002 Composite Composite part for streptomycin resistance Frank Eardley 908
BBa_K2797014 Coding sequence Catalyses the formation of p-coumaric acid from tyrosine Heather Bottomley 1596
BBa_K2797003 Basic mNeonGreen fluorescent protein gene codon optimised for expression in E. coli Kyle Stanforth 711
BBa_K2797004 Composite Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797005 Composite Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797006 Composite Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797007 Composite Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797008 Composite Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 910
BBa_K2797013 Plasmid vector High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard Kyle Stanforth 3073

Existing Parts

Name Type Description Designer Length(bp)
BBa_I20270 Coding sequence Positive control for the interLab study Jason Kelly 919
BBa_J364000 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364001 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364002 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364007 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364008 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364009 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_R0040 Regulatory sequence TetR repressible promoter for the interlab study June Rhee, Connie Tao, Ty Thomson, Louis Waldman 54
BBa_J04450 Coding sequence RFP gene for the interlab study Tamar Odle 1069
BBa_K1033001 Coding sequence Catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A Karl Holdar 1708
BBa_K1497000 Coding sequence Catalyses the reaction from naringenin chalcone to naringenin. Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen 726
BBa_K1497001 Coding sequence Catalyses the reaction from 4-Coumaroyl-CoA to naringenin chalcone. Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen 1197




References & Attributions

Attributions: Frank Eardley, Heather Bottomley and Kyle Stanforth