Difference between revisions of "Team:Peking/Safety"

 
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     <head>
 
     <head>
 
         <!--- Basic Page Needs========================================================================= -->
 
         <!--- Basic Page Needs========================================================================= -->
−
         <meta charset="utf-8">
+
         <meta charset="utf-8"/>
−
            <title>Overview</title>
+
        <title></title>
−
            <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.2, user-scalable=yes" />
+
        <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.2, user-scalable=yes" />
−
            <meta name="description" content="Wiki of Peking iGEM 2016" />
+
        <meta name="description" content="Wiki of Peking iGEM 2018" />
−
            <meta name="author" content="Li Jiamian & Wang Yuqing">
+
        <meta name="author" content="Peking iGEM"/>
−
                <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
        <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
−
                <!-- Mobile Specific Metas===================================================================== -->
+
        <!-- Mobile Specific Metas===================================================================== -->
−
                <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
+
        <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1"/>
−
                    <!-- Fix  Overwrite the original iGEM style=================================================== -->
+
        <!-- Fix  Overwrite the original iGEM style=================================================== -->
−
                    <link href="https://2016.igem.org/Template:Peking/css/fix?action=raw&ctype=text/css" rel="stylesheet" />
+
        <link href="https://2018.igem.org/Template:Peking/css/fix?action=raw&ctype=text/css" rel="stylesheet" />
−
                    <!-- CSS======================================================================================= -->
+
        <!-- CSS======================================================================================= -->
−
                    <link href="https://2016.igem.org/Template:Peking/css/bootstrap_min?action=raw&ctype=text/css" rel="stylesheet" />
+
        <link href="https://2018.igem.org/Template:Peking/css/bootstrap_min?action=raw&ctype=text/css" rel="stylesheet" />
−
                    <link href="https://2016.igem.org/Template:Peking/css/style?action=raw&ctype=text/css" rel="stylesheet" />
+
        <link href="https://2018.igem.org/Template:Peking/css/style?action=raw&ctype=text/css" rel="stylesheet" />
−
                    <!-- CSS======================================================================================= -->
+
        <!-- CSS======================================================================================= -->
−
                    <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/default?action=raw&ctype=text/css">
+
        <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/default?action=raw&ctype=text/css"/>
−
                        <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/layout?action=raw&ctype=text/css">
+
        <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/layout?action=raw&ctype=text/css"/>
−
                            <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/media-queries?action=raw&ctype=text/css">
+
        <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/media-queries?action=raw&ctype=text/css"/>
−
                                <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/priorstyle?action=raw&ctype=text/css">
+
        <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/notebook_panel?action=raw&ctype=text/css"/>
−
                                    <style>
+
        <style>
−
                                        .home_img{
+
                        body { background: #D2D8D8 url(https://static.igem.org/mediawiki/2018/7/78/T--Peking--images_bodyBackground.jpeg); background-attachment:fixed;}
−
                                            padding: 10px;
+
            .texttitle{
−
                                            margin: 10px;
+
                color:#6495ED;
−
                                            width: 420px;
+
                font-size: 38px;
−
                                            float: left;
+
                line-height: 48px;
−
                                            height: 300px;
+
                margin-bottom: 12px;
−
                                        }
+
                font-family: raleway-bold, sans-serif !important;
−
                                    .home_text{
+
                background: transparent;
−
                                        padding-top: 20px;
+
                letter-spacing: 3px;
−
                                        margin: 10px;
+
                text-transform: uppercase;
−
                                        float: left;
+
                font-weight: 350;
−
                                        height: 300px;
+
                text-align: center;
−
                                    }
+
                padding-top:40px;
−
                                    .texttitle{
+
            }
−
                                        color: #11abb0;
+
        sup{font-size:11px;}
−
                                        font-size: 38px;
+
        .references{margin-top:150px;margin-bottom:40px;}
−
                                        line-height: 48px;
+
        .references p{font-size:14px !important; color:#666161 !important;}
−
                                        margin-bottom: 12px;
+
        .classic-title {font-weight: 300;padding-top:30px;}
−
                                        font-family: raleway-bold, sans-serif;
+
        .classic-title span {
−
                                        background: transparent;
+
            padding-bottom: 8px;
−
                                        letter-spacing: 3px;
+
            border-bottom: 1px solid #383232;
−
                                        text-transform: uppercase;
+
            font-weight: 400;
−
                                        font-weight: 350;
+
        }
−
                                        text-align: center;
+
        figure{margin-top:40px;margin-bottom:40px;height:auto;}
−
                                    }
+
        .anchor{padding-top:100px;margin-top:-100px;}
−
                                    sup{
+
            </style>
−
                                        font-size:11px;
+
       
−
                                    }
+
    </head>
−
                                    .references{margin-top:150px;margin-bottom:40px;}
+
−
                                    .references p{font-size:14px !important; color:#666161 !important;}
+
−
                                   
+
−
                                    .classic-title {font-weight: 300;}
+
−
                                    .classic-title span {
+
−
                                        padding-bottom: 8px;
+
−
                                        border-bottom: 1px solid #383232;
+
−
                                        font-weight: 400;
+
−
                                    }
+
−
                                    figure{margin-top:40px;margin-bottom:40px;height:auto;}
+
−
                                        </style>
+
−
                                   
+
−
                                    </head>
+
−
   
+
 
     <body>
 
     <body>
 +
        <!--sidebar 引用==============================================================================-->
 +
        <style>
 +
            #primary span{
 +
                display:block;
 +
                word-break:break-all
 +
            }
 +
       
 +
        #page-wrap {
 +
            width: 25%;
 +
            margin: 0px;
 +
            position: relative;
 +
        }
 +
       
 +
        #sidebar {
 +
            width: 25%;
 +
            margin-left: 0px;
 +
        }
 +
        @media (min-width:1024px){
 +
            #sidebar{position:relative;top:120px;max-width:200px;}}
 +
        @media (max-width: 1023px){
 +
            #sidebar{display:none;
 +
            }
 +
            #page-wrap{display:none;}
 +
        }
 +
        </style>
 +
       
 +
       
 +
        <script type="text/javascript">
 +
            function menuFixed(id){
 +
                var obj = document.getElementById(id);
 +
                var _getHeight = obj.offsetTop;
 +
               
 +
                window.onscroll = function(){
 +
                    changePos(id,_getHeight);
 +
                }
 +
            }
 +
        function changePos(id,height){
 +
            var obj = document.getElementById(id);var windowBottom = $(window).scrollTop() + $(window).innerHeight();
 +
            if(w>=1024){
 +
                if($(window).scrollTop() + $(window).height() > $(document).height() - 230){
 +
                    $('#sidebar').fadeOut("fast");}else{$('#sidebar').fadeIn("fast");}
 +
            }
 +
            var scrollTop = document.documentElement.scrollTop || document.body.scrollTop -230;
 +
            var windowBottom = $(window).scrollTop() + $(window).innerHeight();
 +
            var w = window.innerWidth;
 +
           
 +
            if(scrollTop < height){ obj.style.position = 'relative';
 +
            }else{
 +
                obj.style.position = 'fixed';
 +
            }
 +
        }
 +
        </script>
 +
       
 +
        <script type="text/javascript">
 +
            window.onload = function(){
 +
                menuFixed('sidebar');
 +
            }
 +
        </script>
 +
        <script>
 +
            function naver(id){
 +
                var obj=document.getElementById(id);
 +
                var oPos=obj.offsetTop;
 +
                return window.scrollTo(0,oPos+250);
 +
            }
 +
        </script>
 +
        <!--sidebar 引用 end ==============================================================================-->
 +
        <!--panel 引用==================================================================================-->
 +
        <style type="text/css">
 +
            .panel-default .panel-heading a{
 +
                text-decoration: none;
 +
                display:block;
 +
                padding:10px;
 +
            }
 +
        .panel-heading.panel-title{
 +
            text-decoration: none;
 +
            padding-top:0px;
 +
            padding-bottom:0px;
 +
            padding-left:0px;
 +
            padding-right:0px;
 +
            text-align:center;
 +
            font-size:19px;
 +
           
 +
        }
 +
        a[aria-expanded="true"] {
 +
            background-color:rgba(70, 73, 76, 0.95);
 +
            text-decoration: none;
 +
            color:white;
 +
        }
 +
       
 +
        .panel-default .panel-heading a[aria-expanded="false"]{
 +
            -o-transition: background-color 1s linear;
 +
            -moz-transition: background-color 1s linear;
 +
            -khtml-transition: background-color 1s linear;
 +
            -webkit-transition: background-color 1s linear;
 +
            -ms-transition: background-color 1s linear;
 +
            transition: background-color 1s linear;
 +
        }
 +
        .panel-default .panel-heading a[aria-expanded="false"]:hover{
 +
            background-color:rgba(70, 73, 76, 0.95);
 +
            text-decoration: none;
 +
            color:white;
 +
        }
 +
        .panel-default .panel-heading a[aria-expanded="true"]{
 +
            -o-transition: opacity 1s linear;
 +
            -moz-transition: opacity 1s linear;
 +
            -khtml-transition: opacity 1s linear;
 +
            -webkit-transition: opacity 1s linear;
 +
            -ms-transition: opacity 1s linear;
 +
            transition: opacity 0.7s linear;
 +
        }
 +
        .panel-default .panel-heading a[aria-expanded="true"]:hover{
 +
            opacity:0.7;
 +
        }
 +
       
 +
        .coll p a{
 +
            color:#5c9085 !important;
 +
        }
 +
        .coll p a:hover{
 +
            color:#11abb0 !important;
 +
        }
 +
        .coll {
 +
            width:100%;
 +
            float:left;
 +
        }
 +
        .coll .info {
 +
            width:6%;
 +
            font-size:11px;
 +
            color:#a4a4a4;
 +
            margin-top:0px;
 +
            float:left;
 +
           
 +
        }
 +
        .coll .content {
 +
            width:94%;
 +
            float:left;
 +
            margin-left:40px;
 +
            margin-top:-3px;
 +
        }
 +
        .coll .ordi {
 +
            width;100%;
 +
            margin:0px 0px 0px 0px;
 +
            font-size:35px;
 +
            font-style:italic;
 +
            float:left;
 +
            color:#11abb0;
 +
            opacity:0.8;
 +
        }
 +
       
 +
            </style>
 +
       
 +
        <script type="text/javascript">
 +
            $(document).ready(function(){
 +
                              $("#button1").click(function(){
 +
                                                  $(".panel-collapse").collapse("show");
 +
                                                  });
 +
                              });
 +
                              $(document).ready(function(){
 +
                                                $("#button2").click(function(){
 +
                                                                    $(".panel-collapse").collapse("hide");
 +
                                                                    });
 +
                                                });
 +
                                                $("#notebook").addClass("navbar-active");
 +
            </script>
 +
        <!--panel 引用 end ==================-->
 +
       
 
           <!-- Navigation -->
 
           <!-- Navigation -->
 
         <div id="navigation" class="navbar navbar-fixed-top">
 
         <div id="navigation" class="navbar navbar-fixed-top">
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                     </a>
 
                     </a>
 
                     <div id="logo" style="max-width:0px"><a class="" href="https://2018.igem.org/Team:Peking"><img
 
                     <div id="logo" style="max-width:0px"><a class="" href="https://2018.igem.org/Team:Peking"><img
−
                     src="https://static.igem.org/mediawiki/2018/e/e2/T--Peking--_toolbar.jpeg" width="45%"></a></div>
+
                     src="https://static.igem.org/mediawiki/2018/3/36/T--Peking--Logo.png"></a></div>
 
                    
 
                    
 
                     <div class="nav-collapse collapse">
 
                     <div class="nav-collapse collapse">
−
                         <ul class="nav">
+
                         <ul class="nav">                          
−
                             <li class="dropdown menu-1"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;Project</a>
+
 
 +
                             <li class="menu-1"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking">Home</a>
 +
                            </li>
 +
 
 +
                            <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Project</a>
 
                                 <ul class="dropdown-menu">
 
                                 <ul class="dropdown-menu">
−
                                     <li><a href="https://2018.igem.org/Team:Peking/Project">Project</a></li>
+
                                     <li><a href="https://2018.igem.org/Team:Peking/Project" class="barfont1">Description</a></li>
−
                                    <li><a href="https://2018.igem.org/Team:Peking/Description" class="barfont1">Description</a></li>
+
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li>
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li>
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li>
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Peking/Future_Plan" class="barfont1">Future Plan</a></li>
+
                                     <li><a href="https://2018.igem.org/Team:Peking/Perspective" class="barfont1">Perspective</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
−
                             <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Data Page</a>
+
                             <li class="menu-3"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Model">Modeling</a>
−
                                <ul class="dropdown-menu">
+
−
                                    <li><a href="https://2018.igem.org/Team:Peking/Results">Data Page</a></li>
+
−
                                    <li><a href="https://2018.igem.org/Team:Peking/Phase_Separation_D" class="barfont1">Phase Separation</a></li>
+
−
                                    <li><a href="https://2018.igem.org/Team:Peking/Functions_D" class="barfont1">Functions</a></li>
+
−
                                </ul>
+
 
                             </li>
 
                             </li>
−
                             <li class="dropdown menu-3"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Model</a>
+
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                             <li class="menu-6"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Human_Practices">Human Practices</a>
 
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                                 <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Collaborations</a>
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                                     </ul>
 
                                     </ul>
 
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                    <h1>Safety</h1>
 +
                    <p class="title1" style="text-align:justify; text-justify:inter-ideograph;">Peking iGEM 2018’s project is a breakthrough attempt to construct a new platform with phase separation system abbreviated to SPOT. With the SPOT system, we hope to achieve various biological functions such as a reaction hub or a sensor for plant hormone molecules. Here we will share our experiences with safety control.</p>
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                                    <h4><a href="https://2018.igem.org/Team:Peking/Team_Members">&bull;Team&nbsp;Members</a></h4>
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                                    <h4><a href="https://2018.igem.org/Team:Peking/Attributions">&bull;Attributions</a></h4>
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                                    <h4><a href="https://2018.igem.org/Team:Peking/Notebook">&bull;Notebook</a></h4>
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                                    <h4><a href="https://2018.igem.org/Team:Peking/Safety">&bull;Safety</a></h4>
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                                    <h4><a href="https://2018.igem.org/Team:Peking/Acknowledgement">&bull;Acknowledgement</a></h4>
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                            <div class="texttitle">Safe project design
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                                    <p>Our SPOT contains two main applications. One is to use the SPOT as a reaction hub to regulate various biochemical reactions. We have demonstrated that if we integrated three enzymes which could synthesize β-carotene into SPOT system, the production of  β-carotene in yeasts will be enhanced if phase separation happens. In the future, if we use the yeasts to manufacture β-carotene in a large scale, we can sequester the yeasts in fermenters to prevented recombinant yeasts or recombinant DNA from flowing out into the environment. The other is to use our SPOT as a sensor. We achieved that if there are ABA or Rapamycin molecules in the environment, phase separation would happen. If we want to use the sensor in reality, we can package the yeast cells into resins. It will help us to observe the SPOT formation by microscopy, and limit yeast escape. In both two reality scenes, escape of microbes is prohibited. </p>
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                            <div class="texttitle">Safe materials
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                                    <p>In our experiments, we chose E. coli as the chassis for molecular cloning, and S. cerevisiae as SPOT formation chassis. These two species are well known and non-pathogenic. Our bioparts are constructed by collecting segments from safe plasmid by which means we avoid using unknown segments. Those parts which have potential to cause environment problem, animal and plant disease, ecosystem changing are strictly prohibited.</p>
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                            <div class="texttitle">Safe lab work
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                                    <p>As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:</p>
 +
<p>1.Duplicating a key to the laboratory without permission was strictly prohibited.</p>
 +
<p>2.All involved participants needed to understand the experiment completely before commencing work.</p>
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<p>3.All experimenters had to wear rubber gloves.</p>
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<p>4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.</p>
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<p>5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.</p>
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<p>6.All liquid and solid waste potentially containing living organism was sterilized.</p>
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<p>7.The entire lab was sterilized using UV-light every week.</p>
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<p>8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.
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</p>
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                            <div class="texttitle">Safe shipment
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                                    <p>In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOTags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.
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</p>
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Latest revision as of 00:41, 18 October 2018

Safety

Peking iGEM 2018’s project is a breakthrough attempt to construct a new platform with phase separation system abbreviated to SPOT. With the SPOT system, we hope to achieve various biological functions such as a reaction hub or a sensor for plant hormone molecules. Here we will share our experiences with safety control.

Safe project design

Our SPOT contains two main applications. One is to use the SPOT as a reaction hub to regulate various biochemical reactions. We have demonstrated that if we integrated three enzymes which could synthesize β-carotene into SPOT system, the production of β-carotene in yeasts will be enhanced if phase separation happens. In the future, if we use the yeasts to manufacture β-carotene in a large scale, we can sequester the yeasts in fermenters to prevented recombinant yeasts or recombinant DNA from flowing out into the environment. The other is to use our SPOT as a sensor. We achieved that if there are ABA or Rapamycin molecules in the environment, phase separation would happen. If we want to use the sensor in reality, we can package the yeast cells into resins. It will help us to observe the SPOT formation by microscopy, and limit yeast escape. In both two reality scenes, escape of microbes is prohibited.

Safe materials

In our experiments, we chose E. coli as the chassis for molecular cloning, and S. cerevisiae as SPOT formation chassis. These two species are well known and non-pathogenic. Our bioparts are constructed by collecting segments from safe plasmid by which means we avoid using unknown segments. Those parts which have potential to cause environment problem, animal and plant disease, ecosystem changing are strictly prohibited.

Safe lab work

As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:

1.Duplicating a key to the laboratory without permission was strictly prohibited.

2.All involved participants needed to understand the experiment completely before commencing work.

3.All experimenters had to wear rubber gloves.

4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.

5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.

6.All liquid and solid waste potentially containing living organism was sterilized.

7.The entire lab was sterilized using UV-light every week.

8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.

Safe shipment

In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOTags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.