Difference between revisions of "Team:Peking/Safety"

 
(19 intermediate revisions by 4 users not shown)
Line 9: Line 9:
 
         <title></title>
 
         <title></title>
 
         <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.2, user-scalable=yes" />
 
         <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.2, user-scalable=yes" />
−
         <meta name="description" content="Wiki of Peking iGEM 2016" />
+
         <meta name="description" content="Wiki of Peking iGEM 2018" />
−
         <meta name="author" content="Li Jiamian & Wang Yuqing"/>
+
         <meta name="author" content="Peking iGEM"/>
 
         <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 
         <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 
         <!-- Mobile Specific Metas===================================================================== -->
 
         <!-- Mobile Specific Metas===================================================================== -->
 
         <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1"/>
 
         <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1"/>
 
         <!-- Fix  Overwrite the original iGEM style=================================================== -->
 
         <!-- Fix  Overwrite the original iGEM style=================================================== -->
−
         <link href="https://2016.igem.org/Template:Peking/css/fix?action=raw&ctype=text/css" rel="stylesheet" />
+
         <link href="https://2018.igem.org/Template:Peking/css/fix?action=raw&ctype=text/css" rel="stylesheet" />
 
         <!-- CSS======================================================================================= -->
 
         <!-- CSS======================================================================================= -->
−
         <link href="https://2016.igem.org/Template:Peking/css/bootstrap_min?action=raw&ctype=text/css" rel="stylesheet" />
+
         <link href="https://2018.igem.org/Template:Peking/css/bootstrap_min?action=raw&ctype=text/css" rel="stylesheet" />
−
         <link href="https://2016.igem.org/Template:Peking/css/style?action=raw&ctype=text/css" rel="stylesheet" />
+
         <link href="https://2018.igem.org/Template:Peking/css/style?action=raw&ctype=text/css" rel="stylesheet" />
 
         <!-- CSS======================================================================================= -->
 
         <!-- CSS======================================================================================= -->
−
         <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/default?action=raw&ctype=text/css"/>
+
         <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/default?action=raw&ctype=text/css"/>
−
         <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/layout?action=raw&ctype=text/css"/>
+
         <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/layout?action=raw&ctype=text/css"/>
−
         <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/media-queries?action=raw&ctype=text/css"/>
+
         <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/media-queries?action=raw&ctype=text/css"/>
−
         <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/notebook_panel?action=raw&ctype=text/css"/>
+
         <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/notebook_panel?action=raw&ctype=text/css"/>
 
         <style>
 
         <style>
 +
                        body { background: #D2D8D8 url(https://static.igem.org/mediawiki/2018/7/78/T--Peking--images_bodyBackground.jpeg); background-attachment:fixed;}
 
             .texttitle{
 
             .texttitle{
−
                 color: #11abb0;
+
                 color:#6495ED;
 
                 font-size: 38px;
 
                 font-size: 38px;
 
                 line-height: 48px;
 
                 line-height: 48px;
Line 238: Line 239:
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li>
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li>
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li>
 
                                     <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Peking/Prospective" class="barfont1">Prospective</a></li>
+
                                     <li><a href="https://2018.igem.org/Team:Peking/Perspective" class="barfont1">Perspective</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
−
                             <li class="dropdown menu-3"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Modeling</a>
+
                             <li class="menu-3"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Model">Modeling</a>
−
                                <ul class="dropdown-menu">
+
−
                                    <li><a href="https://2018.igem.org/Team:Peking/Model">Overview</a></li>
+
−
                                    <li><a href="https://2018.igem.org/Team:Peking/SPOT_Formation" class="barfont1">SPOT Formation</a></li>
+
−
                                    <li><a href="https://2018.igem.org/Team:Peking/Application" class="barfont1">Application</a></li>
+
−
                                </ul>
+
 
                             </li>
 
                             </li>
 
                             <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a>
 
                             <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a>
Line 252: Line 248:
  
  
−
                                <li class="dropdown menu-6"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practices</a>
+
                            <li class="menu-6"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Human_Practices">Human Practices</a>
−
                                    <ul class="dropdown-menu">
+
                            </li>
−
                                        <li><a href="https://2018.igem.org/Team:Peking/Human_Practices" class="barfont1">Overview</a></li>
+
−
                                        <li><a href="https://2018.igem.org/Team:Peking/Statistics" class="barfont1">Statistics</a></li>
+
−
                                        <li><a href="https://2018.igem.org/Team:Peking/Public_Engagement" class="barfont1">Public Engagement</a></li>
+
−
                                        <li><a href="https://2018.igem.org/Team:Peking/Other" class="barfont1">Other</a></li>
+
−
                                    </ul>
+
−
                                </li>
+
 
                                 <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achievement</a>
 
                                 <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achievement</a>
 
                                     <ul class="dropdown-menu">
 
                                     <ul class="dropdown-menu">
Line 276: Line 266:
 
                                         <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li>
 
                                         <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li>
 
                                         <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li>
 
                                         <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li>
−
                                                                            <li><a href="https://2018.igem.org/Team:Peking/Acknowledgement" class="barfont1">Acknowledgement</a></li></ul>
+
                                        <li><a href="https://2018.igem.org/Team:Peking/Acknowledgement" class="barfont1">Acknowledgement</a></li>
 +
                                       
 +
                                    </ul>
 
                                 </li>
 
                                 </li>
 
                                 </div>
 
                                 </div>
Line 307: Line 299:
 
                             <div id="page-wrap">
 
                             <div id="page-wrap">
 
                                 <div id="sidebar" style="color:#000000">
 
                                 <div id="sidebar" style="color:#000000">
−
                                     <h4><a href="javascript:void(0);" onclick="naver('A')">Overview</a></h4>
+
                                   
−
                                     <h4><a href="javascript:void(0);" onclick="naver('B')">Phase&nbsp;Separation</a></h4>
+
                                     <h4><a href="https://2018.igem.org/Team:Peking/Team_Members">&bull;Team&nbsp;Members</a></h4>
−
                                     <ul>
+
                                     <h4><a href="https://2018.igem.org/Team:Peking/Attributions">&bull;Attributions</a></h4>
−
                                        <li><a href="javascript:void(0);" onclick="naver('B1')">Spontaneous</a></li>
+
                             
−
                                        <li><a href="javascript:void(0);" onclick="naver('B2')">The&nbsp;formation</a></li>
+
                                     <h4><a href="https://2018.igem.org/Team:Peking/Notebook">&bull;Notebook</a></h4>
−
                                    </ul>
+
                                    <h4><a href="https://2018.igem.org/Team:Peking/Collaborations">&bull;Collaborations</a></h4>
−
                                     <h4><a href="javascript:void(0);" onclick="naver('C')">Functional&nbsp;Organelles</a></h4>
+
                                     <h4><a href="https://2018.igem.org/Team:Peking/Safety">&bull;Safety</a></h4>
−
                                     <h4><a href="javascript:void(0);" onclick="naver('D')">Perspective</a></h4>
+
                                     <h4><a href="https://2018.igem.org/Team:Peking/Acknowledgement">&bull;Acknowledgement</a></h4>
 +
                             
 
                                 </div>
 
                                 </div>
 
                             </div>
 
                             </div>
Line 357: Line 350:
 
                                  
 
                                  
 
                                 <div class="content">
 
                                 <div class="content">
−
                                     <p>As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:
+
                                     <p>As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:</p>
−
1.Duplicating a key to the laboratory without permission was strictly prohibited.
+
<p>1.Duplicating a key to the laboratory without permission was strictly prohibited.</p>
−
2.All involved participants needed to understand the experiment completely before commencing work.
+
<p>2.All involved participants needed to understand the experiment completely before commencing work.</p>
−
3.All experimenters had to wear rubber gloves.
+
<p>3.All experimenters had to wear rubber gloves.</p>
−
4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.
+
<p>4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.</p>
−
5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.
+
<p>5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.</p>
−
6.All liquid and solid waste potentially containing living organism was sterilized.
+
<p>6.All liquid and solid waste potentially containing living organism was sterilized.</p>
−
7.The entire lab was sterilized using UV-light every week.
+
<p>7.The entire lab was sterilized using UV-light every week.</p>
−
8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.  
+
<p>8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.  
 
</p>
 
</p>
 
                                 </div>
 
                                 </div>
Line 378: Line 371:
 
                                  
 
                                  
 
                                 <div class="content">
 
                                 <div class="content">
−
                                     <p>In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOtags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.
+
                                     <p>In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOTags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.
 
</p>
 
</p>
 
                                 </div>
 
                                 </div>
Line 400: Line 393:
 
          
 
          
 
          
 
          
−
       
+
                                <!-- footer============================================================================== -->
−
       
+
−
       
+
−
       
+
−
       
+
−
        <!-- footer============================================================================== -->
+
 
         <style>
 
         <style>
 
             footer .copyright span:before {
 
             footer .copyright span:before {
Line 421: Line 409:
 
         </style>
 
         </style>
 
         <footer id="page-footer">
 
         <footer id="page-footer">
 +
<footer id="page-footer">
 +
                <div class="row,row1">
 +
                    <ul class="footer-social">
 +
                        <li class="col-md-6" id="PKU-administration" style="margin-bottom:25px;max-width:300px">
 +
                            <a href="http://dean.pku.edu.cn/pkudean/index.html"><img src="https://static.igem.org/mediawiki/2018/7/7a/T--Peking--images_PKU_Administration_logo.png"></a>
 +
                        </li>
 +
                        <li class="col-md-6" id="PKU-SLS" style="margin-bottom:25px;max-width:300px">
 +
                            <a href="http://www.bio.pku.edu.cn/"><img src="https://static.igem.org/mediawiki/2018/0/04/T--Peking--images_PKU_SLS_logo.png"></a>
 +
                        </li>
 +
                     
 +
                        <li class="col-md-6" id="PKU-CQB" style="margin-bottom:25px;max-width:300px">
 +
                            <a href="http://cqb.pku.edu.cn/en/"><img src="https://static.igem.org/mediawiki/2018/e/e1/T--Peking--images_PKU_CQB_logo.png"></a>
 +
                        </li>
 +
 +
                    </ul>
 +
                </div>
 
             <div class="row">
 
             <div class="row">
 
                 <div class="twelve columns" >
 
                 <div class="twelve columns" >
 
                     <ul class="copyright">
 
                     <ul class="copyright">
 
                         <!--<li>&copy; 2014 Sparrow</li> -->
 
                         <!--<li>&copy; 2014 Sparrow</li> -->
−
                         <li><a href="https://2018.igem.org/Team:Peking">Home</a>&nbsp;&nbsp;&nbsp;<a href="mailto:indigomad@pku.edu.cn">Contact</a></li>
+
                         <li><a href="https://2018.igem.org/Team:Peking">Home</a>&nbsp;&nbsp;&nbsp;<a href="mailto:pekingigem2018@126.com">Contact</a></li>
 
                         <span> &copy;2018 PEKING IGEM. All Rights Reserved.</span>
 
                         <span> &copy;2018 PEKING IGEM. All Rights Reserved.</span>
 
                         <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li>
 
                         <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li>
Line 432: Line 436:
 
                 <div id="go-top" style="display: block;"><a title="Back to Top" href="#">Go To Top</a></div>
 
                 <div id="go-top" style="display: block;"><a title="Back to Top" href="#">Go To Top</a></div>
 
             </div>
 
             </div>
−
        </footer> <!-- Footer End-->
+
 
−
       
+
 
−
       
+
<!-- Footer End-->
−
        <!-- Java Script======================================================================= -->
+
                       
−
        <script>window.jQuery || document.write('<script src="https://2016.igem.org/Template:Peking/Javascript/jquery_1_10_2_min?action=raw&ctype=text/javascript"><\/script>')</script>
+
                       
−
        <script type="text/javascript" src="https://2016.igem.org/Template:Peking/Javascript/jquery_migrate_1_2_1_min?action=raw&ctype=text/javascript"></script>
+
            <!-- Java Script======================================================================= -->
−
       
+
            <script>window.jQuery || document.write('<script src="https://2018.igem.org/Template:Peking/Javascript/jquery_1_10_2_min?action=raw&ctype=text/javascript"><\/script>')</script>
−
        <script src="https://2016.igem.org/Template:Peking/Javascript/jquery_flexslider?action=raw&ctype=text/javascript"></script>
+
            <script type="text/javascript" src="https://2018.igem.org/Template:Peking/Javascript/jquery_migrate_1_2_1_min?action=raw&ctype=text/javascript"></script>
−
        <script src="https://2016.igem.org/Template:Peking/Javascript/doubleaptogo?action=raw&ctype=text/javascript"></script>
+
           
−
        <script src="https://2016.igem.org/Template:Peking/Javascript/init?action=raw&ctype=text/javascript"></script>
+
            <script src="https://2018.igem.org/Template:Peking/Javascript/jquery_flexslider?action=raw&ctype=text/javascript"></script>
−
       
+
            <script src="https://2018.igem.org/Template:Peking/Javascript/doubleaptogo?action=raw&ctype=text/javascript"></script>
−
       
+
            <script src="https://2018.igem.org/Template:Peking/Javascript/init?action=raw&ctype=text/javascript"></script>
−
        <!--quotations from black: start-->
+
           
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery?action=raw&ctype=text/javascript"></script>
+
            <!--quotations from flexslider: start-->
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_sticky?action=raw&ctype=text/javascript"></script>
+
            <script src='https://2018.igem.org/Template:Peking/Javascript/modernizr_js?action=raw&ctype=text/javascript'></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_easing_1_3_pack?action=raw&ctype=text/javascript"></script>
+
                <script type='text/javascript' src='https://2018.igem.org/Template:Peking/Javascript/fjquery_polaroid?action=raw&ctype=text/javascript'></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/bootstrap_min?action=raw&ctype=text/javascript"></script>
+
                <script type='text/javascript' src='https://2018.igem.org/Template:Peking/Javascript/fjquery_easing?action=raw&ctype=text/javascript'></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_patallax_1_1_3?action=raw&ctype=text/javascript"></script>
+
                <script type='text/javascript' src='https://2018.igem.org/Template:Peking/Javascript/fjquery_transform_0_8_0_min?action=raw&ctype=text/javascript'></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/appear?action=raw&ctype=text/javascript"></script>
+
                <script type='text/javascript' src='https://2018.igem.org/Template:Peking/Javascript/fjquery_preloader?action=raw&ctype=text/javascript'></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/modernizr?action=raw&ctype=text/javascript"></script>
+
                <!--quotations from flexslider: end-->
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_prettyPhoto?action=raw&ctype=text/javascript"></script>
+
               
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/isotope?action=raw&ctype=text/javascript"></script>
+
                <!--quotations from black: start-->
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_bxslider_min?action=raw&ctype=text/javascript"></script>
+
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery?action=raw&ctype=text/javascript"></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_cycle_all?action=raw&ctype=text/javascript" ></script>
+
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_sticky?action=raw&ctype=text/javascript"></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_maximage?action=raw&ctype=text/javascript"></script>
+
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_easing_1_3_pack?action=raw&ctype=text/javascript"></script>
−
        <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/scripts?action=raw&ctype=text/javascript "></script>
+
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/bootstrap_min?action=raw&ctype=text/javascript"></script>
−
        <!--quotations from black: end-->
+
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_patallax_1_1_3?action=raw&ctype=text/javascript"></script>
−
    </body>
+
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/appear?action=raw&ctype=text/javascript"></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/modernizr?action=raw&ctype=text/javascript"></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_prettyPhoto?action=raw&ctype=text/javascript"></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/isotope?action=raw&ctype=text/javascript"></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_bxslider_min?action=raw&ctype=text/javascript"></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_cycle_all?action=raw&ctype=text/javascript" ></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/jquery_maximage?action=raw&ctype=text/javascript"></script>
 +
                <script type='text/javascript' src="https://2018.igem.org/Template:Peking/Javascript/scripts?action=raw&ctype=text/javascript "></script>
 +
                <!--quotations from black: end-->
 +
                           
 +
                           
 +
                            </body>
 
</html>
 
</html>

Latest revision as of 00:41, 18 October 2018

Safety

Peking iGEM 2018’s project is a breakthrough attempt to construct a new platform with phase separation system abbreviated to SPOT. With the SPOT system, we hope to achieve various biological functions such as a reaction hub or a sensor for plant hormone molecules. Here we will share our experiences with safety control.

Safe project design

Our SPOT contains two main applications. One is to use the SPOT as a reaction hub to regulate various biochemical reactions. We have demonstrated that if we integrated three enzymes which could synthesize β-carotene into SPOT system, the production of β-carotene in yeasts will be enhanced if phase separation happens. In the future, if we use the yeasts to manufacture β-carotene in a large scale, we can sequester the yeasts in fermenters to prevented recombinant yeasts or recombinant DNA from flowing out into the environment. The other is to use our SPOT as a sensor. We achieved that if there are ABA or Rapamycin molecules in the environment, phase separation would happen. If we want to use the sensor in reality, we can package the yeast cells into resins. It will help us to observe the SPOT formation by microscopy, and limit yeast escape. In both two reality scenes, escape of microbes is prohibited.

Safe materials

In our experiments, we chose E. coli as the chassis for molecular cloning, and S. cerevisiae as SPOT formation chassis. These two species are well known and non-pathogenic. Our bioparts are constructed by collecting segments from safe plasmid by which means we avoid using unknown segments. Those parts which have potential to cause environment problem, animal and plant disease, ecosystem changing are strictly prohibited.

Safe lab work

As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:

1.Duplicating a key to the laboratory without permission was strictly prohibited.

2.All involved participants needed to understand the experiment completely before commencing work.

3.All experimenters had to wear rubber gloves.

4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.

5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.

6.All liquid and solid waste potentially containing living organism was sterilized.

7.The entire lab was sterilized using UV-light every week.

8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.

Safe shipment

In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOTags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.