Difference between revisions of "Team:Peking/Safety"

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{{Peking}}
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                    <a class="show-menu" data-toggle="collapse" data-target=".nav-collapse"><span class="show-menu-bar"></span>
 +
                    </a>
 +
                    <div id="logo" style="max-width:0px"><a class="" href="https://2018.igem.org/Team:Peking"><img
 +
                    src="https://static.igem.org/mediawiki/2018/3/36/T--Peking--Logo.png"></a></div>
 +
                 
 +
                    <div class="nav-collapse collapse">
 +
                        <ul class="nav">                          
  
 +
                            <li class="menu-1"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking">Home</a>
 +
                            </li>
  
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<div class="column full_size">
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                            <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Project</a>
 +
                                <ul class="dropdown-menu">
 +
                                    <li><a href="https://2018.igem.org/Team:Peking/Project" class="barfont1">Description</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Peking/Perspective" class="barfont1">Perspective</a></li>
 +
                                </ul>
 +
                            </li>
 +
                            <li class="menu-3"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Model">Modeling</a>
 +
                            </li>
 +
                            <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a>
 +
                            </li>
  
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<h1> Safety </h1>
 
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
                            <li class="menu-6"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Human_Practices">Human Practices</a>
 +
                            </li>
 +
                                <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achievement</a>
 +
                                    <ul class="dropdown-menu">
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Judging_Form" class="barfont1">Judging Form</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Parts" class="barfont1">Parts</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Improve" class="barfont1">Improvement</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/InterLab" class="barfont1">InterLab</a></li>
  
−
</div>
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                                    </ul>
 +
                                </li>
 +
                                <li class="dropdown menu-8"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Lab</a>
 +
                                    <ul class="dropdown-menu">
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Team_Members">Team Members</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Attributions" class="barfont1">Attributions</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Notebook" class="barfont1">Notebook</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Peking/Acknowledgement" class="barfont1">Acknowledgement</a></li>
 +
                                       
 +
                                    </ul>
 +
                                </li>
 +
                                </div>
 +
                </div>
 +
            </div>
 +
        </div>
 +
        <!--/Navigation -->
 +
       
 +
       
 +
        <!-- Page Title======================================================================== -->
 +
        <div id="page-title">
 +
            <div class="row">
 +
                <div class="twelve columns centered text-center">
 +
                    <h1>Safety</h1>
 +
                    <p class="title1" style="text-align:justify; text-justify:inter-ideograph;">Peking iGEM 2018’s project is a breakthrough attempt to construct a new platform with phase separation system abbreviated to SPOT. With the SPOT system, we hope to achieve various biological functions such as a reaction hub or a sensor for plant hormone molecules. Here we will share our experiences with safety control.</p>
 +
                </div>
 +
            </div>
 +
        </div><!-- Page Title End-->
 +
       
 +
       
 +
        <div id="page-content" class="row page">
 +
            <div id="primary" class="twelve columns">
 +
                <section>
 +
                    <div class="row">
 +
                       
 +
                       
 +
                       
 +
                       
 +
                        <div class="three columns">
 +
                            <div id="page-wrap">
 +
                                <div id="sidebar" style="color:#000000">
 +
                                   
 +
                                    <h4><a href="https://2018.igem.org/Team:Peking/Team_Members">&bull;Team&nbsp;Members</a></h4>
 +
                                    <h4><a href="https://2018.igem.org/Team:Peking/Attributions">&bull;Attributions</a></h4>
 +
                             
 +
                                    <h4><a href="https://2018.igem.org/Team:Peking/Notebook">&bull;Notebook</a></h4>
 +
                                    <h4><a href="https://2018.igem.org/Team:Peking/Collaborations">&bull;Collaborations</a></h4>
 +
                                    <h4><a href="https://2018.igem.org/Team:Peking/Safety">&bull;Safety</a></h4>
 +
                                    <h4><a href="https://2018.igem.org/Team:Peking/Acknowledgement">&bull;Acknowledgement</a></h4>
 +
                             
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                       
 +
                       
 +
                       
 +
                       
 +
                        <div class="nine columns">
 +
                           
 +
                            <div class="texttitle">Safe project design
 +
<a id="A"></a></div>
 +
                            <hr style="border:2px dashed; height:2px" color="#666666">
  
 +
                            <div class="coll">
 +
                               
 +
                                <div class="content">
 +
                                    <p>Our SPOT contains two main applications. One is to use the SPOT as a reaction hub to regulate various biochemical reactions. We have demonstrated that if we integrated three enzymes which could synthesize β-carotene into SPOT system, the production of  β-carotene in yeasts will be enhanced if phase separation happens. In the future, if we use the yeasts to manufacture β-carotene in a large scale, we can sequester the yeasts in fermenters to prevented recombinant yeasts or recombinant DNA from flowing out into the environment. The other is to use our SPOT as a sensor. We achieved that if there are ABA or Rapamycin molecules in the environment, phase separation would happen. If we want to use the sensor in reality, we can package the yeast cells into resins. It will help us to observe the SPOT formation by microscopy, and limit yeast escape. In both two reality scenes, escape of microbes is prohibited. </p>
 +
                                </div>
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<h3>Safe Project Design</h3>
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                            <div class="texttitle">Safe materials
 +
<a id="A"></a></div>
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                            <hr style="border:2px dashed; height:2px" color="#666666">
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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                                <div class="content">
 +
                                    <p>In our experiments, we chose E. coli as the chassis for molecular cloning, and S. cerevisiae as SPOT formation chassis. These two species are well known and non-pathogenic. Our bioparts are constructed by collecting segments from safe plasmid by which means we avoid using unknown segments. Those parts which have potential to cause environment problem, animal and plant disease, ecosystem changing are strictly prohibited.</p>
 +
                                </div>
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                            </div>
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<div >
 +
                           
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                            <div class="texttitle">Safe lab work
 +
<a id="A"></a></div>
 +
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<ul>
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                            <div class="coll">
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<li>Choosing a non-pathogenic chassis</li>
+
                               
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
                                <div class="content">
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
                                    <p>As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:</p>
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
<p>1.Duplicating a key to the laboratory without permission was strictly prohibited.</p>
−
</ul>
+
<p>2.All involved participants needed to understand the experiment completely before commencing work.</p>
 +
<p>3.All experimenters had to wear rubber gloves.</p>
 +
<p>4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.</p>
 +
<p>5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.</p>
 +
<p>6.All liquid and solid waste potentially containing living organism was sterilized.</p>
 +
<p>7.The entire lab was sterilized using UV-light every week.</p>
 +
<p>8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.
 +
</p>
 +
                                </div>
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                            </div>
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                            <div >
 +
                           
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                            <div class="texttitle">Safe shipment
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<a id="A"></a></div>
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                                <div class="content">
 +
                                    <p>In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOTags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.
 +
</p>
 +
                                </div>
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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Latest revision as of 00:41, 18 October 2018

Safety

Peking iGEM 2018’s project is a breakthrough attempt to construct a new platform with phase separation system abbreviated to SPOT. With the SPOT system, we hope to achieve various biological functions such as a reaction hub or a sensor for plant hormone molecules. Here we will share our experiences with safety control.

Safe project design

Our SPOT contains two main applications. One is to use the SPOT as a reaction hub to regulate various biochemical reactions. We have demonstrated that if we integrated three enzymes which could synthesize β-carotene into SPOT system, the production of β-carotene in yeasts will be enhanced if phase separation happens. In the future, if we use the yeasts to manufacture β-carotene in a large scale, we can sequester the yeasts in fermenters to prevented recombinant yeasts or recombinant DNA from flowing out into the environment. The other is to use our SPOT as a sensor. We achieved that if there are ABA or Rapamycin molecules in the environment, phase separation would happen. If we want to use the sensor in reality, we can package the yeast cells into resins. It will help us to observe the SPOT formation by microscopy, and limit yeast escape. In both two reality scenes, escape of microbes is prohibited.

Safe materials

In our experiments, we chose E. coli as the chassis for molecular cloning, and S. cerevisiae as SPOT formation chassis. These two species are well known and non-pathogenic. Our bioparts are constructed by collecting segments from safe plasmid by which means we avoid using unknown segments. Those parts which have potential to cause environment problem, animal and plant disease, ecosystem changing are strictly prohibited.

Safe lab work

As the iGEM safety policy requires, we didn’t do any dangerous experiments in daily bench work or faced any unusual safety issues. The bench work followed some basic regulations as below:

1.Duplicating a key to the laboratory without permission was strictly prohibited.

2.All involved participants needed to understand the experiment completely before commencing work.

3.All experimenters had to wear rubber gloves.

4.Any steps involving potential release of live microorganisms were performed in a bio-safety cabinet.

5.Appliances such as Bunsen burners, electric heaters and microwave ovens were not left unattended while in use.

6.All liquid and solid waste potentially containing living organism was sterilized.

7.The entire lab was sterilized using UV-light every week.

8.The last person to leave the lab always made sure that water, electricity, gas, and the air conditioner were shut down, and doors and windows have been locked before leaving.

Safe shipment

In the process of shipment, the DNA parts are absolutely safe because they encode non-hazardous proteins like HOTags and enzymes for carotene synthesis. The DNA parts were safely confined within 96-well plate as the Parts Registry requires.