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− | {{UC_Davis}} {{Template: UC_Davis/header}}
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| <head> | | <head> |
| + | <title>Supplementary</title> |
| + | <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css"> |
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| } | | } |
| + | /*------------Get rid of iGEM stuff----------------------------------*/ |
| + | #home_logo, #sideMenu { display:none; } |
| + | #sideMenu, #top_title11, .patrollink {display:none;} |
| + | #content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;} |
| + | /*-------------------------------------------------------------------*/ |
| </style> | | </style> |
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| <body> | | <body> |
− | <div style='padding-top: 150px;'></div>
| + | <div style='padding-top: 30px;'></div> |
| <center> | | <center> |
− | <div style = ' padding-bottom: 30px; font-size: 40px; color: #d9a900'; ><b>Supplementary Material</b></div> | + | <div style = 'padding-bottom: 60px; font-size: 30px; color: #d9a900'; ><b>Supplementary Material</b></div> |
− | </center>
| + | </center> |
− | <div style='padding-top: 30px;'></div>
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− | <div style = 'padding-bottom: 22px; font-size: 25px; color: #d9a900'; ><b>I. Full FASTA Sequences</b></div>
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− | <div style='padding-top: 20px;'></div>
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− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | i. Metallothionein 1 promoter
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− | | + | |
− | </b></i>
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− | </div>
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− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>MT1_promoter_mus_musculus</b>
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− | </div>
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− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | CCACACGTCACATGGGTCGTCCTATCCGAGCCAGTCGTGCCAAAGGGGCGGTCCCGCTGT
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− | GCACACTGGCGCTCCAGGGAGCTCTGCACTCCGCCCGAAAAGTGCGCTCGGCTCTGCCA
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− | AGGACGCGGGGCGCGTGACTATGCGTGGGCTGGAGCAACCGCCTGCTGGGTGCAAACCC
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− | TTTGCGCCCGGACTCGTCCAACGACTATAAAGAGGGCAGGCTGTCCTCTAAGCGTCACCA
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− | CGACTTCAACGTCCTGAGTACCTTCTCCTCACTTACTCCGTAGCTCCAGCTTCACCAGATCT
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− | CGGAA
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− | | + | |
− | </div>
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− | | + | |
− | | + | |
− | <div style='padding-top: 20px;'></div>
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− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | ii. Metallothionein 2a full promoter
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− | </b></i>
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− | </div>
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− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>X00504.1 Human metallothionein gene IIA promoter region</b>
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− | </div>
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− | | + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
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− | ACACGGCGGAGGCGCACGGCGTGGGCACCCAGCACCCGGTACACTGTGTCCTCCCGCTG
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− | CACCCAGCCCCTTCAGCGCGAGGCGTCCCCGAGGCGCAAGTGGGCCGCCTTCAGGGAAC
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− | TGACCGCCCGCGGCCCGTGTGCAGAGCCGGGTGCGCCCGGCCCAGTGCGCGCGGCCGG
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− | GTGTTTCGCTTGGAGCCGCAAGTGACTTCTAGCGCGGGGCGTGTGCAGGGACGGCCGGG
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− | GCGGGGCTTTTGCACTCGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCAC
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− | TCCACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCG
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− | CCTCTTCAGCTCGCCATGATC
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− | | + | |
− | </div>
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− | | + | |
− | | + | |
− | | + | |
− | | + | |
− | <div style='padding-top: 20px;'></div>
| + | |
− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | iii. Metallothionein 2a minimal promoter version 1
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− | </b></i>
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− | </div>
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− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>MT2_promoter_59_bp_homo_sapiens </b>
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− | </div>
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− | | + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
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− | CGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCACTCCACCACGCCTCC
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− | </div>
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− | | + | |
− | | + | |
− | <div style='padding-top: 20px;'></div>
| + | |
− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | iv. Metallothionein 2a minimal promoter version 2
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− | </b></i>
| + | |
− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>MT2a_promoter_60_bp_first_A_added_homo_sapiens </b>
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− | </div>
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− | | + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | ACGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCACTCCACCACGCCTCC
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− | </div>
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− | | + | |
− | | + | |
− | <div style='padding-top: 20px;'></div>
| + | |
− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | v. Metallothionein 2a minimal promoter version 3
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− | </b></i>
| + | |
− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
| + | |
− | <b>MT2a_promoter_60_bp_last_A_added_homo_sapiens </b>
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− | </div>
| + | |
− | | + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | CGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCACTCCACCACGCCTCCA
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− | </div>
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− | | + | |
− | | + | |
− | <div style='padding-top: 20px;'></div>
| + | |
− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
| + | |
− | vi. CYP1A1 promoter
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− | </b></i>
| + | |
− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
| + | |
− | <b>NG_055251.1:101-1802 Mus musculus Cyp1a1 5' regulatory region (LOC110467519) on chromosome 9 </b>
| + | |
− | </div>
| + | |
− | | + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | ACTGTCCCCTGCATATCATTTATCTTTCTAGTGCTCTTTTTGTACTATGCCTGTCTCTGTGTT
| + | |
− | TTGTTACTTATCACCTGTTTCCCCCATAGCAGCGGGTAAGCTTCGTCAAAGGAGTATTGTTA
| + | |
− | ATCCTTTTCACCTCTGCATCAATCACCAGCATCCAGCATAGTAGGAGCTTGGCGAATATTTG
| + | |
− | CACAATGCATGAGGGCGGCATGTAACTAGAGTGGGAGGTGAGGGGGTGGATGAAGTGTT
| + | |
− | GTTTTTTTTTCCTGGTCTTGGTCACAGAGCAGATATCAATGTTCTTTCTGGGCTCGGGAGCT
| + | |
− | CACAGGGGAGGGCAGGTGAAGGTGTTAGTTAGGAACAGGTTGAGTTAGACACGCCAAGTT
| + | |
− | CAGATGTTCTTCTTACACTAATCTCACTCTGGGAGCACCCTTCAGGGCCAGAGAGCACCTG
| + | |
− | CAAAACAGCCAGCTAGGCGTGCCGGGTGGTGCTGCGTGTCCTGCCACTAAAAATGTGGAC
| + | |
− | ACACGCTGTTCAGGCCTTTGCTCTCAGGCAGAAGCCGAGCATCGCACGCAAACCCGGCCC
| + | |
− | TTTGCACCGCTGGCGCTGTCTTGTCGCGCCCTTGCAAAGCATAGACTATTTCTATCTCTTAA
| + | |
− | ACCCCACCCCAACGCCCCAGGAGAGCTGGCCCTTTAAGAGCCTCACCCACGGTTCCCCTC
| + | |
− | CCCCAGCTAGCGTGACAGCACTGGGACCCGCGCCCGGTTGTGAGTTGGGTAGCTGGGTG
| + | |
− | GCTGCGCGGGCCTCCAGGCTCTTCTCACGCAACTCCGGGGCACCTTGTCCCCAGCCAGG
| + | |
− | TGGGGCGGAGACAGGCAGCCCGACCTCTGCCCCCAGAGGATGGAGCAGGCTTACGCACG
| + | |
− | CTAGCCTCAGGAACCTGTGTGCGTGCCAAGCATCACCCTTTGTAGCCCCAGACCCCCTCC
| + | |
− | TGCTGTCTCGCGTGGATCCTTCCTCCACCCTTTCCTCCACCATACTTAGATAGCTCTGCAC
| + | |
− | CCGCCGCCCGACTTCTCTGTGTTGCCTGCTTCAGTATGTATGCACAACACTAGCAAGCCCC
| + | |
− | GGGAGCCTACAGGGAGCTGCAAGCGGGGACTCCCGGGCTTGCAGCTTGCCTAAGGTGAC
| + | |
− | CCTTAGAGGTGAGATCTGCAAGCCTGCCATCCATCCCCACCCTCTAGATGAAGCAGCGCG
| + | |
− | AACTTCGGCCGATACCCAATTTGTGGGGCACAGAGTCAGTCCAATGGCGCCACTGGCCTT
| + | |
− | CCTGTCCTGTGACCTCTGGGCTGGGGTCGTTGCGCTTCTCACGCGAGCTTGGACTCAGTA
| + | |
− | ATCCAGGGAAGCAAAGTCACCACCCAGCTGTTCTCCCTCTACCAGCCTTTCCCGGGCACC
| + | |
− | CATTGGCTTGTAGTAGGCAAGAGGATCTTACACACTGAATGTTTCAGGGGTGCAAAAATAG
| + | |
− | TGAAAAGGAATCCCTATGACCCGGAATGGAGGCCCCAGTACTTACTTTTTAGGTTACCCCA
| + | |
− | GAATTTTTCCTCAAACCCCTCCCTCAGTGGGATTATGCACTGTCCATGGAGCACCTTGAAA
| + | |
− | GTGTGGGGGGTGGTGACCCCAACCTTTATTCTTTTTCTTTTCTTATGTTTTTTTGTGCCTGTA
| + | |
− | CCTACATCAGGTATCCGGTATGGCTTCTTGCCTATCTCCCCCCTCCCCCCTGCCTAGAGCA
| + | |
− | CTCCCTAAGGCTGTCCCTCCCTCGGTCCCACCACTGGGCTCAGATAAGGAGGCGTGGCCA
| + | |
− | ACAGACACAGAGTCCTATAAAGGTGGTGGTGCCTTCACCCTAACCCTGAAGGTGGTAGTTC
| + | |
− | TTGGAGCTTCCCCGATCCTCCCTAGGGTCCTAGAGAACACTCTTCACTTCAGTCCCTCCTT
| + | |
− | ACAGCCCAAGGTGAGTGCTTGGGGGGGCTCCTGGATTGCCCCTCCCTCCCTCCCTCCCTC
| + | |
− | CCTCCCTCCCTCCCAAGAAAAGGA
| + | |
− | </div>
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− | | + | |
− | <div style='padding-top: 20px;'></div>
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− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | Vii. FGF21 promoter
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− | </b></i>
| + | |
− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>FGF21_promoter_homo_sapiens </b>
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− | </div>
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− | | + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | GCCACCCCTCCCCCCAGGTTATTTCAGGAGCACCTGCCTGGGCCTGGGATGGCTTCTCTG
| + | |
− | GTGAAAGAAACACCAGGATTGCATCAGGGAGGAGGAGGCTGGGATGTCCAGGGTCTGAG
| + | |
− | CATCTGAGCAGGGACAGATGAGGTTGAGGTTGGCCCACGGCCAGGTGAGAGGCTTCCAA
| + | |
− | GGCAGGATACTTGTGTCTCAGATGCGGTCGCTTCTTTCATACAGCAATTGCCGCCTTGCTG
| + | |
− | AGGATCAAGGAACCTCAGTGTCAGATCACGCCCTCCCCCCAAACTTAGAAATTCAGATGGG
| + | |
− | GCGCAGAAATTTCTCTTGTTCTGCGTGATCTGCATAGATGGTCCAAGAGGTGGTTTTTCCA
| + | |
− | GGAGCCCAGCACCCCTCCTCCCTCCGACTCAGGTGCTTGAGACCCCAGATCCTTCTCTCT
| + | |
− | GAGACTCAGGAATGTGGGCCCCCAGCCCCTTTCACCTGGGTCCCAGCTAACCCGATCCTC
| + | |
− | CCCTCCCTCATCCCCTAGACCCAGGAGTCTGGCCCTCCATTGAAAGGACCCCAGGTTACA
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− | TCATCCATTCAGGCTGCCCTTGCCACGATGGAATTCTGTAGCTCCTGCCAAATGGGTCAAA
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− | TATCATGGTTCAGGCGCAGGGAGGGTGATTGGGCGGGCCTGTCTGGGTATAAATTCTGGA
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− | GCTTCTGCATCTATCCCAAAAAACAAGGGTGTT
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− | </div>
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− | | + | |
− | | + | |
− | <div style='padding-top: 20px;'></div>
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− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | viii. GADD45α promoter
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− | </b></i>
| + | |
− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>GADD45α_promoter_homo_sapiens </b>
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− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | TTGAGAAAGGAGAATCTGGGTTGCCAGGGATTCCCACATGTGGTCACCAGCTCCCCACGC
| + | |
− | AGACCAGCTCACGATTTCCCAGTTACACCGGGCAGGTGGGAAACCGTTCTGCTTTCTGTG
| + | |
− | GAAAAGATTCTAACTTGGTTCCCTGCCATCCCTGAATACAAACGGGTTGGTTTTTCTTTTTT
| + | |
− | GAGCTTCCAACCCTTGCAGCTTTCCAAAAATAAATCAAACCAGCCATCAGGGCACCGAAAT
| + | |
− | AATACTACTGCTAATAAGCAGCTTCGCCTAGACTTAGATAAACAACACTTCTGAGGTAAACT
| + | |
− | TTGCCCCGGAGGTCTGGAGACACTTTTTTAATGTAACCTGCTTACTAATAATTACTAGACTT
| + | |
− | CAGTGCATTAACCCTGGAAATAGATTTTAATAGCCACCCCTTAAAACAAAAGACATGAAAAG
| + | |
− | ATAATAAGAAAAAAGTGCCGCAACTATTATAGAAAAACACTTGGCAGCCTGCTTCAGCCCAA
| + | |
− | GCTGAGGCCACCTCTAGCCTCTGCTAAAGCCCCCCACTCCCAATGGTCCCCGCCAACCGG
| + | |
− | ATAAGAGTGCGCGCGGGACCCGCCTTCCCCTCTCGGCACCGCCCCCGCCCCCGCCCCCT
| + | |
− | CGGCTCGCCTCCCGCGTGGCTCCTCCCTTTTCCGCTCCTCTCAACCTGACTCCAGGAGCT
| + | |
− | GGGGTCAAATTGCTGGAGCAGGCTGATTTGCATAGCCCAATGGCCAAGCTGCATGCAAAT
| + | |
− | GAGGCGGAAGGTGGTTGGCTGAGGGTTGGCAGGATAACCCCGGAGAGCGGGGCCCTTTG
| + | |
− | TCCTCCAGTGGCTGGTAGGCAGTGGCTGGGAGGCAGCGGCCCAATTAGTGTCGTGCGGC
| + | |
− | CCGTGGCGAGGCGAGGTCCGGGGAGCGAGCGAGCAAGCAAGGCGGGAGGGGTGGCCG
| + | |
− | GAGCTGCGGCGGCTGGCACAGGAGGAGGAGCCCGGGCGGGCGAGGGGCGGCCGGAGA
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− | GCGCCAGGGCCTGAGCTGCCGGAGCGGCGCCTGTGAGTGAGTGC
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− | | + | |
− | </div>
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− | | + | |
− | <div style='padding-top: 20px;'></div>
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− | <div style = 'font-size: 20px;color: #a6d4f4; padding-right: 130PX; line-height: 25px; padding-bottom: 20px;' ><b><i>
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− | ix. GADD153 promoter
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− | </b></i>
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− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; padding-bottom: 10px; line-height: 25px;' >
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− | <b>GADD153_promoter_cricetulus_griseus </b>
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− | </div>
| + | |
− | <div style = 'padding-left: 40PX; padding-right: 130PX; line-height: 25px;' >
| + | |
− | AGAACAGGAATTCTGGCGTGCAGTGGGCGACTCAGAAACGCCCAAAGGTGCTCCCCCGA
| + | |
− | GACAAGCGGTNGGACTACCATGGAAATGCCAGGACCTCAGCCTCTGGGAAGCGTTAGGAG
| + | |
− | GTTAAAAGAGATGAGATCCCTTCTAAAGGGCTGGAGAAGATGTCAGTCCAGGTAGGACTAA
| + | |
− | TGGAAACTTTATCGCGGTTCCAGGGGCCTCGGNNCGACTGAGCTGGGAGGGGCCGGGAA
| + | |
− | GCTGGGAGTCTGGATGGAGGACGAAGTTGGAGGTGATGGGAGGTGGGTGGGCAGACCG
| + | |
− | CAGCTCCTGGGCAGACAAGTTCAGGAAGGACAGCCGTTGGGGCCGTTGGATACTGGGAG
| + | |
− | CTGGCGCTCCGCCCTCTTCCCTCTCATCCCCCCACCCGCGCCTCCCACCACCGTCGGCG
| + | |
− | GCCCCTGCGCGTGCGCGCGCGCAGACACCGGTTGCCAAACATTGCATCATCCCCGCCCC
| + | |
− | CCGTCATCCCTCCCTCGCCGCACTCTCCTTCGCCCGCCCGCGCGCGCGCGCGCGCGCGC
| + | |
− | GNGCGCATGACTCACTCACCTCCTCCGCGGAGCCTCGTGACCCAAAGCCACTTCCGGGTC
| + | |
− | CAAGACAACGTAGCTCTCCAGCCAGAGGGCGGGGCGGAGGCGGGGGCCGAGGGGGCTC
| + | |
− | CTGAGTGGCGGATGTAGGGGTGGGGCGGAGTCAGTGCCAGCGTGCCGCTTTCTGATTGG
| + | |
− | CAGGCTCCTGGGTCCCGCCCCCCAAAAGAGGGGACGGGCCCGCATAAAATATCTTCTCTC
| + | |
− | GGCGCTGCAGAGGTCAGTAGCCTGAGTCTCACACTTCGGTC
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− | | + | |
− | </div>
| + | |
− | | + | |
| | | |
| + | <div style = 'padding-left:7%;padding-bottom: 22px; font-size: 25px; color: #667d9d'; ><b>Supplementary Material</b></div> |
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| + | <center> |
| + | <embed src= " https://static.igem.org/mediawiki/2018/2/2a/T--UC_Davis--newfastaandprimers.pdf" width= "600" height= "400" style ='border-width: 3px;border-color: gray;'> |
| + | </center> |
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| + | <div style='padding-top: 100px;'></div> |
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− | <div style='padding-top: 100px;'></div>
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