Difference between revisions of "Team:Newcastle/Protocols"

 
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                 <h3>Experiments</h3>
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                         Our Project
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                         Overview
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                        Contact Us
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                    <span>Scroll Down</span>
 
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                <a href="https://twitter.com/newcastle_igem?lang=en"><i class="fa fa-twitter" aria-hidden="true"></i><span>Twitter</span></a>
 
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                <a href="https://www.instagram.com/newcastle_igem/?hl=en"><i class="fa fa-instagram" aria-hidden="true"></i><span>Instagram</span></a>
 
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                <h3 class="subhead subhead--dark">Hello There</h3>
 
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                <h1 class="display-1 display-1--light">We are Alternative Roots</h1>
 
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                <p class="about-para">Sustainability is a topic of increasing concern in the fields of agriculture, food security and rural development. There is a dire need for innovation in this field; primarily driven by predictions of substantial global population increase coupled with severe pressure on non-renewable resources. The result is a necessity to increase food production whilst reducing our impact on the environment. As such, our aim is to find sustainable solutions that address some of these issues. </p>
 
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                <p class="about-para">Nitrogen-fixation for fertilizer production is extremely energy-intensive, accounting for 80% of energy use in agriculture. This is due to the high temperatures and pressures involved in the Haber-Bosch process. Nitrogen is essential for plant growth but cannot be directly accessed from the atmosphere by plants despite its abundance. If an alternative to fertilizers could be developed to provide nitrogen for plant growth that is cheap, easy to use and sustainable, then energy use in the agriculture sector could be greatly reduced.</p>
 
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<p class="about-para">In 2018, Team Newcastle aim to engineer microbes for sustainable agriculture. The team shall build upon Newcastle University’s long and illustrious history in agriculture and food security research by engineering root colonising microbes. The microbes will attract bacteria that modify the soil's composition including nitrogen content, in a fashion that is suitable for uptake by plants via the roots.</p>
 
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                <div class="stats__count">10</div>
 
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                <h5>BILLION PEOPLE WILL</h5>
 
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                <h5>INHABIT EARTH BY 2050</h5>
 
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            </div>
 
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            <div class="col-block stats__col">
 
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                <div class="stats__count">83</div>
 
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                <h5>MILLION EXTRA PEOPLE</h5>
 
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                <h5>NEED TO BE FED EACH YEAR</h5>
 
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            </div>
 
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            <div class="col-block stats__col">
 
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                <div class="stats__count">842</div>
 
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                <h5>MILLION PEOPLE SUFFER</h5>
 
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                <h5>FROM HUNGER WORLDWIDE</h5>
 
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            <div class="col-block stats__col">
 
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                <div class="stats__count">200</div>
 
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                <h5>MILLION TONNES OF</h5>
 
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                <h5>FERTILIZER USED ANNUALLY</h5>
 
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     <section id='protocol' class="s-services">
  
 
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                             <h3 class="subhead">2018</h3>
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                             <h3 class="subhead">EXPERIMENTS</h3>
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                 <h1 class="display-2">The Newcastle Team</h1>
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                 <h1 class="display-2">PROTOCOLS</h1>
 
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                    <a href="https://2018.igem.org/Team:Newcastle/Team" onclick="location.href='https://2018.igem.org/Team:Newcastle/Team'"><font face="verdana" color="green">MEET FULL TEAM</font> </a>             
 
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<table id="protocols">
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      <thead>
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          <th>Protocol</th>
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                <div class="service-text">
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          <th>Author</th>
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                    <h3 class="h2">Luke Waller</h3>
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          <th>Category</th>
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                    <img src="https://static.igem.org/mediawiki/2018/b/bc/T--Newcastle--Luke.png">
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          <th>PDF File</th>
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                    <p><br> Luke’s my name, Conner is my game. I am the engineering part Conner wants to intertwine with. Having finished my
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        </tr>
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                    third year I am staying on at Newcastle to complete my masters, hopefully getting into robotics.  
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      </thead>
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I took this role as I wanted to explore an avenue of engineering I was not familiar with and work with a multi-disciplined team. </p>
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      <tbody>
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        <tr>
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          <td>Glycerol Stock Preserves</td>
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                </div>
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          <td>Connor Trotter</td>
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            </div>
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          <td>General</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/5/5a/T--Newcastle--GlycerolStock1.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
        <tr>
 +
          <td>Bacterial Isolation from Freeze-Dried Pellet</td>
 +
          <td>Connor Trotter</td>
 +
          <td>General</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/7/72/T--Newcastle--BacIsolation1.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
        <tr>
 +
          <td>Cell Counting</td>
 +
          <td>Connor Trotter</td>
 +
          <td>Chemotaxis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/8/89/T--Newcastle--Haemocytometer1.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
<tr>
 +
        <td>Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide</td>
 +
        <td>Connor Trotter</td>
 +
        <td>Chemotaxis</td>
 +
        <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--ibidiprotocol.pdf" style="color:green">View<a/></td>
 +
        </tr>
 +
        <tr>
 +
          <td>Minimal A Salt Agar Chemotaxis Assay</td>
 +
          <td>Connor Trotter</td>
 +
          <td>Chemotaxis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/7/7f/T--Newcastle--MinAgar.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
       
 +
        <tr>
 +
        <td>0.75 % Agar Chemotaxis Assay</td>
 +
        <td>Connor Trotter</td>
 +
        <td>Chemotaxis</td>
 +
        <td><a href="https://static.igem.org/mediawiki/2018/4/47/T--Newcastle--0.5ProtocolRedone.pdf" style="color:green">View<a/></td>
 +
        </tr>
 +
        <tr>
 +
        <td>0.50 % Agar Chemotaxis Assay</td>
 +
        <td>Connor Trotter</td>
 +
        <td>Chemotaxis</td>
 +
        <td><a href="https://static.igem.org/mediawiki/2018/2/2a/T--Newcastle--0.5%25ProtocolRedoneTrue.pdf" style="color:green">View<a/></td>
 +
        </tr>
 +
        <tr>
 +
        <td>Growth Curve in Liquid Culture</td>
 +
          <td>Sadiya Quazi</td>
 +
          <td>Chemotaxis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/6/6a/T--Newcastle--GCIL.pdf" style="color:green">View</a></td>
 +
        </tr>
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        <tr>
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        <tr>
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        <td>Quantitative Capillary Assay</td>
 +
          <td>Connor Trotter</td>
 +
          <td>Chemotaxis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/a/a1/T--Newcastle--CapillaryAssayChemo.pdf" style="color:green">View</a></td>
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        </tr>
  
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        <tr>
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                <div class="service-icon">
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          <td>Naringenin Kill Curve in Liquid Culture</td>
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                    <i class="icon-group"></i>
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          <td>Sadiya Quazi</td>
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                </div>
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          <td>Chemotaxis</td>
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                <div class="service-text">
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          <td><a href="https://static.igem.org/mediawiki/2018/7/76/T--Newcastle--NarKillCurves.pdf" style="color:green">View</a></td>
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                    <h3 class="h2">Frank Eardley</h3>
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        </tr>
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                    <img src="https://static.igem.org/mediawiki/2018/0/04/T--Newcastle--Frank.png">
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        <tr>
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                    <p><br>I am a 4th year MBiol Cellular & Molecular Biology student. I joined iGEM
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          <td>Planting Seeds in Agar-Filled Microcentrifuge Tubes</td>
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                    for the opportunity to work with students from other academic fields and learn
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          <td>Lewis Tomlinson</td>
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                    more about synthetic biology. Outside of my degree, I am a scuba diving instructor
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          <td>Root Colonisation</td>
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                    and enjoy jumping in the North Sea whatever the weather.</p>
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          <td><a href="https://static.igem.org/mediawiki/2018/e/e3/T--Newcastle--MCPlant.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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                </div>
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          <td>Preparation and Staining of Microscopy Samples</td>
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            </div>
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          <td>Lewis Tomlinson</td>
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          <td>Root Colonisation</td>
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            <div class="col-block service-item" data-aos="fade-up">
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          <td><a href="https://static.igem.org/mediawiki/2018/b/bd/T--Newcastle--MScopy.pdf" style="color:green">View</a></td>
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                <div class="service-icon">
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        </tr>
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                    <i class="icon-megaphone"></i>
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        <tr>
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                </div>
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          <td>Planting Seeds in Agar-Filled Pipette-Tip Racks</td>
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                <div class="service-text">
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          <td>Lewis Tomlinson</td>
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                    <h3 class="h2">Will Tankard</h3>
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          <td>Root Colonisation</td>
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                    <img src="https://static.igem.org/mediawiki/2018/0/00/T--Newcastle--Will.png">
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          <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--RCK.pdf" style="color:green">View</a></td>
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                    <p><br>I’ve just completed my second year as an Architecture student. iGEM was a step
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        </tr>
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                    into the dark for me, the last time I did anything biology related was during my GCSE’s.  
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        <tr>
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                    I’m interested how the architecture and biology disciplines can merge. Synthetic Biology
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          <td>Re-isolating Endophytes</td>
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                    offers a new perspective on design starting with a bottom up approach.</p>
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          <td>Lewis Tomlinson</td>
−
                   
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          <td>Root Colonisation</td>
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+
          <td><a href="https://static.igem.org/mediawiki/2018/e/ef/T--Newcastle--RIso.pdf" style="color:green">View</a></td>
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                </div>
+
        </tr>
−
            </div>
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        <tr>
−
 
+
          <td>Electroporation of <I>Pseudomonas</I> sp.</td>
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            <div class="col-block service-item" data-aos="fade-up">
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          <td>Frank Eardley</td>
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                <div class="service-icon">
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          <td>Endophytic Chassis Development</td>
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                    <i class="icon-earth"></i>
+
          <td><a href="https://static.igem.org/mediawiki/2018/b/bc/T--NEWCASTLE--Electroporation.pdf" style="color:green">View</a></td>
−
                </div>
+
        </tr>
−
                <div class="service-text">
+
        <tr>
−
                    <h3 class="h2">Heather Bottomley</h3>
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          <td>MIC Opentrons Code (2 Plates)</td>
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                    <img src="https://static.igem.org/mediawiki/2018/8/81/T--Newcastle--Heather.png">
+
          <td>Frank Eardley</td>
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                    <p><br> Heather's the name, not doing the wiki is my game. I've just finished my                  third year of MBiol Cellular and Molecular Biology. I joined iGEM as an opportunity to formulate
+
          <td>Endophytic Chassis Development</td>
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                    a novel idea and work in a multi - disciplinary team enhancing my biological knowledge.</p>
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          <td><a href="https://static.igem.org/mediawiki/2018/a/af/T--Newcastle--KillCurveCodes.pdf" style="color:green">View</a></td>
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+
        </tr>
−
                   
+
        <tr>
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                </div>
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          <td>MIC Protocol</td>
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            </div>
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          <td>Frank Eardley</td>
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          <td>Endophytic Chassis Development</td>
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            <div class="col-block service-item" data-aos="fade-up">
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          <td><a href="https://static.igem.org/mediawiki/2018/3/39/T--Newcastle--MICProtocol.pdf" style="color:green">View</a></td>
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                <div class="service-icon">
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        </tr>
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                    <i class="icon-cube"></i>
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        <tr>
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                </div>
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          <td>MIC Plate Layout</td>
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                <div class="service-text">
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          <td>Frank Eardley</td>
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                    <h3 class="h2">Connor Trotter</h3>
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          <td>Endophytic Chassis Development</td>
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                    <img src="https://static.igem.org/mediawiki/2018/d/dc/T--Newcastle--Connor.png">
+
          <td><a href="https://static.igem.org/mediawiki/2018/b/bb/T--Newcastle--MICPlateLayout.pdf" style="color:green">View</a></td>
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                    <p><br> Connor is the name, getting my name misspelled is the game. I'm currently entering my third year
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        </tr>
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                    of Biology which has, so far, been fuelled by an excess of caffeine and binge watching a lot
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        <tr>
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                    of Rupaul's Drag Race. I joined Newcastle's team to apply what I have learned in a unique fashion by intertwining elements of Engineering and Architecture into biological concepts.</p>
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          <td>2-Part Gibson Assembly</td>
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          <td>Frank Eardley</td>
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                </div>
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          <td>Endophytic Chassis Development</td>
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            </div>
+
          <td><a href="https://static.igem.org/mediawiki/2018/7/78/T--Newcastle--2PartGibson.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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                <div class="service-icon"><i class="icon-lego-block"></i></div>
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          <td>Amplification of gBlocks and Gradient PCR</td>
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                <div class="service-text">
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          <td>Heather Bottomley</td>
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                    <h3 class="h2">Patrycja Ubysz</h3>
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          <td>Naringenin Operon Biosynthesis</td>
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                    <img src="https://static.igem.org/mediawiki/2018/3/30/T--Newcastle--Patrycja.png">
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          <td><a href="https://static.igem.org/mediawiki/2018/8/87/T--Newcastle--GBAmp.pdf" style="color:green">View</a></td>
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                    <p><br>I'm Pat, no game with my name. Just finished first year of Chemistry, I
+
        </tr>
−
                    probably liked it too much. I believe being interdisciplinary is crucial for research, especially
+
        <tr>
−
                    in Synthetic Biology. I want to contribute to the team using my chemical/physical approach. I
+
          <td>Colony PCR of Transformed DH5α Cells</td>
−
                    love working in the lab, however, would be happier without microbes. Basic coffee addict, bad
+
          <td>Heather Bottomley</td>
−
                    experience with BBQ parties. Probably follow every single NASA team account on Twitter.</p>
+
          <td>Naringenin Operon Biosynthesis</td>
−
                   
+
          <td><a href="https://static.igem.org/mediawiki/2018/a/a9/T--Newcastle--PCTransform.pdf" style="color:green">View</a></td>
−
                </div>
+
        </tr>
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            </div>
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        <tr>
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                <div class="col-block service-item" data-aos="fade-up">
+
          <td>DpnI Digestion and Purification</td>
−
                <div class="service-icon"><i class="icon-lego-block"></i></div>
+
          <td>Heather Bottomley</td>
−
                <div class="service-text">
+
          <td>Naringenin Operon Biosynthesis</td>
−
                    <h3 class="h2">Lewis Tomlinson</h3>
+
          <td><a href="https://static.igem.org/mediawiki/2018/b/b0/T--Newcastle--DPN1Diges.pdf" style="color:green">View</a></td>
−
                    <img src="https://static.igem.org/mediawiki/2018/0/0e/T--Newcastle--Lewis.png">
+
        </tr>
−
                    <p><br>Name: Lewis. Likes: llamas, guinea pigs, rock hyrax, all other animals, molecular biology, dungeons and dragons, science fiction and fantasy.
+
        <tr>
−
                    Life goal: llama sanctuary (including birds and rodents).</p>
+
          <td>Gel Extraction and Purification of the gBlocks</td>
−
                   
+
          <td>Heather Bottomley</td>
−
                </div>
+
          <td>Naringenin Operon Biosynthesis</td>
−
            </div>
+
          <td><a href="https://static.igem.org/mediawiki/2018/c/cc/T--Newcastle--GBlockGel.pdf" style="color:green">View</a></td>
−
           
+
        </tr>
−
                <div class="col-block service-item" data-aos="fade-up">
+
        <tr>
−
                <div class="service-icon"><i class="icon-lego-block"></i></div>
+
          <td>Gibson Assembly of gBlocks and pSB1C3 Backbone</td>
−
                <div class="service-text">
+
          <td>Heather Bottomley</td>
−
                    <h3 class="h2">Sadiya Quazi</h3>
+
          <td>Naringenin Operon Biosynthesis</td>
−
                    <img src="https://static.igem.org/mediawiki/2018/f/f2/T--Newcastle--Sadiya.png">
+
          <td><a href="https://static.igem.org/mediawiki/2018/1/15/T--Newcastle--Gib.pdf" style="color:green">View</a></td>
−
                    <p><br>Sadiya’s the name, which most can’t pronounce (despite it being totally phonetic). Just completed first year of Chemistry after switching from Fine Art,
+
        </tr>
−
                    and yes, it’s been a huge change, as people always exclaim when I tell them. Both benefit from having that alternative perspective and this is what drew me to
+
        <tr>
−
                      iGEM - working as part of a very cross-disciplinary team.</p>
+
          <td>Making Chemically Competent Cells</td>
−
                   
+
          <td>Heather Bottomley</td>
−
                </div>
+
          <td>Naringenin Operon Biosynthesis</td>
−
            </div>
+
          <td><a href="https://static.igem.org/mediawiki/2018/a/a6/T--Newcastle--CCell.pdf" style="color:green">View</a></td>
−
               
+
        </tr>
−
                <div class="col-block service-item" data-aos="fade-up">
+
        <tr>
−
                <div class="service-icon"><i class="icon-lego-block"></i></div>
+
          <td>Miniprep of Transformed Colonies</td>
−
                <div class="service-text">
+
          <td>Heather Bottomley</td>
−
                    <h3 class="h2">Umar Farooq</h3>
+
          <td>Naringenin Operon Biosynthesis</td>
−
                    <img src="https://static.igem.org/mediawiki/2018/8/84/T--Newcastle--Umar.png">
+
          <td><a href="https://static.igem.org/mediawiki/2018/0/02/T--Newcastle--MinPre.pdf" style="color:green">View</a></td>
−
                    <p><br>I'm Umar, a 1st year undergraduate studying Automation and Control Engineering. I joined IGEM for the
+
        </tr>
−
                    opportunity to work alongside a multi-disciplinary team and develop my skills outside of engineering. When
+
        <tr>
−
                    I'm not working on the project, I like swimming, playing the guitar and eating cake. Addicted to bad
+
          <td>Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR</td>
−
                    jokes, allergic to onions.
+
          <td>Heather Bottomley</td>
−
                    </p>
+
          <td>Naringenin Operon Biosynthesis</td>
−
                   
+
          <td><a href="https://static.igem.org/mediawiki/2018/5/5f/T--Newcastle--PCReaction.pdf" style="color:green">View</a></td>
−
                </div>
+
        </tr>
−
            </div>
+
        <tr>
−
           
+
          <td>Quantifying DNA Concentration</td>
−
                <div class="col-block service-item" data-aos="fade-up">
+
          <td>Heather Bottomley</td>
−
                <div class="service-icon"><i class="icon-lego-block"></i></div>
+
          <td>Naringenin Operon Biosynthesis</td>
−
                <div class="service-text">
+
          <td><a href="https://static.igem.org/mediawiki/2018/0/03/T--Newcastle--QuantifyingDNA.pdf" style="color:green">View</a></td>
−
                    <h3 class="h2">Chris Carty</h3>
+
        </tr>
−
                    <img src="https://static.igem.org/mediawiki/2018/e/e4/T--Newcastle--Chris.png">
+
        <tr>
−
                    <p><br>I’m Chris, 2nd year Architecture student, discovered iGEM when beginning my dissertation in the area  of Bio-Materialism. I want to take organisms that have been designed at the genetic scale and design them for use at the human scale - aiding the transition from biologically inspired to biologically engineered design. Better at architecture than Will.</p>
+
          <td>Resuspending gBlocks and Primers</td>
−
                   
+
          <td>Heather Bottomley</td>
−
                </div>
+
          <td>Naringenin Operon Biosynthesis</td>
−
            </div> -->
+
          <td><a href="https://static.igem.org/mediawiki/2018/a/a0/T--Newcastle--GBlockFragmnt.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
        <tr>
 +
          <td>SOC Media Preparation</td>
 +
          <td>Heather Bottomley</td>
 +
          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/4/4e/T--Newcastle--SOC1.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
        <tr>
 +
          <td>Transformation of the Gibson assembly product into pSB1C3</td>
 +
          <td>Heather Bottomley</td>
 +
          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/1/15/T--Newcastle--GBTransform.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
      </tbody>
 +
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                <h3 class="subhead">Our Sponsors</h3>
 
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                <h1 class="display-2">Newcastle iGEM is proud to be sponsored by:</h1>
 
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                    <a href="https://seedcell.co.uk/" title="" class="clients__slide"><img src="https://static.igem.org/mediawiki/2018/thumb/8/86/T--Newcastle--SeedCellSquare2.png/600px-T--Newcastle--SeedCellSquare2.png" /></a>
 
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                        <p>The iGEM Foundation is an independent, non-profit organization dedicated to the advancement of synthetic biology, education and competition,
 
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                        and the development of an open community and collaboration. This is done by fostering an open, cooperative community and friendly competition.</p>
 
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                        <img src="https://static.igem.org/mediawiki/2018/thumb/b/bb/T--Newcastle--iGEMGrey.png/600px-T--Newcastle--iGEMGrey.png" alt="Author image" class="testimonials__avatar">
 
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                        <p>iGEMers are building a better world by solving problems with the help of synthetic biology.
 
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                        We inspire responsible innovation through our efforts in biosafety, biosecurity and public outreach.</p>
 
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                        <img src="https://static.igem.org/mediawiki/2018/thumb/b/bb/T--Newcastle--iGEMGrey.png/600px-T--Newcastle--iGEMGrey.png" alt="Author image" class="testimonials__avatar">
 
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                        <p> This global network is leading the field, taking what they learned in the competition and expanding it to continue to build a better world.</p>
 
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                        <img src="https://static.igem.org/mediawiki/2018/thumb/b/bb/T--Newcastle--iGEMGrey.png/600px-T--Newcastle--iGEMGrey.png" alt="Author image" class="testimonials__avatar">
 
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                <h1 class="display-2 display-2--light">Reach out for a collaboration or just say hello</h1>
 
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                <h3 class="h6">Get Involved</h3>
 
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                <p><br>We are always happy to hear from individuals, groups or organisations that would like to support our project. If you wish to discuss a collaboration, sponsorship or if you just want to learn more about the project, please get in touch using the contact details on the right. Alternatively, if you would like to donate a small amount to fund our project, you can do so through <a class="link" href="https://experiment.com/projects/genetically-engineered-microbes-for-sustainable-agriculture" target="_blank">Experiment.com</a> - an online platform for discovering, funding, and sharing scientific research.
 
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<br><br>
 
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Any support is greatly appreciated and funds raised will be used in all areas of the iGEM project - such as lab equipment, human practices research, merchandise and travel to jamboree in Boston, USA.</p>
 
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                        <p>
 
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                            Devonshire Building<br>
 
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                            Newcastle University<br>
 
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                            Newcastle upon Tyne<br>
 
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                            NE1 7RU
 
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                        <h5>Email Us At</h5>
 
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                            igem.team@newcastle.ac.uk
 
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                <h1 class="display-2">References & Attributions</h1>
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<p class="about-para"><font size="2"><strong>Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley
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Latest revision as of 00:50, 18 October 2018

Alternative Roots/Protocols

Alternative Roots

Protocols

Overview

EXPERIMENTS

PROTOCOLS

Protocol Author Category PDF File
Glycerol Stock Preserves Connor Trotter General View
Bacterial Isolation from Freeze-Dried Pellet Connor Trotter General View
Cell Counting Connor Trotter Chemotaxis View
Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide Connor Trotter Chemotaxis View
Minimal A Salt Agar Chemotaxis Assay Connor Trotter Chemotaxis View
0.75 % Agar Chemotaxis Assay Connor Trotter Chemotaxis View
0.50 % Agar Chemotaxis Assay Connor Trotter Chemotaxis View
Growth Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Quantitative Capillary Assay Connor Trotter Chemotaxis View
Naringenin Kill Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Planting Seeds in Agar-Filled Microcentrifuge Tubes Lewis Tomlinson Root Colonisation View
Preparation and Staining of Microscopy Samples Lewis Tomlinson Root Colonisation View
Planting Seeds in Agar-Filled Pipette-Tip Racks Lewis Tomlinson Root Colonisation View
Re-isolating Endophytes Lewis Tomlinson Root Colonisation View
Electroporation of Pseudomonas sp. Frank Eardley Endophytic Chassis Development View
MIC Opentrons Code (2 Plates) Frank Eardley Endophytic Chassis Development View
MIC Protocol Frank Eardley Endophytic Chassis Development View
MIC Plate Layout Frank Eardley Endophytic Chassis Development View
2-Part Gibson Assembly Frank Eardley Endophytic Chassis Development View
Amplification of gBlocks and Gradient PCR Heather Bottomley Naringenin Operon Biosynthesis View
Colony PCR of Transformed DH5α Cells Heather Bottomley Naringenin Operon Biosynthesis View
DpnI Digestion and Purification Heather Bottomley Naringenin Operon Biosynthesis View
Gel Extraction and Purification of the gBlocks Heather Bottomley Naringenin Operon Biosynthesis View
Gibson Assembly of gBlocks and pSB1C3 Backbone Heather Bottomley Naringenin Operon Biosynthesis View
Making Chemically Competent Cells Heather Bottomley Naringenin Operon Biosynthesis View
Miniprep of Transformed Colonies Heather Bottomley Naringenin Operon Biosynthesis View
Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR Heather Bottomley Naringenin Operon Biosynthesis View
Quantifying DNA Concentration Heather Bottomley Naringenin Operon Biosynthesis View
Resuspending gBlocks and Primers Heather Bottomley Naringenin Operon Biosynthesis View
SOC Media Preparation Heather Bottomley Naringenin Operon Biosynthesis View
Transformation of the Gibson assembly product into pSB1C3 Heather Bottomley Naringenin Operon Biosynthesis View




References & Attributions

Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley