Difference between revisions of "Team:Newcastle/Protocols"

 
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                 <h3>Experiments</h3>
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                 <h3>Alternative Roots</h3>
  
 
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                             <h3 class="subhead">Newcastle iGEM 2018</h3>
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                             <h3 class="subhead">EXPERIMENTS</h3>
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                 <h1 class="display-2">Protocols</h1>
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                 <h1 class="display-2">PROTOCOLS</h1>
 
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           <td>Glycerol Stock Preserves</td>
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           <td>Lorem Ipsum</td>
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           <td>Connor Trotter</td>
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           <td>Lorem Ipsum</td>
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           <td>General</td>
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           <td>View</td>
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           <td><a href="https://static.igem.org/mediawiki/2018/5/5a/T--Newcastle--GlycerolStock1.pdf" style="color:green">View</a></td>
 
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           <td>Lorem Ipsum</td>
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           <td>Bacterial Isolation from Freeze-Dried Pellet</td>
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           <td>Lorem Ipsum</td>
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           <td>Connor Trotter</td>
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           <td>Lorem Ipsum</td>
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           <td>General</td>
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           <td>View</td>
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           <td><a href="https://static.igem.org/mediawiki/2018/7/72/T--Newcastle--BacIsolation1.pdf" style="color:green">View</a></td>
 
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           <td>Lorem Ipsum</td>
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           <td>Cell Counting</td>
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           <td>Lorem Ipsum</td>
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           <td>Connor Trotter</td>
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           <td>Lorem Ipsum</td>
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           <td>Chemotaxis</td>
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           <td>View</td>
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           <td><a href="https://static.igem.org/mediawiki/2018/8/89/T--Newcastle--Haemocytometer1.pdf" style="color:green">View</a></td>
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        </tr>
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<tr>
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        <td>Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide</td>
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        <td>Connor Trotter</td>
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        <td>Chemotaxis</td>
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        <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--ibidiprotocol.pdf" style="color:green">View<a/></td>
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        </tr>
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        <tr>
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          <td>Minimal A Salt Agar Chemotaxis Assay</td>
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          <td>Connor Trotter</td>
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          <td>Chemotaxis</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/7/7f/T--Newcastle--MinAgar.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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        <td>0.75 % Agar Chemotaxis Assay</td>
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        <td>Connor Trotter</td>
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        <td>Chemotaxis</td>
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        <td><a href="https://static.igem.org/mediawiki/2018/4/47/T--Newcastle--0.5ProtocolRedone.pdf" style="color:green">View<a/></td>
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        </tr>
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        <tr>
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        <td>0.50 % Agar Chemotaxis Assay</td>
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        <td>Connor Trotter</td>
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        <td>Chemotaxis</td>
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        <td><a href="https://static.igem.org/mediawiki/2018/2/2a/T--Newcastle--0.5%25ProtocolRedoneTrue.pdf" style="color:green">View<a/></td>
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        </tr>
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        <tr>
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        <td>Growth Curve in Liquid Culture</td>
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          <td>Sadiya Quazi</td>
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          <td>Chemotaxis</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/6/6a/T--Newcastle--GCIL.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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        <tr>
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        <td>Quantitative Capillary Assay</td>
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          <td>Connor Trotter</td>
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          <td>Chemotaxis</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/a/a1/T--Newcastle--CapillaryAssayChemo.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Naringenin Kill Curve in Liquid Culture</td>
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          <td>Sadiya Quazi</td>
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          <td>Chemotaxis</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/7/76/T--Newcastle--NarKillCurves.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Planting Seeds in Agar-Filled Microcentrifuge Tubes</td>
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          <td>Lewis Tomlinson</td>
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          <td>Root Colonisation</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/e/e3/T--Newcastle--MCPlant.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Preparation and Staining of Microscopy Samples</td>
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          <td>Lewis Tomlinson</td>
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          <td>Root Colonisation</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/b/bd/T--Newcastle--MScopy.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Planting Seeds in Agar-Filled Pipette-Tip Racks</td>
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          <td>Lewis Tomlinson</td>
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          <td>Root Colonisation</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--RCK.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Re-isolating Endophytes</td>
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          <td>Lewis Tomlinson</td>
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          <td>Root Colonisation</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/e/ef/T--Newcastle--RIso.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Electroporation of <I>Pseudomonas</I> sp.</td>
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          <td>Frank Eardley</td>
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          <td>Endophytic Chassis Development</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/b/bc/T--NEWCASTLE--Electroporation.pdf" style="color:green">View</a></td>
 +
        </tr>
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        <tr>
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          <td>MIC Opentrons Code (2 Plates)</td>
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          <td>Frank Eardley</td>
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          <td>Endophytic Chassis Development</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/a/af/T--Newcastle--KillCurveCodes.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>MIC Protocol</td>
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          <td>Frank Eardley</td>
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          <td>Endophytic Chassis Development</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/3/39/T--Newcastle--MICProtocol.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>MIC Plate Layout</td>
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          <td>Frank Eardley</td>
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          <td>Endophytic Chassis Development</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/b/bb/T--Newcastle--MICPlateLayout.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>2-Part Gibson Assembly</td>
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          <td>Frank Eardley</td>
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          <td>Endophytic Chassis Development</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/7/78/T--Newcastle--2PartGibson.pdf" style="color:green">View</a></td>
 +
        </tr>
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        <tr>
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          <td>Amplification of gBlocks and Gradient PCR</td>
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          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/8/87/T--Newcastle--GBAmp.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Colony PCR of Transformed DH5α Cells</td>
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          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/a/a9/T--Newcastle--PCTransform.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>DpnI Digestion and Purification</td>
 +
          <td>Heather Bottomley</td>
 +
          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/b/b0/T--Newcastle--DPN1Diges.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Gel Extraction and Purification of the gBlocks</td>
 +
          <td>Heather Bottomley</td>
 +
          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/c/cc/T--Newcastle--GBlockGel.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Gibson Assembly of gBlocks and pSB1C3 Backbone</td>
 +
          <td>Heather Bottomley</td>
 +
          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/1/15/T--Newcastle--Gib.pdf" style="color:green">View</a></td>
 +
        </tr>
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        <tr>
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          <td>Making Chemically Competent Cells</td>
 +
          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/a/a6/T--Newcastle--CCell.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Miniprep of Transformed Colonies</td>
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          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/0/02/T--Newcastle--MinPre.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR</td>
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          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/5/5f/T--Newcastle--PCReaction.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Quantifying DNA Concentration</td>
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          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/0/03/T--Newcastle--QuantifyingDNA.pdf" style="color:green">View</a></td>
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        </tr>
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        <tr>
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          <td>Resuspending gBlocks and Primers</td>
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          <td>Heather Bottomley</td>
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          <td>Naringenin Operon Biosynthesis</td>
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          <td><a href="https://static.igem.org/mediawiki/2018/a/a0/T--Newcastle--GBlockFragmnt.pdf" style="color:green">View</a></td>
 
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           <td><a href="https://static.igem.org/mediawiki/2018/4/4e/T--Newcastle--SOC1.pdf" style="color:green">View</a></td>
 
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           <td>Transformation of the Gibson assembly product into pSB1C3</td>
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           <td><a href="https://static.igem.org/mediawiki/2018/1/15/T--Newcastle--GBTransform.pdf" style="color:green">View</a></td>
 
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                <h1 class="display-2">References & Attributions</h1>
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<p class="about-para"><font size="2"><strong>Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley
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Latest revision as of 00:50, 18 October 2018

Alternative Roots/Protocols

Alternative Roots

Protocols

Overview

EXPERIMENTS

PROTOCOLS

Protocol Author Category PDF File
Glycerol Stock Preserves Connor Trotter General View
Bacterial Isolation from Freeze-Dried Pellet Connor Trotter General View
Cell Counting Connor Trotter Chemotaxis View
Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide Connor Trotter Chemotaxis View
Minimal A Salt Agar Chemotaxis Assay Connor Trotter Chemotaxis View
0.75 % Agar Chemotaxis Assay Connor Trotter Chemotaxis View
0.50 % Agar Chemotaxis Assay Connor Trotter Chemotaxis View
Growth Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Quantitative Capillary Assay Connor Trotter Chemotaxis View
Naringenin Kill Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Planting Seeds in Agar-Filled Microcentrifuge Tubes Lewis Tomlinson Root Colonisation View
Preparation and Staining of Microscopy Samples Lewis Tomlinson Root Colonisation View
Planting Seeds in Agar-Filled Pipette-Tip Racks Lewis Tomlinson Root Colonisation View
Re-isolating Endophytes Lewis Tomlinson Root Colonisation View
Electroporation of Pseudomonas sp. Frank Eardley Endophytic Chassis Development View
MIC Opentrons Code (2 Plates) Frank Eardley Endophytic Chassis Development View
MIC Protocol Frank Eardley Endophytic Chassis Development View
MIC Plate Layout Frank Eardley Endophytic Chassis Development View
2-Part Gibson Assembly Frank Eardley Endophytic Chassis Development View
Amplification of gBlocks and Gradient PCR Heather Bottomley Naringenin Operon Biosynthesis View
Colony PCR of Transformed DH5α Cells Heather Bottomley Naringenin Operon Biosynthesis View
DpnI Digestion and Purification Heather Bottomley Naringenin Operon Biosynthesis View
Gel Extraction and Purification of the gBlocks Heather Bottomley Naringenin Operon Biosynthesis View
Gibson Assembly of gBlocks and pSB1C3 Backbone Heather Bottomley Naringenin Operon Biosynthesis View
Making Chemically Competent Cells Heather Bottomley Naringenin Operon Biosynthesis View
Miniprep of Transformed Colonies Heather Bottomley Naringenin Operon Biosynthesis View
Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR Heather Bottomley Naringenin Operon Biosynthesis View
Quantifying DNA Concentration Heather Bottomley Naringenin Operon Biosynthesis View
Resuspending gBlocks and Primers Heather Bottomley Naringenin Operon Biosynthesis View
SOC Media Preparation Heather Bottomley Naringenin Operon Biosynthesis View
Transformation of the Gibson assembly product into pSB1C3 Heather Bottomley Naringenin Operon Biosynthesis View




References & Attributions

Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley