Difference between revisions of "Team:Newcastle/Protocols"

 
(23 intermediate revisions by 5 users not shown)
Line 10: Line 10:
 
     <!-- home
 
     <!-- home
 
     ================================================== -->
 
     ================================================== -->
−
     <section id="home" class="s-home target-section" data-parallax="scroll" data-image-src="https://static.igem.org/mediawiki/2018/2/2d/T--Newcastle--ResultsHeader.jpeg">
+
     <section id="home" class="s-home target-section" data-parallax="scroll" data-image-src="https://static.igem.org/mediawiki/2018/4/4c/T--Newcastle--2345234.png">
  
 
         <div class="overlay"></div>
 
         <div class="overlay"></div>
Line 82: Line 82:
 
           <td>Chemotaxis</td>
 
           <td>Chemotaxis</td>
 
           <td><a href="https://static.igem.org/mediawiki/2018/8/89/T--Newcastle--Haemocytometer1.pdf" style="color:green">View</a></td>
 
           <td><a href="https://static.igem.org/mediawiki/2018/8/89/T--Newcastle--Haemocytometer1.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
<tr>
 +
        <td>Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide</td>
 +
        <td>Connor Trotter</td>
 +
        <td>Chemotaxis</td>
 +
        <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--ibidiprotocol.pdf" style="color:green">View<a/></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 94: Line 100:
 
         <td>Connor Trotter</td>
 
         <td>Connor Trotter</td>
 
         <td>Chemotaxis</td>
 
         <td>Chemotaxis</td>
−
         <td><a href="https://static.igem.org/mediawiki/2018/f/f3/T--Newcastle--AgarV2.pdf" style="color:green">View<a/></td>
+
         <td><a href="https://static.igem.org/mediawiki/2018/4/47/T--Newcastle--0.5ProtocolRedone.pdf" style="color:green">View<a/></td>
 +
        </tr>
 +
        <tr>
 +
        <td>0.50 % Agar Chemotaxis Assay</td>
 +
        <td>Connor Trotter</td>
 +
        <td>Chemotaxis</td>
 +
        <td><a href="https://static.igem.org/mediawiki/2018/2/2a/T--Newcastle--0.5%25ProtocolRedoneTrue.pdf" style="color:green">View<a/></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 102: Line 114:
 
           <td><a href="https://static.igem.org/mediawiki/2018/6/6a/T--Newcastle--GCIL.pdf" style="color:green">View</a></td>
 
           <td><a href="https://static.igem.org/mediawiki/2018/6/6a/T--Newcastle--GCIL.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 +
        <tr>
 +
        <tr>
 +
        <td>Quantitative Capillary Assay</td>
 +
          <td>Connor Trotter</td>
 +
          <td>Chemotaxis</td>
 +
          <td><a href="https://static.igem.org/mediawiki/2018/a/a1/T--Newcastle--CapillaryAssayChemo.pdf" style="color:green">View</a></td>
 +
        </tr>
 +
 
         <tr>
 
         <tr>
 
           <td>Naringenin Kill Curve in Liquid Culture</td>
 
           <td>Naringenin Kill Curve in Liquid Culture</td>
Line 124: Line 144:
 
           <td>Lewis Tomlinson</td>
 
           <td>Lewis Tomlinson</td>
 
           <td>Root Colonisation</td>
 
           <td>Root Colonisation</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/9/9e/T--Newcastle--Rack.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--RCK.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 130: Line 150:
 
           <td>Lewis Tomlinson</td>
 
           <td>Lewis Tomlinson</td>
 
           <td>Root Colonisation</td>
 
           <td>Root Colonisation</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/d/d7/T--Newcastle--ReIsolate.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/e/ef/T--Newcastle--RIso.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 142: Line 162:
 
           <td>Frank Eardley</td>
 
           <td>Frank Eardley</td>
 
           <td>Endophytic Chassis Development</td>
 
           <td>Endophytic Chassis Development</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/f/f9/T--Newcastle--MICCode.pdf" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/a/af/T--Newcastle--KillCurveCodes.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 166: Line 186:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/c/cb/T--Newcastle--GBlockAmp.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/8/87/T--Newcastle--GBAmp.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 172: Line 192:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/f/fb/T--Newcastle--PCRtransform.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/a/a9/T--Newcastle--PCTransform.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 178: Line 198:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/f/f3/T--Newcastle--DPN1digestion.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/b/b0/T--Newcastle--DPN1Diges.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 184: Line 204:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/d/d1/T--Newcastle--GBlockGelExt.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/c/cc/T--Newcastle--GBlockGel.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 190: Line 210:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/0/02/T--Newcastle--Gibson.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/1/15/T--Newcastle--Gib.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 196: Line 216:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/f/ff/T--Newcastle--CompCell.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/a/a6/T--Newcastle--CCell.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 202: Line 222:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/b/bf/T--Newcastle--Miniprep.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/0/02/T--Newcastle--MinPre.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 208: Line 228:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/c/c4/T--Newcastle--PCR.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/5/5f/T--Newcastle--PCReaction.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 214: Line 234:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/4/4f/T--Newcastle--QuantDNA.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/0/03/T--Newcastle--QuantifyingDNA.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 220: Line 240:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/3/32/T--Newcastle--GBlockFragments.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/a/a0/T--Newcastle--GBlockFragmnt.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 226: Line 246:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/9/99/T--Newcastle--SOC.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/4/4e/T--Newcastle--SOC1.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
Line 232: Line 252:
 
           <td>Heather Bottomley</td>
 
           <td>Heather Bottomley</td>
 
           <td>Naringenin Operon Biosynthesis</td>
 
           <td>Naringenin Operon Biosynthesis</td>
−
           <td><a href="https://static.igem.org/mediawiki/2018/2/2d/T--Newcastle--GBlockTransform.docx" style="color:green">View</a></td>
+
           <td><a href="https://static.igem.org/mediawiki/2018/1/15/T--Newcastle--GBTransform.pdf" style="color:green">View</a></td>
 
         </tr>
 
         </tr>
 
       </tbody>
 
       </tbody>
Line 281: Line 301:
  
 
     </div> <!-- end photoSwipe background -->
 
     </div> <!-- end photoSwipe background -->
 +
 +
<section id='team' class="s-services">
 +
 +
 +
        <div class="row section-header has-bottom-sep" data-aos="fade-up">
 +
                <div class="col-full">
 +
                            <br>
 +
<br>
 +
<br>
 +
<br>
 +
<h3 class="subhead"></h3>
 +
                <h1 class="display-2">References & Attributions</h1>
 +
            </div>
 +
 +
        </div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
<button class="collapsible">Click for References</button>
 +
<div class="content">
 +
              <div class="row about-desc" data-aos="fade-up">
 +
                <div class="col-full">
 +
 +
<p class="about-para"><font size="2"><strong>Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley
 +
</strong><font></p>
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</section>
 +
  
 
    
 
    
Line 291: Line 351:
 
     <script type="text/javascript" src="https://2018.igem.org/Template:Newcastle/JSmain?
 
     <script type="text/javascript" src="https://2018.igem.org/Template:Newcastle/JSmain?
 
     action=raw&ctype=text/javascript"></script>   
 
     action=raw&ctype=text/javascript"></script>   
 +
    <script>
 +
var coll = document.getElementsByClassName("collapsible");
 +
var i;
  
 +
for (i = 0; i < coll.length; i++) {
 +
  coll[i].addEventListener("click", function() {
 +
    this.classList.toggle("active");
 +
    var content = this.nextElementSibling;
 +
    if (content.style.maxHeight){
 +
      content.style.maxHeight = null;
 +
    } else {
 +
      content.style.maxHeight = content.scrollHeight + "px";
 +
    }
 +
  });
 +
}
 +
</script>
 
</body>
 
</body>
  
 
</html>
 
</html>
 
{{Newcastle/footer}}
 
{{Newcastle/footer}}

Latest revision as of 00:50, 18 October 2018

Alternative Roots/Protocols

Alternative Roots

Protocols

Overview

EXPERIMENTS

PROTOCOLS

Protocol Author Category PDF File
Glycerol Stock Preserves Connor Trotter General View
Bacterial Isolation from Freeze-Dried Pellet Connor Trotter General View
Cell Counting Connor Trotter Chemotaxis View
Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide Connor Trotter Chemotaxis View
Minimal A Salt Agar Chemotaxis Assay Connor Trotter Chemotaxis View
0.75 % Agar Chemotaxis Assay Connor Trotter Chemotaxis View
0.50 % Agar Chemotaxis Assay Connor Trotter Chemotaxis View
Growth Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Quantitative Capillary Assay Connor Trotter Chemotaxis View
Naringenin Kill Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Planting Seeds in Agar-Filled Microcentrifuge Tubes Lewis Tomlinson Root Colonisation View
Preparation and Staining of Microscopy Samples Lewis Tomlinson Root Colonisation View
Planting Seeds in Agar-Filled Pipette-Tip Racks Lewis Tomlinson Root Colonisation View
Re-isolating Endophytes Lewis Tomlinson Root Colonisation View
Electroporation of Pseudomonas sp. Frank Eardley Endophytic Chassis Development View
MIC Opentrons Code (2 Plates) Frank Eardley Endophytic Chassis Development View
MIC Protocol Frank Eardley Endophytic Chassis Development View
MIC Plate Layout Frank Eardley Endophytic Chassis Development View
2-Part Gibson Assembly Frank Eardley Endophytic Chassis Development View
Amplification of gBlocks and Gradient PCR Heather Bottomley Naringenin Operon Biosynthesis View
Colony PCR of Transformed DH5α Cells Heather Bottomley Naringenin Operon Biosynthesis View
DpnI Digestion and Purification Heather Bottomley Naringenin Operon Biosynthesis View
Gel Extraction and Purification of the gBlocks Heather Bottomley Naringenin Operon Biosynthesis View
Gibson Assembly of gBlocks and pSB1C3 Backbone Heather Bottomley Naringenin Operon Biosynthesis View
Making Chemically Competent Cells Heather Bottomley Naringenin Operon Biosynthesis View
Miniprep of Transformed Colonies Heather Bottomley Naringenin Operon Biosynthesis View
Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR Heather Bottomley Naringenin Operon Biosynthesis View
Quantifying DNA Concentration Heather Bottomley Naringenin Operon Biosynthesis View
Resuspending gBlocks and Primers Heather Bottomley Naringenin Operon Biosynthesis View
SOC Media Preparation Heather Bottomley Naringenin Operon Biosynthesis View
Transformation of the Gibson assembly product into pSB1C3 Heather Bottomley Naringenin Operon Biosynthesis View




References & Attributions

Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley