Difference between revisions of "Team:Newcastle/Protocols"
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<td>Chemotaxis</td> | <td>Chemotaxis</td> | ||
<td><a href="https://static.igem.org/mediawiki/2018/8/89/T--Newcastle--Haemocytometer1.pdf" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/8/89/T--Newcastle--Haemocytometer1.pdf" style="color:green">View</a></td> | ||
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| + | <td>Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide</td> | ||
| + | <td>Connor Trotter</td> | ||
| + | <td>Chemotaxis</td> | ||
| + | <td><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Newcastle--ibidiprotocol.pdf" style="color:green">View<a/></td> | ||
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<td>Connor Trotter</td> | <td>Connor Trotter</td> | ||
<td>Chemotaxis</td> | <td>Chemotaxis</td> | ||
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| − | <td>0. | + | <td>0.50 % Agar Chemotaxis Assay</td> |
<td>Connor Trotter</td> | <td>Connor Trotter</td> | ||
<td>Chemotaxis</td> | <td>Chemotaxis</td> | ||
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<td>Quantitative Capillary Assay</td> | <td>Quantitative Capillary Assay</td> | ||
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<td>Chemotaxis</td> | <td>Chemotaxis</td> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2018/ | + | <td><a href="https://static.igem.org/mediawiki/2018/a/a1/T--Newcastle--CapillaryAssayChemo.pdf" style="color:green">View</a></td> |
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<td>Naringenin Kill Curve in Liquid Culture</td> | <td>Naringenin Kill Curve in Liquid Culture</td> | ||
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<td>Frank Eardley</td> | <td>Frank Eardley</td> | ||
<td>Endophytic Chassis Development</td> | <td>Endophytic Chassis Development</td> | ||
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Latest revision as of 00:50, 18 October 2018
Alternative Roots
Protocols
EXPERIMENTS
PROTOCOLS
| Protocol | Author | Category | PDF File |
|---|---|---|---|
| Glycerol Stock Preserves | Connor Trotter | General | View |
| Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
| Cell Counting | Connor Trotter | Chemotaxis | View |
| Seeding Cells in ibidi µ-Slide III 3-in-1 Chemotaxis Microscopy Slide | Connor Trotter | Chemotaxis | View |
| Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| 0.75 % Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| 0.50 % Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| Growth Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Quantitative Capillary Assay | Connor Trotter | Chemotaxis | View |
| Naringenin Kill Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
| Planting Seeds in Agar-Filled Microcentrifuge Tubes | Lewis Tomlinson | Root Colonisation | View |
| Preparation and Staining of Microscopy Samples | Lewis Tomlinson | Root Colonisation | View |
| Planting Seeds in Agar-Filled Pipette-Tip Racks | Lewis Tomlinson | Root Colonisation | View |
| Re-isolating Endophytes | Lewis Tomlinson | Root Colonisation | View |
| Electroporation of Pseudomonas sp. | Frank Eardley | Endophytic Chassis Development | View |
| MIC Opentrons Code (2 Plates) | Frank Eardley | Endophytic Chassis Development | View |
| MIC Protocol | Frank Eardley | Endophytic Chassis Development | View |
| MIC Plate Layout | Frank Eardley | Endophytic Chassis Development | View |
| 2-Part Gibson Assembly | Frank Eardley | Endophytic Chassis Development | View |
| Amplification of gBlocks and Gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Colony PCR of Transformed DH5α Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| DpnI Digestion and Purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gel Extraction and Purification of the gBlocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gibson Assembly of gBlocks and pSB1C3 Backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Making Chemically Competent Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Miniprep of Transformed Colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Quantifying DNA Concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Resuspending gBlocks and Primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| SOC Media Preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Transformation of the Gibson assembly product into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |
References & Attributions
Attributions: Connor Trotter, Sadiya Quazi, Lewis Tomlinson, Frank Eardley, Heather Bottomley