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<body data-spy="scroll" data-target=".navbar-example"> | <body data-spy="scroll" data-target=".navbar-example"> | ||
<div class="container content"> | <div class="container content"> | ||
− | + | <div class="headstyle"> | |
+ | <h1 class="head">Model</h1> | ||
+ | </div> | ||
+ | <div class="righttitle"> | ||
+ | <h6 class="subtitle">Prediction of Metabolism</h6> | ||
+ | </div> | ||
<div class="navbar-example"> | <div class="navbar-example"> | ||
<div class="row"> | <div class="row"> | ||
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Therefore, we can save time to try and error on doing experiment. | Therefore, we can save time to try and error on doing experiment. | ||
After that, we analyze the rate of production and consumption. | After that, we analyze the rate of production and consumption. | ||
− | In this way, we can calculate the amount of | + | In this way, we can calculate the amount of CO<sub>2</sub> uptake into the |
− | <i>Escherichia coli </i> (<i>E. coli</i>) and | + | <i>Escherichia coli </i> ( <i>E. coli</i> ) and |
calculate how much CO<sub>2</sub> will be used in our system. | calculate how much CO<sub>2</sub> will be used in our system. | ||
− | In addition, we also want to realize how much carbon | + | In addition, we also want to realize how much carbon being fixed in our system. |
We have to understand the process in <i>E. coli</i> after uptaking | We have to understand the process in <i>E. coli</i> after uptaking | ||
CO<sub>2</sub>. | CO<sub>2</sub>. | ||
Since we integrated non-native carbon fixation pathway into <i>E. coli</i> to let | Since we integrated non-native carbon fixation pathway into <i>E. coli</i> to let | ||
<i>E. coli</i> utilize CO<sub>2</sub>, | <i>E. coli</i> utilize CO<sub>2</sub>, | ||
− | we | + | we simplify the CO<sub>2</sub> utilization pathway in engineered <i>E. coli</i> |
into two parts, | into two parts, | ||
<a class="link" href="#CO2_uptake">CO<sub>2</sub> uptake</a> and <a class="link" href="#CO2_metabolism">CO<sub>2</sub> metabolism</a>. | <a class="link" href="#CO2_uptake">CO<sub>2</sub> uptake</a> and <a class="link" href="#CO2_metabolism">CO<sub>2</sub> metabolism</a>. | ||
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<div class="col-12" id="resultimg"> | <div class="col-12" id="resultimg"> | ||
<img class="twoimg" src="https://static.igem.org/mediawiki/2018/7/75/T--NCKU_Tainan--model_result1.png"> | <img class="twoimg" src="https://static.igem.org/mediawiki/2018/7/75/T--NCKU_Tainan--model_result1.png"> | ||
− | <p class="pcenter">Fig. | + | <p class="pcenter">Fig 1. CO<sub>2</sub> uptake amount change with time in engineered <i>E. coli</i> without CA gene.</p> |
<img class="twoimg" src="https://static.igem.org/mediawiki/2018/9/9f/T--NCKU_Tainan--model_result2.png"> | <img class="twoimg" src="https://static.igem.org/mediawiki/2018/9/9f/T--NCKU_Tainan--model_result2.png"> | ||
− | <p class="pcenter">Fig. | + | <p class="pcenter">Fig 2. CO<sub>2</sub> uptake amount change with time in engineered <i>E. coli</i> with CA gene.</p> |
</div> | </div> | ||
</div> | </div> | ||
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<img class="col-md-6" id="easypathway" src="https://static.igem.org/mediawiki/2018/f/f8/T--NCKU_Tainan--model_easypathway.png"> | <img class="col-md-6" id="easypathway" src="https://static.igem.org/mediawiki/2018/f/f8/T--NCKU_Tainan--model_easypathway.png"> | ||
<img class="col-md-6 contentimg" src="https://static.igem.org/mediawiki/2018/3/3e/T--NCKU_Tainan--model_pathway.png"> | <img class="col-md-6 contentimg" src="https://static.igem.org/mediawiki/2018/3/3e/T--NCKU_Tainan--model_pathway.png"> | ||
− | <p class="pcenter col-12">Fig. | + | <p class="pcenter col-12">Fig 3. Metabolic pathway of CO<sub>2</sub>-utilization <i>E. coli</i>. </p> |
− | <p class="pcontent">The introduced CO<sub>2</sub>-utilization bypass pathway composed of PRK and Rubisco is drawn in green line and noted by ” A” reaction and the double line was the pathway that composed by genetically modified, while the central carbon metabolic pathway including PP pathway and TCA cycle is drawn in blue line and yellow line and noted by “B” reaction and “C” reaction, respectively. | + | <p class="pcontent col-12">The introduced CO<sub>2</sub>-utilization bypass pathway composed of PRK and Rubisco is drawn in green line and noted by ” A” reaction and the double line was the pathway that composed by genetically modified, while the central carbon metabolic pathway including PP pathway and TCA cycle is drawn in blue line and yellow line and noted by “B” reaction and “C” reaction, respectively. |
See more detail in <a class="link" href="https://2018.igem.org/Team:NCKU_Tainan/Kinetic_Law">Kinetic law</a>. | See more detail in <a class="link" href="https://2018.igem.org/Team:NCKU_Tainan/Kinetic_Law">Kinetic law</a>. | ||
</p> | </p> | ||
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<p class="pcontent">Two main sources of CO<sub>2</sub> metabolism in engineered <i>E. coli</i> are xylose and CO<sub>2</sub>. | <p class="pcontent">Two main sources of CO<sub>2</sub> metabolism in engineered <i>E. coli</i> are xylose and CO<sub>2</sub>. | ||
CO<sub>2</sub> utilization rate varies under different condition. | CO<sub>2</sub> utilization rate varies under different condition. | ||
− | We use 5% CO<sub>2</sub> (about 2.6 mM) and | + | We use 5% CO<sub>2</sub> (about 2.6 mM) and 4 (g/l) xylose (about 26 mM) in experiment as an optimization CO<sub>2</sub> utilization rate. |
Constant CO<sub>2</sub> condition and limited CO<sub>2</sub> condition also effects metabolism performance. | Constant CO<sub>2</sub> condition and limited CO<sub>2</sub> condition also effects metabolism performance. | ||
In other words, open system and close system showed different results. | In other words, open system and close system showed different results. | ||
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<h3>References</h3> | <h3>References</h3> | ||
<ol> | <ol> | ||
− | <li class="smallp"> | + | <li class="smallp">E. G. Jacqueline, P. L. Christopher, R. A. Maciek, Comprehensive analysis of glucose and xylose metabolism in <i>Escherichia coli</i> under aerobic and anaerobic conditions by <sup>13</sup>C metabolic flux analysis. Metab Eng. 2017 Jan; 39: 9–18.</li> |
− | <li class="smallp"> | + | <li class="smallp">U. Sauer, J. E. Bernhard, The PEP—pyruvate—oxaloacetate node as the switch point for carbon flux distribution in bacteria. FEMS Microbiology Reviews, Volume 29, Issue 4, 1 September 2005, Pages 765–794.</li> |
− | <li class="smallp"> | + | <li class="smallp">G. Fuyu, L. Guoxia, Z. Xiaoyun, Z. Jie, C. Zhen, L. Yin . Quantitative analysis of an engineered CO<sub>2</sub>-fixing <i>Escherichia coli</i>reveals great potential of heterotrophic CO<sub>2</sub> fixation. Gong et al. Biotechnology for Biofuels, 2015, 8:86.</li> |
− | <li class="smallp">Y. Pocker, | + | <li class="smallp">Y. Pocker, S. Y. Ng. Joan, Plant carbonic anhydrase. Properties and carbon dioxide hydration kinetics. Biochemistry, 1973, 12 (25), pp 5127–5134.</li> |
</ol> | </ol> | ||
<br class="pcontent"> | <br class="pcontent"> | ||
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} | } | ||
} else { | } else { | ||
− | if ($(this).scrollTop() >= | + | if ($(this).scrollTop() >= 500) { |
var position = $("#sidelist").position(); | var position = $("#sidelist").position(); | ||
if(position == undefined){} | if(position == undefined){} |
Latest revision as of 01:25, 18 October 2018