Difference between revisions of "Team:Newcastle/Collaborations"

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                             <h3 class="subhead">COLLABORATIONS</h3>
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                 <h1 class="display-2" style="margin-bottom:0px" >Exeter University</h1>
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                     <p><font size="3"><br><br>The United Nations estimates global population has increased by 1 billion over 12 years and will near 9.8 billion by 2050 [4]. This increase has meant social demand has also grown at an unparalleled rate. However, a more pressing issues is that of food security. Food security is defined as one’s ability to have adequate access to sufficient food. Said food must be safe and nutritious as for an individual to maintain a healthy and active lifestyle. </font></p>
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                     <p><font size="3"><br><br>Perchlorate salts are found in the Martian regolith covering the surface of Mars at a concentration of up to 1 %. Perchlorate is a highly oxidising compound used in rocket fuels and munitions. It is dangerous and toxic to humans. Current disposal methods are via expensive bioreactors, or contained explosions. So, if humans are to colonise Mars, we will have to remove the perchlorates from the soil in order to have a chance of developing agriculture for our survival. The University of <a href="https://2018.igem.org/Team:Exeter/Collaborations" class="black">Exeter</a> 2018 iGEM Team are developing a bioreactor that will remove perchlorate salts from soil, and dispose of waste perchlorate in a cheap, safe manner, by using genetically engineered bacteria with (per)chlorate and chlorite reducing enzymes.</font></p>
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<p><font size="3">To assist their project, we have recorded the affects of perchlorate on the growth of our free-living nitrogen fixing, soil bacteria. This has helped show the importance of their project in aiding the development of agricultural methods on Mars, as well as disposing of perchlorate in a safe, contained way so as not to harm the environment and affect soil ecosystems. The results of our experiments are displayed in Figures 1 to 3.</font></a><br><br>
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<p><font size="2"><center>Figure 1. <i>Azorhizobium caulidolens</i> grown in LB broth containing NaCIO<sub>4</sub> at varying concentrations. Cells were grown in 96-well plate format in 200 µl volumes at 30 °C over 24 hours. (n=3 replicates, error bars are standard error of the mean).</center></font></p>
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<p><font size="2"><center>Figure 2. <i>Azospirillum brasilense</i> grown in LB broth containing NaCIO<sub>4</sub> at varying concentrations. Cells were grown in 96-well plate format in 200 µl volumes at 30 °C over 24 hours. (n=3 replicates, error bars are standard error of the mean).</center></font></p>
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<p><font size="2"><center>Figure 3. <i>Herbosporillum seropedicae</i> grown in LB broth containing NaCIO<sub>4</sub> at varying concentrations. Cells were grown in 96-well plate format in 200 µl volumes at 30 °C over 24 hours. (n=3 replicates, error bars are standard error of the mean).</center></font></p>
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                 <h1 class="display-2">Sorbonne Université Paris</h1>
 
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                    <p><font size="3"><br><br>One of our team members has a friend at <a href="https://2018.igem.org/Team:Sorbonne_U_Paris/Collaborations" class="black">Sorbonne</a>, who kindly put us in contact with their iGEM team. As they knew we have architecture students in the team they asked us to produce a visual for their human practices.
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They sent us over an initial sketch, once this was received we used our imaginations and attempted to visualise this part of their project for them. The image shows a semi-permeable membrane which would contain their genetically modified bacteria, anchored to the sea bed - but not so deep that they wouldn’t receive any light. This would prevent their bacteria from being released into the environment. <br>
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                    <p><font size="3">The United Nations estimates global population has increased by 1 billion over 12 years and will near 9.8 billion by 2050 [4]. This increase has meant social demand has also grown at an unparalleled rate. However, a more pressing issues is that of food security.  Food security is defined as one’s ability to have adequate access to sufficient food. Said food must be safe and nutritious as for an individual to maintain a healthy and active lifestyle. </font></p>
 
  
 
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                     <p><font size="3">The United Nations estimates global population has increased by 1 billion over 12 years and will near 9.8 billion by 2050 [4]. This increase has meant social demand has also grown at an unparalleled rate. However, a more pressing issues is that of food security.  Food security is defined as one’s ability to have adequate access to sufficient food. Said food must be safe and nutritious as for an individual to maintain a healthy and active lifestyle. </font></p>
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                     <p><font size="3"><br><br>To aid <a href="https://2018.igem.org/Team:Warwick/Collaborations" class="black">Warwick</a> in their project we collected a series of water samples from bodies of water in areas surrounding Newcastle.
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                <h1 class="display-2">References & Attributions</h1>
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<p class="about-para"><font size="2"><strong>Attributions: Frank Eardley, Sadiya Quazi, Chris Carty, Lewis Tomlinson and Will Tankard
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Latest revision as of 02:15, 18 October 2018

Alternative Roots/Protocols

Collaborations

Exeter Sorbonne Warwick

University of Exeter



Perchlorate salts are found in the Martian regolith covering the surface of Mars at a concentration of up to 1 %. Perchlorate is a highly oxidising compound used in rocket fuels and munitions. It is dangerous and toxic to humans. Current disposal methods are via expensive bioreactors, or contained explosions. So, if humans are to colonise Mars, we will have to remove the perchlorates from the soil in order to have a chance of developing agriculture for our survival. The University of Exeter 2018 iGEM Team are developing a bioreactor that will remove perchlorate salts from soil, and dispose of waste perchlorate in a cheap, safe manner, by using genetically engineered bacteria with (per)chlorate and chlorite reducing enzymes.

To assist their project, we have recorded the affects of perchlorate on the growth of our free-living nitrogen fixing, soil bacteria. This has helped show the importance of their project in aiding the development of agricultural methods on Mars, as well as disposing of perchlorate in a safe, contained way so as not to harm the environment and affect soil ecosystems. The results of our experiments are displayed in Figures 1 to 3.

Figure 1. Azorhizobium caulidolens grown in LB broth containing NaCIO4 at varying concentrations. Cells were grown in 96-well plate format in 200 µl volumes at 30 °C over 24 hours. (n=3 replicates, error bars are standard error of the mean).

Figure 2. Azospirillum brasilense grown in LB broth containing NaCIO4 at varying concentrations. Cells were grown in 96-well plate format in 200 µl volumes at 30 °C over 24 hours. (n=3 replicates, error bars are standard error of the mean).

Figure 3. Herbosporillum seropedicae grown in LB broth containing NaCIO4 at varying concentrations. Cells were grown in 96-well plate format in 200 µl volumes at 30 °C over 24 hours. (n=3 replicates, error bars are standard error of the mean).

Sorbonne Université Paris



One of our team members has a friend at Sorbonne, who kindly put us in contact with their iGEM team. As they knew we have architecture students in the team they asked us to produce a visual for their human practices. They sent us over an initial sketch, once this was received we used our imaginations and attempted to visualise this part of their project for them. The image shows a semi-permeable membrane which would contain their genetically modified bacteria, anchored to the sea bed - but not so deep that they wouldn’t receive any light. This would prevent their bacteria from being released into the environment.

University of Warwick




To aid Warwick in their project we collected a series of water samples from bodies of water in areas surrounding Newcastle.







References & Attributions

Attributions: Frank Eardley, Sadiya Quazi, Chris Carty, Lewis Tomlinson and Will Tankard