Difference between revisions of "Team:Lethbridge"

Line 25: Line 25:
 
<div class="imageText-Wrapper">
 
<div class="imageText-Wrapper">
 
<div class="imageText-Image">
 
<div class="imageText-Image">
<center><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Lethbridge--vincentFace.png" width=40%></center>
+
<center><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Lethbridge--vincentFace.png" width=55%></center>
 
</div>
 
</div>
 
<div class="imageText-Text">
 
<div class="imageText-Text">
Line 60: Line 60:
 
<div style="clear: both"></div><br>
 
<div style="clear: both"></div><br>
  
<h1>Cell-free Synthesis Components</h1>
+
<h1>Cell-free Synthesis</h1>
 
<div class="oneText-Wrapper">
 
<div class="oneText-Wrapper">
 
<div class="oneText-Text">
 
<div class="oneText-Text">
<p class="f12"><b>Fifteen</b> parts were improved from our <a href="https://2017.igem.org/Team:Lethbridge" target="_blank">2017 N<i>ex</i>t <i>Vivo</i></a> project. Our goal with these parts was to continue standardizing, improving, and simplifying our protocols and we are now beginning design of cell-free PNC synthesis.
+
<p class="f12"><b>Fifteen</b> parts were improved from our <a href="https://2017.igem.org/Team:Lethbridge" target="_blank">2017 N<i>ex</i>t <i>Vivo</i></a> project. Our goal was to continue standardizing, improving, and simplifying our protocols and we are now beginning design of cell-free PNC synthesis with these components.
 
</p>
 
</p>
 
</div>
 
</div>

Revision as of 02:29, 18 October 2018



Main Page Banner



Protein Nanocompartments

VINCEnT is a standardized toolkit for simple design and assembly of custom protein nanocompartments (PNCs) for delivery of small molecules, nucleic acids, or proteins to specific cell targets. PNCs have user-specified surface proteins, encapsulation proteins, and cargo-loading approaches with wide-ranged applicability including targeted antibiotic delivery, rapid vaccine development, gene therapy, materials synthesis, improved cell culture transfection, specific biological control agents, and more.


Software

To enhance the accessibility of this toolkit, a software tool is in development that enables users to design a novel PNC to their own specifications based on suggested parts for their intended application. Standardized protocols will accompany the pre-designed toolkit parts and, together with the simplified design, less experienced users will be able to safely conduct complex experiments in a basic laboratory environment.


Risk Assessment Rubric

A risk assessment rubric was developed for our toolkit with advice from PHAC (Public Health Agency of Canada) and members of ELBI (Emerging Leaders in Biosecurity Initiative).


Cell-free Synthesis

Fifteen parts were improved from our 2017 Next Vivo project. Our goal was to continue standardizing, improving, and simplifying our protocols and we are now beginning design of cell-free PNC synthesis with these components.