Difference between revisions of "Team:Lethbridge"

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<center><h1>Protein Nanocompartments</h1></center>
  
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<center><img src="https://static.igem.org/mediawiki/2018/e/e9/T--Lethbridge--vincentFace.png" width=55%></center>
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<p class="f12">VINCEnT is a standardized toolkit for simple design and assembly of custom protein nanocompartments (PNCs) for delivery of small molecules, nucleic acids, or proteins to specific cell targets. PNCs have user-specified surface proteins, encapsulation proteins, and cargo-loading approaches with wide-ranged applicability including targeted antibiotic delivery, rapid vaccine development, gene therapy, materials synthesis, improved cell culture transfection, specific biological control agents, and more. </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
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<h1>Software</h1>
  
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<p class="f12">To enhance the accessibility of this toolkit, we designed a basic software tool that enables users to design a novel PNC to their own specifications based on suggested parts for their intended application. Standardized protocols accompany the pre-designed toolkit parts and, together with the simplified design, less experienced users are able to safely conduct complex experiments in a basic laboratory environment.</p>
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<center><img src="https://static.igem.org/mediawiki/2018/6/60/T--Lethbridge--computer_vincent.png" width: 60%></center>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h1>Risk Assessment Rubric</h1>
  
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<a href="https://2018.igem.org/wiki/index.php?title=Team:Lethbridge&action=edit">  
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EDIT PAGE
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<p class="f12">A risk assessment rubric was developed for our toolkit with advice from PHAC (Public Health Agency of Canada) and members of ELBI (Emerging Leaders in Biosecurity Initiative).</p>
 
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<h1>Cell-free Synthesis</h1>
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<p class="f12"><b>Fifteen</b> parts were improved from our <a href="https://2017.igem.org/Team:Lethbridge" target="_blank">2017 N<i>ex</i>t <i>Vivo</i></a> project. Our goal was to continue standardizing, improving, and simplifying our protocols and we are now beginning design of cell-free PNC synthesis with these components.
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<img src="https://static.igem.org/mediawiki/2018/6/6e/T--Lethbridge--next_vivo.png">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 02:36, 18 October 2018



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Protein Nanocompartments

VINCEnT is a standardized toolkit for simple design and assembly of custom protein nanocompartments (PNCs) for delivery of small molecules, nucleic acids, or proteins to specific cell targets. PNCs have user-specified surface proteins, encapsulation proteins, and cargo-loading approaches with wide-ranged applicability including targeted antibiotic delivery, rapid vaccine development, gene therapy, materials synthesis, improved cell culture transfection, specific biological control agents, and more.


Software

To enhance the accessibility of this toolkit, we designed a basic software tool that enables users to design a novel PNC to their own specifications based on suggested parts for their intended application. Standardized protocols accompany the pre-designed toolkit parts and, together with the simplified design, less experienced users are able to safely conduct complex experiments in a basic laboratory environment.


Risk Assessment Rubric

A risk assessment rubric was developed for our toolkit with advice from PHAC (Public Health Agency of Canada) and members of ELBI (Emerging Leaders in Biosecurity Initiative).


Cell-free Synthesis

Fifteen parts were improved from our 2017 Next Vivo project. Our goal was to continue standardizing, improving, and simplifying our protocols and we are now beginning design of cell-free PNC synthesis with these components.