Difference between revisions of "Team:NCTU Formosa/Composite Part"

We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

Bacteriocins

Bovicin HJ50-Intein-CBD(BBa_K2599009)

Enterocin B-Intein-CBD(BBa_K2599011)

Enterocin 96-Intein-CBD(BBa_K2599012)

Lacticin Z-Intein-CBD(BBa_K2599013)

Leucocyclicin Q-Intein-CBD(BBa_K2599014)

Durancin TW-49M-Intein-CBD(BBa_K2599015)

Curcumin Bio-sensor

GS Linker-αS1-casein(BBa_K2599017)

Improvement

Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)

Template

@@ Line 32: / Line 32: @@
        width: 100%;
        font-family: Levenim MT;
-      background: #f0feff;
      }
@@ Line 38: / Line 37: @@
        width: 100%;
        overflow: hidden;
+      background-color: white;
+      padding-bottom: 150px;
      }
@@ Line 53: / Line 54: @@
      .text{
-       font-size: 4vmin;
+       font-size: 3vmin;
-       width: 50%;
+       width: 70%;
        position: relative;
-       margin-left: 25%;
+       margin-left: 15%;
        margin-top: 40px;
        margin-bottom: 40px;
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        transition-duration: 0.4s;
        position: relative;
-       left: 25%;
+       left: 15%;
        margin: 30px;
      }
@@ Line 94: / Line 95: @@
      .bac {
+      width: 40%;
+      margin: 50px;
+      margin-left: 30%;
+      margin-bottom: 70px;
+    }
+    .curcumin {
        width: 40%;
        margin: 50px;
@@ Line 112: / Line 120: @@
      }
-    .basic_parts {
-      display: none;
-    }
      .part_button {
@@ Line 171: / Line 176: @@
    <div class="wrapper">
      <div class="banner">
-       <img class="cover" src="https://static.igem.org/mediawiki/2018/e/ed/T--NCTU_Formosa--Parts.png">
+       <img class="cover" src="https://static.igem.org/mediawiki/2018/6/63/T--NCTU_Formosa--Parts2.0.png">
      </div>
      <div class="sec1">
        <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title">
-      <img src="https://static.igem.org/mediawiki/2018/5/5d/T--NCTU_Formosa--Parts_dec3.png" class="dec_1">
-      <div class="title_1"><p>Overview</p></div>
        <div class="text">
          <p>
-           This year we decided to create nine basic parts. These parts contain the single sequences of each antimicrobial peptide. Our goal is to assemble these basic parts with intein and chitin binding domain (CBD) sequences, which are better to purify. We listed all the basic parts we constructed below.
+           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
+          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>.
          </p>
        </div>
@@ Line 186: / Line 190: @@
 <!--------------------------------------------------------------------->
        <div class="basic_parts">
-         <div class="title_1"><p>Antimicrobial Peptide</p></div>
+         <div class="title_1"><p>Bacteriocins</p></div>
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599000" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin-Intein-CBD(BBa_K2599008)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
-        <img src="https://static.igem.org/mediawiki/2018/b/bf/T--NCTU_Formosa--Sub_2.png" class="bac">
-        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599001" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599002" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin A-Intein-CBD(BBa_K2599010)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
-        <img src="https://static.igem.org/mediawiki/2018/4/45/T--NCTU_Formosa--Ent_A_2.png" class="bac">
-        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599003" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599004" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96-Intein-CBD(BBa_K2599012)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599012" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96-Intein-CBD(BBa_K2599012)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/d/da/T--NCTU_Formosa--Ent_96_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599005" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z-Intein-CBD(BBa_K2599013)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599013" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z-Intein-CBD(BBa_K2599013)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/8/8b/T--NCTU_Formosa--Lac_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599006" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q-Intein-CBD(BBa_K2599014)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599014" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q-Intein-CBD(BBa_K2599014)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/1/13/T--NCTU_Formosa--Leu_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599007" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
-      </div>
-      <img src="https://static.igem.org/mediawiki/2018/9/99/T--NCTU_Formosa--Parts_button.png" class="part_button" id="pb" onmouseup="mouseUp()">
+        <div class="title_1"><p>Curcumin Bio-sensor</p></div>
-      <img src="https://static.igem.org/mediawiki/2018/6/61/T--NCTU_Formosa--Parts_dec4.png" class="dec_2">
+        <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a>
+        <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;">
+        <div class="title_1"><p>Improvement</p></div>
+        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a>
+        <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;">
+      </div>
      </div>
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 {{NCTU_Formosa/Footer}}
+{{NCTU_Formosa/Top_button}}