Difference between revisions of "Team:NCTU Formosa/Composite Part"

 
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       font-family: Levenim MT;
 
       font-family: Levenim MT;
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     }
 
     }
  
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     .text{
 
     .text{
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       position: relative;
 
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     }
 
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     .bac {
 
     .bac {
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      margin-bottom: 70px;
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    }
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    .curcumin {
 
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     }
 
     }
  
    .basic_parts {
 
      display: none;
 
    }
 
  
 
     .part_button {
 
     .part_button {
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   <div class="wrapper">
 
   <div class="wrapper">
 
     <div class="banner">
 
     <div class="banner">
       <img class="cover" src="https://static.igem.org/mediawiki/2018/e/ed/T--NCTU_Formosa--Parts.png">
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       <img class="cover" src="https://static.igem.org/mediawiki/2018/6/63/T--NCTU_Formosa--Parts2.0.png">
 
     </div>
 
     </div>
 
     <div class="sec1">
 
     <div class="sec1">
 
       <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title">
 
       <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title">
      <img src="https://static.igem.org/mediawiki/2018/5/5d/T--NCTU_Formosa--Parts_dec3.png" class="dec_1">
 
      <div class="title_1"><p>Overview</p></div>
 
 
       <div class="text">
 
       <div class="text">
 
         <p>
 
         <p>
           This year we decided to create nine basic parts. These parts contain the single sequences of each antimicrobial peptide. Our goal is to assemble these basic parts with intein and chitin binding domain (CBD) sequences, which are better to purify. We listed all the basic parts we constructed below.
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           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>.
 
         </p>
 
         </p>
 
       </div>
 
       </div>
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<!--------------------------------------------------------------------->
 
<!--------------------------------------------------------------------->
 
       <div class="basic_parts">
 
       <div class="basic_parts">
         <div class="title_1"><p>Antimicrobial Peptide</p></div>
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         <div class="title_1"><p>Bacteriocins</p></div>
        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599008" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin-Intein-CBD(BBa_K2599008)</p></a>
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        <img src="https://static.igem.org/mediawiki/2018/b/bf/T--NCTU_Formosa--Sub_2.png" class="bac">
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
 
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
 
         <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac">
 
         <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac">
        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599010" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin A-Intein-CBD(BBa_K2599010)</p></a>
 
        <img src="https://static.igem.org/mediawiki/2018/4/45/T--NCTU_Formosa--Ent_A_2.png" class="bac">
 
 
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
 
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
 
         <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac">
 
         <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac">
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
 
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
 
         <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
 
         <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
      </div>
 
  
      <img src="https://static.igem.org/mediawiki/2018/9/99/T--NCTU_Formosa--Parts_button.png" class="part_button" id="pb" onmouseup="mouseUp()">
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        <div class="title_1"><p>Curcumin Bio-sensor</p></div>
      <img src="https://static.igem.org/mediawiki/2018/6/61/T--NCTU_Formosa--Parts_dec4.png" class="dec_2">
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        <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a>
 +
        <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;">
 +
 
 +
        <div class="title_1"><p>Improvement</p></div>
 +
        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a>
 +
        <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;">
 +
      </div>
  
 
     </div>
 
     </div>
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{{NCTU_Formosa/Footer}}
 
{{NCTU_Formosa/Footer}}
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{{NCTU_Formosa/Top_button}}

Latest revision as of 02:58, 18 October 2018

Navigation Bar Composite Parts

     We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

     The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

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