Difference between revisions of "Team:NCTU Formosa/Composite Part"

We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

Bacteriocins

Bovicin HJ50-Intein-CBD(BBa_K2599009)

Enterocin B-Intein-CBD(BBa_K2599011)

Enterocin 96-Intein-CBD(BBa_K2599012)

Lacticin Z-Intein-CBD(BBa_K2599013)

Leucocyclicin Q-Intein-CBD(BBa_K2599014)

Durancin TW-49M-Intein-CBD(BBa_K2599015)

Curcumin Bio-sensor

GS Linker-αS1-casein(BBa_K2599017)

Improvement

Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)

Template

@@ Line 4: / Line 4: @@
 <head>
    <meta charset="UTF-8">
-   <title>Basic Parts</title>
+   <title>Composite Parts</title>
    <script src="jQueryAssets/jquery-1.11.1.min.js"></script>
@@ Line 38: / Line 38: @@
        overflow: hidden;
        background-color: white;
+      padding-bottom: 150px;
      }
@@ Line 53: / Line 54: @@
      .text{
-       font-size: 4vmin;
+       font-size: 3vmin;
-       width: 50%;
+       width: 70%;
        position: relative;
-       margin-left: 25%;
+       margin-left: 15%;
        margin-top: 40px;
        margin-bottom: 40px;
@@ Line 74: / Line 75: @@
      .basic_title {
-       width: 30%;
+       width: 35%;
-       margin-left: 35%;
+       margin-left: 32.5%;
        margin-top: 100px;
      }
@@ Line 85: / Line 86: @@
        transition-duration: 0.4s;
        position: relative;
-       left: 25%;
+       left: 15%;
        margin: 30px;
      }
@@ Line 94: / Line 95: @@
      .bac {
-       width: 20%;
+       width: 40%;
        margin: 50px;
-       margin-left: 40%;
+       margin-left: 30%;
+      margin-bottom: 70px;
+    }
+    .curcumin {
+      width: 40%;
+      margin: 50px;
+      margin-left: 30%;
        margin-bottom: 70px;
      }
@@ Line 112: / Line 120: @@
      }
-    .basic_parts {
-      display: none;
-      -webkit-transition-timing-function: ease-in;
-      -o-transition-timing-function: ease-in;
-      transition-timing-function: ease-in;
-    }
      .part_button {
@@ Line 177: / Line 179: @@
      </div>
      <div class="sec1">
-       <img src="https://static.igem.org/mediawiki/2018/a/a5/T--NCTU_Formosa--Basic_parts.png" class="basic_title">
+       <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title">
-      <img src="https://static.igem.org/mediawiki/2018/9/95/T--NCTU_Formosa--Parts_dec1.png" class="dec_1">
-      <div class="title_1"><p>Overview</p></div>
        <div class="text">
          <p>
-           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This year we decided to create eight basic parts. These parts contain the single sequences of each antimicrobial peptide. They all show the inhibition activity against Bacillus subtilis, which is one of the example of our system. We listed all the basic parts we constructed below.
+           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
+          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>.
          </p>
        </div>
@@ Line 190: / Line 191: @@
        <div class="basic_parts">
          <div class="title_1"><p>Bacteriocins</p></div>
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599000" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin(BBa_K2599000)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
-        <img src="https://static.igem.org/mediawiki/2018/2/2c/T--NCTU_Formosa--Sub.png" class="bac">
+         <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac">
-        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599001" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50(BBa_K2599001)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
-         <img src="https://static.igem.org/mediawiki/2018/f/f2/T--NCTU_Formosa--Bov.png" class="bac">
+         <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599002" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin A(BBa_K2599002)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599012" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96-Intein-CBD(BBa_K2599012)</p></a>
-        <img src="https://static.igem.org/mediawiki/2018/e/e5/T--NCTU_Formosa--Ent_A.png" class="bac">
+         <img src="https://static.igem.org/mediawiki/2018/d/da/T--NCTU_Formosa--Ent_96_2.png" class="bac">
-        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599003" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B(BBa_K2599003)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599013" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z-Intein-CBD(BBa_K2599013)</p></a>
-         <img src="https://static.igem.org/mediawiki/2018/4/4a/T--NCTU_Formosa--Ent_B.png" class="bac">
+         <img src="https://static.igem.org/mediawiki/2018/8/8b/T--NCTU_Formosa--Lac_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599004" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96(BBa_K2599004)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599014" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q-Intein-CBD(BBa_K2599014)</p></a>
-         <img src="https://static.igem.org/mediawiki/2018/3/35/T--NCTU_Formosa--Ent_96.png" class="bac">
+         <img src="https://static.igem.org/mediawiki/2018/1/13/T--NCTU_Formosa--Leu_2.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599005" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z(BBa_K2599005)</p></a>
+         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
-         <img src="https://static.igem.org/mediawiki/2018/d/de/T--NCTU_Formosa--Lac.png" class="bac">
+         <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599006" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q(BBa_K2599006)</p></a>
-         <img src="https://static.igem.org/mediawiki/2018/d/d5/T--NCTU_Formosa--Leu.png" class="bac">
-         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599007" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M(BBa_K2599007)</p></a>
-         <img src="https://static.igem.org/mediawiki/2018/a/a3/T--NCTU_Formosa--Dur.png" class="bac" style="margin-bottom: 50px;" >
-      </div>
-      <img src="https://static.igem.org/mediawiki/2018/9/99/T--NCTU_Formosa--Parts_button.png" class="part_button" id="pb" onmouseup="mouseUp()">
+        <div class="title_1"><p>Curcumin Bio-sensor</p></div>
-      <img src="https://static.igem.org/mediawiki/2018/a/ad/T--NCTU_Formosa--Parts_dec2.png" class="dec_2">
+        <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a>
+        <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;">
+        <div class="title_1"><p>Improvement</p></div>
+        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a>
+        <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;">
+      </div>
      </div>
@@ Line 226: / Line 228: @@
 {{NCTU_Formosa/Footer}}
+{{NCTU_Formosa/Top_button}}