We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
Difference between revisions of "Team:NCTU Formosa/Composite Part"
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<head> | <head> | ||
<meta charset="UTF-8"> | <meta charset="UTF-8"> | ||
| − | <title> | + | <title>Composite Parts</title> |
<script src="jQueryAssets/jquery-1.11.1.min.js"></script> | <script src="jQueryAssets/jquery-1.11.1.min.js"></script> | ||
| Line 38: | Line 38: | ||
overflow: hidden; | overflow: hidden; | ||
background-color: white; | background-color: white; | ||
| + | padding-bottom: 150px; | ||
} | } | ||
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.text{ | .text{ | ||
| − | font-size: | + | font-size: 3vmin; |
| − | width: | + | width: 70%; |
position: relative; | position: relative; | ||
| − | margin-left: | + | margin-left: 15%; |
margin-top: 40px; | margin-top: 40px; | ||
margin-bottom: 40px; | margin-bottom: 40px; | ||
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.basic_title { | .basic_title { | ||
| − | width: | + | width: 35%; |
| − | margin-left: | + | margin-left: 32.5%; |
margin-top: 100px; | margin-top: 100px; | ||
} | } | ||
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transition-duration: 0.4s; | transition-duration: 0.4s; | ||
position: relative; | position: relative; | ||
| − | left: | + | left: 15%; |
margin: 30px; | margin: 30px; | ||
} | } | ||
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.bac { | .bac { | ||
| − | width: | + | width: 40%; |
margin: 50px; | margin: 50px; | ||
| − | margin-left: 40%; | + | margin-left: 30%; |
| + | margin-bottom: 70px; | ||
| + | } | ||
| + | |||
| + | .curcumin { | ||
| + | width: 40%; | ||
| + | margin: 50px; | ||
| + | margin-left: 30%; | ||
margin-bottom: 70px; | margin-bottom: 70px; | ||
} | } | ||
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} | } | ||
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.part_button { | .part_button { | ||
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</div> | </div> | ||
<div class="sec1"> | <div class="sec1"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title"> |
| − | + | ||
| − | + | ||
<div class="text"> | <div class="text"> | ||
<p> | <p> | ||
| − | | + | We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br> |
| + | The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>. | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 190: | Line 191: | ||
<div class="basic_parts"> | <div class="basic_parts"> | ||
<div class="title_1"><p>Bacteriocins</p></div> | <div class="title_1"><p>Bacteriocins</p></div> | ||
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a> |
| − | + | <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac"> | |
| − | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a> | |
| − | <img src="https://static.igem.org/mediawiki/2018/f/ | + | <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac"> |
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599012" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96-Intein-CBD(BBa_K2599012)</p></a> |
| − | + | <img src="https://static.igem.org/mediawiki/2018/d/da/T--NCTU_Formosa--Ent_96_2.png" class="bac"> | |
| − | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599013" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z-Intein-CBD(BBa_K2599013)</p></a> | |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/8/8b/T--NCTU_Formosa--Lac_2.png" class="bac"> |
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599014" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q-Intein-CBD(BBa_K2599014)</p></a> |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/1/13/T--NCTU_Formosa--Leu_2.png" class="bac"> |
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a> |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" > |
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part: | + | |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | |
| − | <a href="http://parts.igem.org/wiki/index.php?title=Part: | + | |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | |
| − | + | ||
| − | + | <div class="title_1"><p>Curcumin Bio-sensor</p></div> | |
| − | + | <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a> | |
| + | <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;"> | ||
| + | |||
| + | <div class="title_1"><p>Improvement</p></div> | ||
| + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;"> | ||
| + | </div> | ||
</div> | </div> | ||
| Line 226: | Line 228: | ||
{{NCTU_Formosa/Footer}} | {{NCTU_Formosa/Footer}} | ||
| + | {{NCTU_Formosa/Top_button}} | ||
Latest revision as of 02:58, 18 October 2018
Bacteriocins
Bovicin HJ50-Intein-CBD(BBa_K2599009)
Enterocin B-Intein-CBD(BBa_K2599011)
Enterocin 96-Intein-CBD(BBa_K2599012)
Lacticin Z-Intein-CBD(BBa_K2599013)
Leucocyclicin Q-Intein-CBD(BBa_K2599014)
Durancin TW-49M-Intein-CBD(BBa_K2599015)
Curcumin Bio-sensor
GS Linker-αS1-casein(BBa_K2599017)
Improvement
Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)
