Difference between revisions of "Team:NCTU Formosa/Composite Part"

We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

Bacteriocins

Bovicin HJ50-Intein-CBD(BBa_K2599009)

Enterocin B-Intein-CBD(BBa_K2599011)

Enterocin 96-Intein-CBD(BBa_K2599012)

Lacticin Z-Intein-CBD(BBa_K2599013)

Leucocyclicin Q-Intein-CBD(BBa_K2599014)

Durancin TW-49M-Intein-CBD(BBa_K2599015)

Curcumin Bio-sensor

GS Linker-αS1-casein(BBa_K2599017)

Improvement

Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)

Template

@@ Line 38: / Line 38: @@
        overflow: hidden;
        background-color: white;
+      padding-bottom: 150px;
      }
@@ Line 53: / Line 54: @@
      .text{
-       font-size: 4vmin;
+       font-size: 3vmin;
-       width: 50%;
+       width: 70%;
        position: relative;
-       margin-left: 25%;
+       margin-left: 15%;
        margin-top: 40px;
        margin-bottom: 40px;
@@ Line 85: / Line 86: @@
        transition-duration: 0.4s;
        position: relative;
-       left: 25%;
+       left: 15%;
        margin: 30px;
      }
@@ Line 94: / Line 95: @@
      .bac {
+      width: 40%;
+      margin: 50px;
+      margin-left: 30%;
+      margin-bottom: 70px;
+    }
+    .curcumin {
        width: 40%;
        margin: 50px;
@@ Line 112: / Line 120: @@
      }
-    .basic_parts {
-      display: none;
-    }
      .part_button {
@@ Line 175: / Line 180: @@
      <div class="sec1">
        <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title">
-      <img src="https://static.igem.org/mediawiki/2018/5/5d/T--NCTU_Formosa--Parts_dec3.png" class="dec_1">
-      <div class="title_1"><p>Overview</p></div>
        <div class="text">
          <p>
-           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the eight antimicrobial peptides in <i>E. coli<i/>. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
+           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
            &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>.
          </p>
@@ Line 188: / Line 191: @@
        <div class="basic_parts">
          <div class="title_1"><p>Bacteriocins</p></div>
-        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599008" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin-Intein-CBD(BBa_K2599008)</p></a>
-        <img src="https://static.igem.org/mediawiki/2018/b/bf/T--NCTU_Formosa--Sub_2.png" class="bac">
          <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac">
-        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599010" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin A-Intein-CBD(BBa_K2599010)</p></a>
-        <img src="https://static.igem.org/mediawiki/2018/4/45/T--NCTU_Formosa--Ent_A_2.png" class="bac">
          <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac">
@@ Line 204: / Line 203: @@
          <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
+        <div class="title_1"><p>Curcumin Bio-sensor</p></div>
+        <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a>
+        <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;">
          <div class="title_1"><p>Improvement</p></div>
+        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a>
+        <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;">
        </div>
-      <img src="https://static.igem.org/mediawiki/2018/9/99/T--NCTU_Formosa--Parts_button.png" class="part_button" id="pb" onmouseup="mouseUp()">
-      <img src="https://static.igem.org/mediawiki/2018/6/61/T--NCTU_Formosa--Parts_dec4.png" class="dec_2">
      </div>
@@ Line 226: / Line 228: @@
 {{NCTU_Formosa/Footer}}
+{{NCTU_Formosa/Top_button}}