We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
Difference between revisions of "Team:NCTU Formosa/Composite Part"
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<p> | <p> | ||
| − | We tried to express the | + | We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br> |
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>. | The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>. | ||
</p> | </p> | ||
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<div class="basic_parts"> | <div class="basic_parts"> | ||
<div class="title_1"><p>Bacteriocins</p></div> | <div class="title_1"><p>Bacteriocins</p></div> | ||
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| − | |||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a> | ||
<img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac"> | <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac"> | ||
| − | |||
| − | |||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a> | ||
<img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac"> | <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac"> | ||
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a> | ||
<img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" > | <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" > | ||
| + | |||
| + | <div class="title_1"><p>Curcumin Bio-sensor</p></div> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;"> | ||
<div class="title_1"><p>Improvement</p></div> | <div class="title_1"><p>Improvement</p></div> | ||
| + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;"> | ||
</div> | </div> | ||
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{{NCTU_Formosa/Footer}} | {{NCTU_Formosa/Footer}} | ||
| + | {{NCTU_Formosa/Top_button}} | ||
Latest revision as of 02:58, 18 October 2018
Bacteriocins
Bovicin HJ50-Intein-CBD(BBa_K2599009)
Enterocin B-Intein-CBD(BBa_K2599011)
Enterocin 96-Intein-CBD(BBa_K2599012)
Lacticin Z-Intein-CBD(BBa_K2599013)
Leucocyclicin Q-Intein-CBD(BBa_K2599014)
Durancin TW-49M-Intein-CBD(BBa_K2599015)
Curcumin Bio-sensor
GS Linker-αS1-casein(BBa_K2599017)
Improvement
Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)
