We tried to express the six antimicrobial peptides by E. coli. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
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− | {{NCTU_Formosa}} | + | {{NCTU_Formosa/test}} |
<html> | <html> | ||
+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <title>Composite Parts</title> | ||
+ | <script src="jQueryAssets/jquery-1.11.1.min.js"></script> | ||
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− | < | + | <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.2.1/jquery.min.js"></script> |
− | + | <script> | |
− | + | $(document).ready(function(){ | |
− | + | $(".part_button").click(function(){ | |
− | </ | + | $(".basic_parts").slideToggle(); |
+ | }); | ||
+ | }); | ||
+ | </script> | ||
+ | <style> | ||
− | |||
+ | #globalWrapper{ | ||
+ | padding-bottom: 0 !important; | ||
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+ | body{ | ||
+ | font-size: 0; | ||
+ | margin: 0; | ||
+ | padding: 0 !important; | ||
+ | width: 100%; | ||
+ | font-family: Levenim MT; | ||
+ | } | ||
+ | .wrapper{ | ||
+ | width: 100%; | ||
+ | overflow: hidden; | ||
+ | background-color: white; | ||
+ | padding-bottom: 150px; | ||
+ | } | ||
+ | .cover{ | ||
+ | width: 100%; | ||
+ | } | ||
− | |||
− | |||
+ | .title{ | ||
+ | font-size: 7vmin; | ||
+ | margin: 50px; | ||
+ | margin-top: 0px; | ||
+ | text-align: center; | ||
+ | } | ||
− | + | .text{ | |
− | + | font-size: 3vmin; | |
− | + | width: 70%; | |
+ | position: relative; | ||
+ | margin-left: 15%; | ||
+ | margin-top: 40px; | ||
+ | margin-bottom: 40px; | ||
+ | text-align: justify; | ||
+ | } | ||
− | + | p { | |
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+ | } | ||
− | + | .title_1 { | |
− | + | font-size: 7vmin; | |
− | + | margin: 50px; | |
− | + | margin-top: 70px; | |
− | + | text-align: center; | |
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+ | .basic_title { | ||
+ | width: 35%; | ||
+ | margin-left: 32.5%; | ||
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+ | left: 15%; | ||
+ | margin: 30px; | ||
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− | + | .name:hover { | |
− | + | color: rgb(251, 185, 24) | |
+ | } | ||
− | + | .bac { | |
+ | width: 40%; | ||
+ | margin: 50px; | ||
+ | margin-left: 30%; | ||
+ | margin-bottom: 70px; | ||
+ | } | ||
− | + | .curcumin { | |
− | + | width: 40%; | |
+ | margin: 50px; | ||
+ | margin-left: 30%; | ||
+ | margin-bottom: 70px; | ||
+ | } | ||
− | </div> | + | .dec_1 { |
+ | width: 50%; | ||
+ | margin-left: 25%; | ||
+ | margin-top: 70px; | ||
+ | } | ||
+ | |||
+ | .dec_2 { | ||
+ | width: 50%; | ||
+ | margin-left: 25%; | ||
+ | margin-bottom: 150px; | ||
+ | } | ||
+ | |||
+ | |||
+ | .part_button { | ||
+ | width: 5%; | ||
+ | margin-left: 47.5%; | ||
+ | margin-bottom: 15px; | ||
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+ | |||
+ | .part_button:hover { | ||
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+ | .part_button:active { | ||
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+ | |||
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+ | |||
+ | </style> | ||
+ | |||
+ | <!-----------------------------------------------------------------------------> | ||
+ | |||
+ | <script> | ||
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+ | <!-----------------------------------------------------------------------------> | ||
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+ | <!-- Latex resourse code--> | ||
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+ | <!-----------------------------------------------------------------------------> | ||
+ | </head> | ||
+ | |||
+ | <body> | ||
+ | <div class="wrapper"> | ||
+ | <div class="banner"> | ||
+ | <img class="cover" src="https://static.igem.org/mediawiki/2018/6/63/T--NCTU_Formosa--Parts2.0.png"> | ||
+ | </div> | ||
+ | <div class="sec1"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/f/f8/T--NCTU_Formosa--Composite_parts.png" class="basic_title"> | ||
+ | <div class="text"> | ||
+ | <p> | ||
+ | We tried to express the six antimicrobial peptides by <i>E. coli</i>. These composite parts contain the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br> | ||
+ | The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <!---------------------------------------------------------------------> | ||
+ | <div class="basic_parts"> | ||
+ | <div class="title_1"><p>Bacteriocins</p></div> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac"> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac"> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599012" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin 96-Intein-CBD(BBa_K2599012)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/d/da/T--NCTU_Formosa--Ent_96_2.png" class="bac"> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599013" class="name" target="_blank" style="text-decoration: none;"><p>Lacticin Z-Intein-CBD(BBa_K2599013)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/8/8b/T--NCTU_Formosa--Lac_2.png" class="bac"> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599014" class="name" target="_blank" style="text-decoration: none;"><p>Leucocyclicin Q-Intein-CBD(BBa_K2599014)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/1/13/T--NCTU_Formosa--Leu_2.png" class="bac"> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" > | ||
+ | |||
+ | <div class="title_1"><p>Curcumin Bio-sensor</p></div> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1-casein(BBa_K2599017)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;"> | ||
+ | |||
+ | <div class="title_1"><p>Improvement</p></div> | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599016" class="name" target="_blank" style="text-decoration: none;"><p>Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)</p></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/b/bc/T--NCTU_Formosa--mprove_biobrick.png" class="curcumin" style="margin-bottom: 50px;"> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </body> | ||
+ | |||
+ | <script> | ||
+ | function mouseUp() { | ||
+ | document.getElementById("pb").style.transform = "rotate(180deg)"; | ||
+ | document.getElementById("pb").style.transform = "rotate(-180deg)"; | ||
+ | } | ||
+ | </script> | ||
</html> | </html> | ||
+ | |||
+ | {{NCTU_Formosa/Footer}} | ||
+ | {{NCTU_Formosa/Top_button}} |
Latest revision as of 02:58, 18 October 2018
Bacteriocins
Bovicin HJ50-Intein-CBD(BBa_K2599009)
Enterocin B-Intein-CBD(BBa_K2599011)
Enterocin 96-Intein-CBD(BBa_K2599012)
Lacticin Z-Intein-CBD(BBa_K2599013)
Leucocyclicin Q-Intein-CBD(BBa_K2599014)
Durancin TW-49M-Intein-CBD(BBa_K2599015)
Curcumin Bio-sensor
GS Linker-αS1-casein(BBa_K2599017)
Improvement
Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)