In our project, we believe that we can use the biostimulator to control the change of micribiota and make the soil
healthier. In order to verify this theory, we successively use the biostimulator in the soil where the turmeric was
planted in May, June and July. After sampling the NGS data of this three month, we classified the top 20 bacteria in those
data to get the function table at fig1. We can see that the ratio of plant growth-promoting bacteria (PGPB) is rising, and
the ratio of plant pathogenic bacteria is fluctuating in a small amount, which means that we can achieve the purpose of
raising good bacteria in the case of controlling the proportion of plant pathogenic bacteria by using the biostimulator.
Nitrifying bacteria and phosphate solubilizing bacteria rise. These bacteria produce chemical fertilizers, so their
increase can reduce the amount of fertilizer applied. The decline in pollution indicator bacteria indicates that the
proportion of pollutants in the soil components has decreased. That is, we have not applied pollutants such as pesticides,
herbicides etc., and the soil is slowly repairing itself over time. Finally, the data shows that the denitrifying bacteria
decline. Denitrifying bacteria can represent the nitrate content, and the high nitrate content will cause environmental
pollution.
All information shows us that every month after spreading the biostimulator, the soil gets healthier. It is worth that the
evenness of this three months is improving. Therefore, we confirmed that biostimulator can control bacterial changes and
make the soil healthier, and evenness can be used as a criterion for segmental soil health.