Difference between revisions of "Team:NEU China B"

 
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{{NEU_China_B}}
 
{{NEU_China_B}}
 
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Project Description
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$("#base_page").attr("width", c.parentElement.clientWidth);
−
Engineered probiotics alleviate the inflammatory bowel disease and prevent colorectal cancer
+
−
Due to the popularity of fast food and accelerated urbanization nowadays, the number of patients with inflammatory bowel disease(IBD) is rising in Asia. In addition, patients with IBD have an increased risk of developing colorectal cancer(CRC). Therefore, NEU China A aim to design a biological system against IBD and potential CRC by using engineered probiotics this year. To put out the fire in the gut, E. coli Nissle 1917 is selected as our firemen. We empower it with an anti-inflammatory device, which includes a sensor to detect the inflammatory signal, an amplifier to enhance the work ability through positive-feedback loop and an effector to secret anti-inflammatory part. Besides, we should watch out the bad guys such as cancer cells or invasive bacterial. So, we choose Saccharomyces boulardii to be our policemen. It is armed with a constitutively expressed myrosinase to turn the glucosinolates, a natural component of cruciferous vegetables, to sulphoraphane which is an organic molecule with known anticancer activity. We also equip our E. coli with this module since the sulphoraphane can inhibit the oxidative stress. In other hand, to restrict the bacterial invasion, the yeast is made to produce Lypd8, a protein represses the move of bacterial by binding to the flagella. Moreover, we decide to use a cold shock kill switch to avoid the escape of our chassis to the environment and more supporting modules will be added if necessary. We hope these modules will incorporate into a functional system eventually. Integrating probiotic therapy with synthetic biology, we envision that the collaboration between the firemen and the policemen will greatly help us to overcome the tough situation in the patient’s gut.
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<div class="column full_size" >
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var w1 = "https://static.igem.org/mediawiki/2018/a/a8/T--NEU_China_B--w1.png";
−
<h1> Welcome to iGEM 2018! </h1>
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var w2 = "https://static.igem.org/mediawiki/2018/f/f9/T--NEU_China_B--w2.png";
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
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−
<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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−
<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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−
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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</ul>
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−
<div class="column full_size" >
+
imgWarpper1.img = img1;
−
<h3> Styling your wiki </h3>
+
imgWarpper2.img = img2;
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
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−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<div class="clear extra_space"></div>
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var img2_x = x_e * Math.cos(t2 * 180 * 2) + basex;
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−
<div class="column third_size" >
+
var img4_x = x_e * Math.cos(t4 * 180 * 2) + basex;
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 +
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−
<h3> Uploading pictures and files </h3>
 
−
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
 
  
 +
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 +
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−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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−
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−
UPLOAD FILES
+
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<div class="column third_size" >
+
c.addEventListener('click', function (event) {
−
<h3> Wiki template information </h3>
+
var x = event.clientX - c.getBoundingClientRect().left;
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
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 +
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 +
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<div class="text-center abstract">
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<h2>
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Abstract
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</h2>
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<hr>
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The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing.
  
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<h3> Editing your wiki </h3>
 
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
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EDIT PAGE
 
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<img height=20 src="https://static.igem.org/mediawiki/2018/5/52/T--NEU_China_B--tb3.png" alt="" class="sr">
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<img height=20 src="https://static.igem.org/mediawiki/2018/4/48/T--NEU_China_B--tb4.png" alt="" class="src">
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<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/b/be/T--NEU_China_B--tb5.png/799px-T--NEU_China_B--tb5.png"
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<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/d/d9/T--NEU_China_B--tb7.png/320px-T--NEU_China_B--tb7.png"
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<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/4/4a/T--NEU_China_B--tb8.png/320px-T--NEU_China_B--tb8.png"
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<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/4/47/T--NEU_China_B--tb9.png/799px-T--NEU_China_B--tb9.png"
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<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/2/2d/T--NEU_China_B--tb10.png/202px-T--NEU_China_B--tb10.png"
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<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/b/be/T--NEU_China_B--tb5.png/320px-T--NEU_China_B--tb5.png"
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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<div class="column third_size">
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 03:39, 18 October 2018

Ruby - Responsive Corporate Tempalte

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Abstract

The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing.