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{{NCKU_Tainan/header}} {{NCKU_Tainan/navbar}} {{NCKU_Tainan/style}}
  
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            <h1 class="head">Collaborations</h1>
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        <div class="righttitle">
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            <h6 class="subtitle">United We Stand</h6>
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                <div id="sidelist" class="list-group">
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                    <a class="list-group-item list-group-item-action" href="#UESTC-China">UESTC-China</a>
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                    <a class="list-group-item list-group-item-action" href="#Tec-Monterrey">Tec-Monterrey</a>
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                            <div id="UESTC-China">
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                                <h3><a class="link" href="https://2018.igem.org/Team:UESTC-China">UESTC-China</a></h3>
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                                <div class="greenbold" id="centerimg">
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                                    <img class="contentimg" src="https://static.igem.org/mediawiki/2018/f/ff/T--NCKU_Tainan--UESTC_collabphoto.png">
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                                </br></br>
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                                <p class="pcontent">
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                                    During the time we designed a flow chart for our CO<sub>2</sub> utilization system for
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                                    application. We were lucky to find our friend, <a class="link" href="https://2018.igem.org/Team:UESTC-China">UESTC-China</a>, to work together and
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                                    end up building a completely eco-friendly system with negative carbon footprint.
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                                </p></br>
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                                <div id="pt">
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                                    </br>
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                                    <p class="pcontent">
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                                        We tried to figure out another way in carbon utilization as an
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                                        contribution of carbon reduction and save energy. However, we face some problem
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                                        when we tried to apply our Engineering <i>E. coli</i> into industrial scale. Cost
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                                        evaluation and carbon footprint are big challenges
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                                        When we were designing the whole CO<sub>2</sub> utilization system. We were delighted when
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                                        we found out someone who will be able to resolve our problems. UESTC-China mainly
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                                        focused on degrading straw into useful raw materials, including glucose, xylose,
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                                        ferule acid, and lignin. In addition, they aimed to convert glucose into butanol
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                                        and hydrogen. As xylose is the main carbon source for our
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                                        engineered <i>E. coli</i>, we came up with a collaboration idea by integrating our
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                                        system at the downstream of their system. Xylose is one of the side product
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                                        produced during straw degradation, it can be extracted and utilized in our
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                                        system. Concerning the synthesis of xylose may produce greenhouse gas and
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                                        chemicals, we would like to obtain xylose produced via biological method.
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                                    </p></br>
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                                </div>
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                                </div>
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                                    </br>
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                                    <p class="pcontent">
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                                        Through UESTC system, xylose will be produced in their fermentation broth as well as
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                                        other three raw material. In order to calculate the amount of xylose production,
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                                        they tested the ferulic acid which yield by gas chromatography (GC) after 12 hours.
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                                        Basically, ferulic acid cover for 1.15% of straw.
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                                        Then, the enzyme activity of ferulic acid degradation can be calculated.
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                                        Next, we assume that the rate of xylan decomposition is the same
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                                        as that of ferulic acid while 30% of xlyan composed of straw.
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                                        Finally, through derivation, we can get 0.0348 kg xylose from 1 kg straw.
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                                        The detail progress of calculation was listed below.
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                                    </p>
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                                    <br>
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                                    <p class="pcontent">
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                                        Sample :20ml, positive control and 0.3g corn straw was added,
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                                        Abs600 was not certain because we had added straw.
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                                    </p>
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                                    <p class="pcontent">Ferulic acid by GC : 0.53 mg / L</p>
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                                    <p class="pcontent">Ferulic acid in straw:0.3 g * 1.15% = 0.345 mg</p>
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                                    <p class="pcontent">Ferulic acid degradation rate:(0.53 * 20*10<sup>-3</sup>)/0.345 = 10.6%</p>
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                                    <p class="pcontent">Xylose in 1kg straw : 1kg * 30% * 10.6% = 0.0348 kg</p>
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                                    <br>
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                                    <p class="pcontent">
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                                        With NCKU system, <i>E. coli</i> consumed xylose to uptake CO<sub>2</sub>.
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                                        The xylose consumption was 23.864 kg to uptake 1kg of CO<sub>2</sub>.
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                                        In conclusion, every 685 kg straw can be used to capture 1 kg CO<sub>2</sub>.
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                                        In other words, capturing 1% annual CO<sub>2</sub> emission of
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                                        small and medium enterprise, which is 20 tons CO<sub>2</sub> require 16 tons straw. 
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                                    </p>
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                                    <p class="pcontent">
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                                        Straw was a biowaste in agriculture while it wasn't easy to decompose.
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                                        UESTC-China solved this problem with synthetic biology successfully.
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                                        Through our collaboration, they can not only produce bioenergy, but have
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                                        another powerful application in CO<sub>2</sub> emission problem. NCKU-Tainan provided them
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                                        a valuable and also applicable way and helped them design a draft of device.
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                                        The xylose source from straw was really important to <i>E. coli</i> CO<sub>2</sub> utilization system
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                                        which changed the positive carbon footprint into negative carbon footprint.
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                                        CO<sub>2</sub> emission problems is a big issue in the world. The main solution can be
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                                        separated into two part, CO<sub>2</sub> downstream processing and Alternative Energy. Both
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                                        NCKU-Tainan and UESTC-China contribute to this serious issue and our
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                                        collaboration could solve both the energy problem and environment problem.
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                                    </p></br>
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                                </div>
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                            </div>
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                            <div id="Tec-Monterrey">
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                                <h3><a class="link" href="https://2018.igem.org/Team:Tec-Monterrey">Tec-Monterrey</a></h3>
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                                </br></br>
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                                <div id="pt">
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                                    </br>
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<div id="centerimg">
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                                        <img class="col-12" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tec-Monterrey--Page_Loader.gif">
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                                </div>
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                                    <p class="pcontent">
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                                        We have a video meeting with <a class="link" href="https://2018.igem.org/Team:Tec-Monterrey">Tec-Monterrey</a>. After introducing our project to
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                                        them, they had pointed out some aspects that we need to consider. They reminded
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                                        us to think about our end product which can make our project more complete.
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                                    </p></br>
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                                </div>
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                                <div id="pt">
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                                    </br>
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                                    <p class="pcontent">
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                                        During the meeting, we came up with the idea to collaborate with each other.
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                                        Their project has two parts. The main part is E-coding, which use <i>E. coli</i> to
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                                        store data. Another part is to sense the pollutant in river of Mexico, which is
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                                        the part that we could link our work. We had constructed a pH sensing biobrick
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                                        which express fluorescence under low pH condition, they suggest us to
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                                        incorporate our pH sensing construct in their pollutant sensing system, making
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                                        their system become more complete. Therefore, we shared our plasmid map and
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                                        construction protocol with them, which they can be used to construct the
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                                        biobrick in the future.
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                                    </p></br>
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                                </div>
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                                <div id="pt">
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                                    </br>
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                                    <p class="pcontent">
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                                        Also, we found out that the new combined sensing system can be applied in our
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                                        bioreactor as they aim to sense pollutants such as nitrate, phosphate or heavy
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                                        metals. This will be a useful alert system for our bioreactor since the flue
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                                        gas may contain pollutants especially heavy metals that are highly toxic to the
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                                        <i>E. coli</i>.
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                                    </p></br>
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                                </div>
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                                <div id="pt">
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                                    </br>
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                                    <p class="pcontent">
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                                        Through this collaboration, we not only combined two sensing system, expanding
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                                        its application field, but we also found a more complete sensing system for our
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                                        device.
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                                    </p></br>
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                                </div>
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                            </div>
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                            </br></br></br></br></br></br>
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Latest revision as of 03:52, 18 October 2018

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