Difference between revisions of "Team:NCKU Tainan/Collaborations"

 
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         <h1 class="head4">Collaborations</h1>
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         <div class="headstyle">
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            <h1 class="head">Collaborations</h1>
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        </div>
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        <div class="righttitle">
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            <h6 class="subtitle">United We Stand</h6>
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                     <a class="list-group-item list-group-item-action" href="#UESTC-China">UESTC-China</a>
 
                     <a class="list-group-item list-group-item-action" href="#UESTC-China">UESTC-China</a>
 
                     <a class="list-group-item list-group-item-action" href="#Tec-Monterrey">Tec-Monterrey</a>
 
                     <a class="list-group-item list-group-item-action" href="#Tec-Monterrey">Tec-Monterrey</a>
                    <a class="list-group-item list-group-item-action" href="#NYMU">NYMU</a>
 
                    <a class="list-group-item list-group-item-action" href="#NCTU_Formosa">NCTU_Formosa</a>
 
 
                     <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x"
 
                     <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x"
 
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                         <div class="container">
 
                         <div class="container">
  
 
                             <div id="UESTC-China">
 
                             <div id="UESTC-China">
                                 </br></br></br></br>
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                                 <h3><a class="link" href="https://2018.igem.org/Team:UESTC-China">UESTC-China</a></h3>
                                 <h3>UESTC-China</h3>
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                                 <div class="greenbold" id="centerimg">
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                                    <img class="contentimg" src="https://static.igem.org/mediawiki/2018/f/ff/T--NCKU_Tainan--UESTC_collabphoto.png">
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                                </div>
 
                                 </br></br>
 
                                 </br></br>
 
                                 <p class="pcontent">
 
                                 <p class="pcontent">
                                     During the time we designed a flow chart for our CO2 utilization system for
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                                     During the time we designed a flow chart for our CO<sub>2</sub> utilization system for
                                     application, We were lucky to find our friend, UESTC-China, to work together and
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                                     application. We were lucky to find our friend, <a class="link" href="https://2018.igem.org/Team:UESTC-China">UESTC-China</a>, to work together and
 
                                     end up building a completely eco-friendly system with negative carbon footprint.
 
                                     end up building a completely eco-friendly system with negative carbon footprint.
 
                                 </p></br>
 
                                 </p></br>
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                                     </br>
 
                                     </br>
 
                                     <p class="pcontent">
 
                                     <p class="pcontent">
                                         Our project try to figure out another way in carbon utilization as an
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                                         We tried to figure out another way in carbon utilization as an
 
                                         contribution of carbon reduction and save energy. However, we face some problem
 
                                         contribution of carbon reduction and save energy. However, we face some problem
                                         when we try to apply our Engineering E. coli into industrial scale. Cost
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                                         when we tried to apply our Engineering <i>E. coli</i> into industrial scale. Cost
 
                                         evaluation and carbon footprint are big challenges
 
                                         evaluation and carbon footprint are big challenges
                                         When we were designing the whole CO2 utilization system. We were delighted when
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                                         When we were designing the whole CO<sub>2</sub> utilization system. We were delighted when
                                         we found out someone who will be able to resolve our problems. UESTC-China, an
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                                         we found out someone who will be able to resolve our problems. UESTC-China mainly
                                         iGEM team that focus on degrading straw into useful raw materials, which are
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                                         focused on degrading straw into useful raw materials, including glucose, xylose,  
                                        glucose, xylose, ferule acid, and lignin. In addition, they aimed to convert
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                                        ferule acid, and lignin. In addition, they aimed to convert glucose into butanol  
                                        glucose into butanol and hydrogen. As xylose is the main carbon source for our
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                                        and hydrogen. As xylose is the main carbon source for our
                                         engineered E. coli, we came up with a collaboration idea by integrating our
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                                         engineered <i>E. coli</i>, we came up with a collaboration idea by integrating our
 
                                         system at the downstream of their system. Xylose is one of the side product
 
                                         system at the downstream of their system. Xylose is one of the side product
 
                                         produced during straw degradation, it can be extracted and utilized in our
 
                                         produced during straw degradation, it can be extracted and utilized in our
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                                     </p></br>
 
                                     </p></br>
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                                </div>
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                                <div id="centerimg">
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                                        <img class="col-12" src="https://static.igem.org/mediawiki/2018/6/64/T--NCKU_Tainan--42891130_1999520940106016_5974534868648853504_n.png">
 
                                 </div>
 
                                 </div>
  
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                                     </br>
 
                                     </br>
 
                                     <p class="pcontent">
 
                                     <p class="pcontent">
                                         Straw was a bio waste in agriculture while it wasn't easy to decompose.
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                                        Through UESTC system, xylose will be produced in their fermentation broth as well as
                                         UESTC-China's work successfully solved this problem with synthetic biology.
+
                                        other three raw material. In order to calculate the amount of xylose production,
 +
                                        they tested the ferulic acid which yield by gas chromatography (GC) after 12 hours.
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                                        Basically, ferulic acid cover for 1.15% of straw.
 +
                                        Then, the enzyme activity of ferulic acid degradation can be calculated.
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                                        Next, we assume that the rate of xylan decomposition is the same
 +
                                        as that of ferulic acid while 30% of xlyan composed of straw.
 +
                                        Finally, through derivation, we can get 0.0348 kg xylose from 1 kg straw.
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                                        The detail progress of calculation was listed below.
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                                    </p>
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                                    <br>
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                                    <p class="pcontent">
 +
                                        Sample :20ml, positive control and 0.3g corn straw was added,
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                                        Abs600 was not certain because we had added straw.
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                                    </p>
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                                    <p class="pcontent">Ferulic acid by GC : 0.53 mg / L</p>
 +
                                    <p class="pcontent">Ferulic acid in straw:0.3 g * 1.15% = 0.345 mg</p>
 +
                                    <p class="pcontent">Ferulic acid degradation rate:(0.53 * 20*10<sup>-3</sup>)/0.345 = 10.6%</p>
 +
                                    <p class="pcontent">Xylose in 1kg straw : 1kg * 30% * 10.6% = 0.0348 kg</p>
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                                    <br>
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                                    <p class="pcontent">
 +
                                        With NCKU system, <i>E. coli</i> consumed xylose to uptake CO<sub>2</sub>.
 +
                                        The xylose consumption was 23.864 kg to uptake 1kg of CO<sub>2</sub>.
 +
                                        In conclusion, every 685 kg straw can be used to capture 1 kg CO<sub>2</sub>.
 +
                                        In other words, capturing 1% annual CO<sub>2</sub> emission of
 +
                                        small and medium enterprise, which is 20 tons CO<sub>2</sub> require 16 tons straw. 
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                                    </p>
 +
                                    <p class="pcontent">
 +
                                         Straw was a biowaste in agriculture while it wasn't easy to decompose.
 +
                                         UESTC-China solved this problem with synthetic biology successfully.
 
                                         Through our collaboration, they can not only produce bioenergy, but have
 
                                         Through our collaboration, they can not only produce bioenergy, but have
                                         another powerful application in CO2 emission problem. NCKU-Tainan provided them
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                                         another powerful application in CO<sub>2</sub> emission problem. NCKU-Tainan provided them
                                         a valuable application way and helped them design a draft of device. The xylose
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                                         a valuable and also applicable way and helped them design a draft of device.  
                                         source from straw was really important to E. coli co2 utilization system which
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                                         The xylose source from straw was really important to <i>E. coli</i> CO<sub>2</sub> utilization system  
                                         changed the positive carbon footprint into negative carbon footprint.
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                                         which changed the positive carbon footprint into negative carbon footprint.
                                         CO2 emission problems is a big issue in the world. The main solution can be
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                                         CO<sub>2</sub> emission problems is a big issue in the world. The main solution can be
                                         separated into two part, CO2 downstream processing and Alternative Energy. Both
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                                         separated into two part, CO<sub>2</sub> downstream processing and Alternative Energy. Both
 
                                         NCKU-Tainan and UESTC-China contribute to this serious issue and our
 
                                         NCKU-Tainan and UESTC-China contribute to this serious issue and our
 
                                         collaboration could solve both the energy problem and environment problem.
 
                                         collaboration could solve both the energy problem and environment problem.
 
                                     </p></br>
 
                                     </p></br>
 
                                 </div>
 
                                 </div>
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                             </div>
 
                             </div>
  
 
                             <div id="Tec-Monterrey">
 
                             <div id="Tec-Monterrey">
                                 </br></br></br></br>
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                                 <h3><a class="link" href="https://2018.igem.org/Team:Tec-Monterrey">Tec-Monterrey</a></h3>
                                <h3>Tec-Monterrey</h3>
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                                 </br></br>
 
                                 </br></br>
  
 
                                 <div id="pt">
 
                                 <div id="pt">
 
                                     </br>
 
                                     </br>
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<div id="centerimg">
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                                        <img class="col-12" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tec-Monterrey--Page_Loader.gif">
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                                </div>
 
                                     <p class="pcontent">
 
                                     <p class="pcontent">
                                         We have a video meeting with Tec-Monterrey. After introducing our project to
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                                         We have a video meeting with <a class="link" href="https://2018.igem.org/Team:Tec-Monterrey">Tec-Monterrey</a>. After introducing our project to
                                         them, they had pointed out some aspects that we need to consider. They remind
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                                         them, they had pointed out some aspects that we need to consider. They reminded
 
                                         us to think about our end product which can make our project more complete.
 
                                         us to think about our end product which can make our project more complete.
  
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                                     <p class="pcontent">
 
                                     <p class="pcontent">
 
                                         During the meeting, we came up with the idea to collaborate with each other.
 
                                         During the meeting, we came up with the idea to collaborate with each other.
                                         Their project has two part. The main part is E-coding, which use E. coli to
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                                         Their project has two parts. The main part is E-coding, which use <i>E. coli</i> to
 
                                         store data. Another part is to sense the pollutant in river of Mexico, which is
 
                                         store data. Another part is to sense the pollutant in river of Mexico, which is
 
                                         the part that we could link our work. We had constructed a pH sensing biobrick
 
                                         the part that we could link our work. We had constructed a pH sensing biobrick
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                                         metals. This will be a useful alert system for our bioreactor since the flue
 
                                         metals. This will be a useful alert system for our bioreactor since the flue
 
                                         gas may contain pollutants especially heavy metals that are highly toxic to the
 
                                         gas may contain pollutants especially heavy metals that are highly toxic to the
                                         E. coli.
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                                         <i>E. coli</i>.
 
                                     </p></br>
 
                                     </p></br>
 
                                 </div>
 
                                 </div>
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                                     </p></br>
 
                                     </p></br>
                                </div>
 
 
                            </div>
 
 
                            <div id="NYMU">
 
                                </br></br></br></br>
 
                                <h3>NYMU</h3>
 
                                </br></br>
 
 
                                <div id="pt">
 
                                    </br>
 
                                    <p class="pcontent">
 
                                        National Yang Ming Medical University (NYMU) iGEM team gave us a couple of
 
                                        comments after our presentation at the Asia Pacific Conference. First, NYMU
 
                                        pointed out that the production of xylose, a five carbon sugar we added in our
 
                                        cell culture medium consumes energy and hence contributes to the release of
 
                                        carbon dioxide. The release of carbon dioxide contradicts our effort in
 
                                        tackling the industrial CO2 issues. They further suggested us to convince our
 
                                        audience by proving our system captures more carbon dioxide than the carbon
 
                                        dioxide we produced. Second, they listed a couple of facts that we have to
 
                                        consider in calculating the cost of our system, especially the environmental
 
                                        costs that were easily neglected.
 
                                    </p></br>
 
                                </div>
 
 
                                <div id="pt">
 
                                    </br>
 
                                    <p class="pcontent">
 
                                        In return, we organized our comments and suggestions after watching their
 
                                        presentation before sending it to them via Facebook messenger.
 
                                    </p></br>
 
                                </div>
 
 
                            </div>
 
 
                            <div id="NCTU_Formosa">
 
                                </br></br></br></br>
 
                                <h3>NCTU_Formosa</h3>
 
                                </br></br>
 
 
                                <div id="pt">
 
                                    </br>
 
                                    <p class="pcontent">
 
                                        During 2018 iGEM 6th Asia and Pacific conference, we have more communication
 
                                        with National Chiao Tung University. Particularly, on the night before our
 
                                        present day, we invited the NCTU to help us listen to our present content. NCTU
 
                                        tell us about our problems, including the status of our present, the
 
                                        presentation of ppt, and most importantly, the questions of the experienment
 
                                        content.
 
                                    </p></br>
 
                                </div>
 
 
                                <div id="pt">
 
                                    </br>
 
                                    <p class="pcontent">
 
                                        NCTU listened very carefully to our present. We recorded and collected all
 
                                        their problems on the night. After returning to the dormitory, we discussed the
 
                                        appropriate responses to these questions with other teammates in Tainan.
 
                                    </p></br>
 
                                </div>
 
 
                                <div id="pt">
 
                                    </br>
 
                                    <p class="pcontent">
 
                                        Through rehearsal with NCTU on the night before our present, we have more
 
                                        direction in preparing for the formal present day. When we received the problem
 
                                        on the spot, we had more preparations. In this rehearsal, we are very grateful
 
                                        to NCTU's help. It also wake up our understanding of our project once again,
 
                                        and how to better interpret and explain our project content.
 
 
                                 </div>
 
                                 </div>
  
Line 208: Line 181:
 
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Latest revision as of 03:52, 18 October 2018

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