Difference between revisions of "Team:NCKU Tainan/Collaborations"

 
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         <h1 class="head4">Collaborations</h1>
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            <h1 class="head">Collaborations</h1>
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        </div>
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        <div class="righttitle">
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            <h6 class="subtitle">United We Stand</h6>
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         <div class="navbar-example">
 
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                     <a class="list-group-item list-group-item-action" href="#UESTC-China">UESTC-China</a>
 
                     <a class="list-group-item list-group-item-action" href="#UESTC-China">UESTC-China</a>
 
                     <a class="list-group-item list-group-item-action" href="#Tec-Monterrey">Tec-Monterrey</a>
 
                     <a class="list-group-item list-group-item-action" href="#Tec-Monterrey">Tec-Monterrey</a>
               
 
               
 
 
                     <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x"
 
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                             <div id="UESTC-China">
 
                             <div id="UESTC-China">
                                 </br></br></br></br>
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                                 <h3><a class="link" href="https://2018.igem.org/Team:UESTC-China">UESTC-China</a></h3>
                                 <h3>UESTC-China</h3>
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                                 <div class="greenbold" id="centerimg">
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                                    <img class="contentimg" src="https://static.igem.org/mediawiki/2018/f/ff/T--NCKU_Tainan--UESTC_collabphoto.png">
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                                </div>
 
                                 </br></br>
 
                                 </br></br>
 
                                 <p class="pcontent">
 
                                 <p class="pcontent">
                                     During the time we designed a flow chart for our CO2 utilization system for
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                                     During the time we designed a flow chart for our CO<sub>2</sub> utilization system for
                                     application, We were lucky to find our friend, UESTC-China, to work together and
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                                     application. We were lucky to find our friend, <a class="link" href="https://2018.igem.org/Team:UESTC-China">UESTC-China</a>, to work together and
 
                                     end up building a completely eco-friendly system with negative carbon footprint.
 
                                     end up building a completely eco-friendly system with negative carbon footprint.
 
                                 </p></br>
 
                                 </p></br>
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                                     </br>
 
                                     </br>
 
                                     <p class="pcontent">
 
                                     <p class="pcontent">
                                         Our project try to figure out another way in carbon utilization as an
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                                         We tried to figure out another way in carbon utilization as an
 
                                         contribution of carbon reduction and save energy. However, we face some problem
 
                                         contribution of carbon reduction and save energy. However, we face some problem
                                         when we try to apply our Engineering E. coli into industrial scale. Cost
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                                         when we tried to apply our Engineering <i>E. coli</i> into industrial scale. Cost
 
                                         evaluation and carbon footprint are big challenges
 
                                         evaluation and carbon footprint are big challenges
                                         When we were designing the whole CO2 utilization system. We were delighted when
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                                         When we were designing the whole CO<sub>2</sub> utilization system. We were delighted when
                                         we found out someone who will be able to resolve our problems. UESTC-China, an
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                                         we found out someone who will be able to resolve our problems. UESTC-China mainly
                                         iGEM team that focus on degrading straw into useful raw materials, which are
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                                         focused on degrading straw into useful raw materials, including glucose, xylose,  
                                        glucose, xylose, ferule acid, and lignin. In addition, they aimed to convert
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                                        ferule acid, and lignin. In addition, they aimed to convert glucose into butanol  
                                        glucose into butanol and hydrogen. As xylose is the main carbon source for our
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                                        and hydrogen. As xylose is the main carbon source for our
                                         engineered E. coli, we came up with a collaboration idea by integrating our
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                                         engineered <i>E. coli</i>, we came up with a collaboration idea by integrating our
 
                                         system at the downstream of their system. Xylose is one of the side product
 
                                         system at the downstream of their system. Xylose is one of the side product
 
                                         produced during straw degradation, it can be extracted and utilized in our
 
                                         produced during straw degradation, it can be extracted and utilized in our
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                                     </p></br>
 
                                     </p></br>
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                                        <img class="col-12" src="https://static.igem.org/mediawiki/2018/6/64/T--NCKU_Tainan--42891130_1999520940106016_5974534868648853504_n.png">
 
                                 </div>
 
                                 </div>
  
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                                     </br>
 
                                     </br>
 
                                     <p class="pcontent">
 
                                     <p class="pcontent">
                                         Straw was a bio waste in agriculture while it wasn't easy to decompose.
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                                        Through UESTC system, xylose will be produced in their fermentation broth as well as
                                         UESTC-China's work successfully solved this problem with synthetic biology.
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                                        other three raw material. In order to calculate the amount of xylose production,
 +
                                        they tested the ferulic acid which yield by gas chromatography (GC) after 12 hours.
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                                        Basically, ferulic acid cover for 1.15% of straw.
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                                        Then, the enzyme activity of ferulic acid degradation can be calculated.
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                                        Next, we assume that the rate of xylan decomposition is the same
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                                        as that of ferulic acid while 30% of xlyan composed of straw.
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                                        Finally, through derivation, we can get 0.0348 kg xylose from 1 kg straw.
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                                        The detail progress of calculation was listed below.
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                                    </p>
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                                    <br>
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                                    <p class="pcontent">
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                                        Sample :20ml, positive control and 0.3g corn straw was added,
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                                        Abs600 was not certain because we had added straw.
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                                    </p>
 +
                                    <p class="pcontent">Ferulic acid by GC : 0.53 mg / L</p>
 +
                                    <p class="pcontent">Ferulic acid in straw:0.3 g * 1.15% = 0.345 mg</p>
 +
                                    <p class="pcontent">Ferulic acid degradation rate:(0.53 * 20*10<sup>-3</sup>)/0.345 = 10.6%</p>
 +
                                    <p class="pcontent">Xylose in 1kg straw : 1kg * 30% * 10.6% = 0.0348 kg</p>
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                                    <br>
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                                    <p class="pcontent">
 +
                                        With NCKU system, <i>E. coli</i> consumed xylose to uptake CO<sub>2</sub>.
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                                        The xylose consumption was 23.864 kg to uptake 1kg of CO<sub>2</sub>.
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                                        In conclusion, every 685 kg straw can be used to capture 1 kg CO<sub>2</sub>.
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                                        In other words, capturing 1% annual CO<sub>2</sub> emission of
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                                        small and medium enterprise, which is 20 tons CO<sub>2</sub> require 16 tons straw. 
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                                    </p>
 +
                                    <p class="pcontent">
 +
                                         Straw was a biowaste in agriculture while it wasn't easy to decompose.
 +
                                         UESTC-China solved this problem with synthetic biology successfully.
 
                                         Through our collaboration, they can not only produce bioenergy, but have
 
                                         Through our collaboration, they can not only produce bioenergy, but have
                                         another powerful application in CO2 emission problem. NCKU-Tainan provided them
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                                         another powerful application in CO<sub>2</sub> emission problem. NCKU-Tainan provided them
                                         a valuable application way and helped them design a draft of device. The xylose
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                                         a valuable and also applicable way and helped them design a draft of device.  
                                         source from straw was really important to E. coli co2 utilization system which
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                                         The xylose source from straw was really important to <i>E. coli</i> CO<sub>2</sub> utilization system  
                                         changed the positive carbon footprint into negative carbon footprint.
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                                         which changed the positive carbon footprint into negative carbon footprint.
                                         CO2 emission problems is a big issue in the world. The main solution can be
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                                         CO<sub>2</sub> emission problems is a big issue in the world. The main solution can be
                                         separated into two part, CO2 downstream processing and Alternative Energy. Both
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                                         separated into two part, CO<sub>2</sub> downstream processing and Alternative Energy. Both
 
                                         NCKU-Tainan and UESTC-China contribute to this serious issue and our
 
                                         NCKU-Tainan and UESTC-China contribute to this serious issue and our
 
                                         collaboration could solve both the energy problem and environment problem.
 
                                         collaboration could solve both the energy problem and environment problem.
 
                                     </p></br>
 
                                     </p></br>
 
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                                 </div>
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                             </div>
 
                             </div>
  
 
                             <div id="Tec-Monterrey">
 
                             <div id="Tec-Monterrey">
                                 </br></br></br></br>
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                                 <h3><a class="link" href="https://2018.igem.org/Team:Tec-Monterrey">Tec-Monterrey</a></h3>
                                <h3>Tec-Monterrey</h3>
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                                 </br></br>
 
                                 </br></br>
  
 
                                 <div id="pt">
 
                                 <div id="pt">
 
                                     </br>
 
                                     </br>
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<div id="centerimg">
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                                        <img class="col-12" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tec-Monterrey--Page_Loader.gif">
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                                </div>
 
                                     <p class="pcontent">
 
                                     <p class="pcontent">
                                         We have a video meeting with Tec-Monterrey. After introducing our project to
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                                         We have a video meeting with <a class="link" href="https://2018.igem.org/Team:Tec-Monterrey">Tec-Monterrey</a>. After introducing our project to
                                         them, they had pointed out some aspects that we need to consider. They remind
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                                         them, they had pointed out some aspects that we need to consider. They reminded
 
                                         us to think about our end product which can make our project more complete.
 
                                         us to think about our end product which can make our project more complete.
  
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                                     <p class="pcontent">
 
                                     <p class="pcontent">
 
                                         During the meeting, we came up with the idea to collaborate with each other.
 
                                         During the meeting, we came up with the idea to collaborate with each other.
                                         Their project has two part. The main part is E-coding, which use E. coli to
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                                         Their project has two parts. The main part is E-coding, which use <i>E. coli</i> to
 
                                         store data. Another part is to sense the pollutant in river of Mexico, which is
 
                                         store data. Another part is to sense the pollutant in river of Mexico, which is
 
                                         the part that we could link our work. We had constructed a pH sensing biobrick
 
                                         the part that we could link our work. We had constructed a pH sensing biobrick
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                                         metals. This will be a useful alert system for our bioreactor since the flue
 
                                         metals. This will be a useful alert system for our bioreactor since the flue
 
                                         gas may contain pollutants especially heavy metals that are highly toxic to the
 
                                         gas may contain pollutants especially heavy metals that are highly toxic to the
                                         E. coli.
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                                         <i>E. coli</i>.
 
                                     </p></br>
 
                                     </p></br>
 
                                 </div>
 
                                 </div>
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                             </br></br></br></br></br></br>
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Latest revision as of 03:52, 18 October 2018

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