Difference between revisions of "Team:NCKU Tainan/Description"

 
(23 intermediate revisions by 4 users not shown)
Line 7: Line 7:
 
         <div class="container content">
 
         <div class="container content">
 
             <div class="headstyle">
 
             <div class="headstyle">
                 <h1 class="head">Background / Description</h1>
+
                 <h1 class="head">Description</h1>
 
             </div>
 
             </div>
 
             <div class="righttitle">
 
             <div class="righttitle">
                 <h6 class="subtitle">Ideas Come True</h6>
+
                 <h6 class="subtitle">The Story Begins Here</h6>
 
             </div>
 
             </div>
 
             <div class="navbar-example">
 
             <div class="navbar-example">
Line 28: Line 28:
 
                     <div class="col-10">
 
                     <div class="col-10">
 
                         <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example">
 
                         <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example">
                             <br>
+
                             <br><br>
 
                             <div class="container">
 
                             <div class="container">
 
                               <video class="video" controls="">
 
                               <video class="video" controls="">
Line 39: Line 39:
 
                                         <img class="smallimg" src="https://static.igem.org/mediawiki/2018/6/65/T--NCKU_Tainan--of_course.png" alt="of course">
 
                                         <img class="smallimg" src="https://static.igem.org/mediawiki/2018/6/65/T--NCKU_Tainan--of_course.png" alt="of course">
 
                                     </div>
 
                                     </div>
                                     <p class="pcontent">In order to reduce the concentration of carbon dioxide which is surrounding in environment,
+
                                     <p class="pcontent">2018 NCKU Tainan team strives to reduce the concentration of carbon dioxide  
                                         our team has developed a device, containing our engineered <i>E. coli</i> to fix the carbon dioxide
+
                                         (CO<sub>2</sub>) with the approach of synthetic biology.
                                        when it undergoes its native metabolic pathway.  
+
We constructed a carbon fixing <i>E. coli</i> by cloning enzymes from Calvin cycle into it. We measured the function of each enzyme in the pathway and proved that the engineered pathway can utilize carbon.
                                        Those three data, temperature, concentration of carbon dioxide,
+
                                         Our team has also designed a bioreactor. The bioreactor contains an air valve in which we can pump the CO<sub>2</sub>. The bioreactor is also monitored by sensors that will send the data to a cloud database. Combining IoT and synthetic biology, we believe that our device can be applied to industrial settings.  
                                         and pH value will be shown on the screen as we have developed an App for our user.  
+
                                         To promote our design as well as synthetic biology, we have discussed with numerous experts in various professions and visit the entrepreneurship that is willing to apply our design. We received countless feedbacks and improve our project. We believe that people should be aware of CO<sub>2</sub>. We also believe that our project can help the society to get one step closer towards a low carbon society.
                                         All of our ideas are to provide an another choice for public without any replacement as we are really longing for the low carbon society.
+
                                        Therefore, we use microorganism to ,'of course', reduce the carbon dioxide in the atmosphere.
+
 
                                     </p>
 
                                     </p>
 
                                    
 
                                    
Line 53: Line 51:
 
                                 <div id="Background">
 
                                 <div id="Background">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/8/82/T--NCKU_Tainan--background_background.png" alt="background">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/8/82/T--NCKU_Tainan--background_background.png" alt="background">
                                     <p class="pcontent">The issue of the greenhouse effect has always been a problem that needs to be solved
+
                                     <p class="pcontent">It is not exaggerated to say that us, human being, are at the crisis that stems from CO<sub>2</sub> since the industrial revolution in the 18<sup>th</sup> century.
                                        by not only some environmental protection section but to all human being  
+
                                        in the current society since the awareness of industrial evolution in the 18<sup>th</sup> century.
+
 
                                     </p>
 
                                     </p>
 
                                     <p class="pcontent">Greenhouse effect brings a lot of side effects to us.  
 
                                     <p class="pcontent">Greenhouse effect brings a lot of side effects to us.  
                                         For example, ice melting causes the rise of sea level while the land for terrestrial to live
+
                                         For example, glacier retreats, which contributes to the rise of sea level and the decrease of habitable land. Besides, the rise of temperature accelerates the chemical reaction in the sea, depleting the coral reef and lead to the unbalanced marine ecosystem. The frequency of natural disaster has drastically increased over the past few decades. If the global temperature rises 1 degree Celcius, one-third of the land will be lack of freshwater. 2 degrees Celcius, over millions of species will go extinct. 4 degrees Celcius, the glacier in the Northpole will melt completely.
                                        is getting lesser and lesser. Besides, the global temperature will increase resulting the rise of sea temperature,
+
                 
                                        and that rise of temperature will make the chemical reaction in the sea to be faster than usual
+
                                 
                                        depleting the coral reef so the ecosystem in the sea will become unbalance.  
+
                                        Furthermore, greenhouse effect will also be resulting the depletion of the ozone layer.  
+
                                        It means that living things will exposed under ultraviolet light and all the living things will live
+
                                        at a high mutation rate.  
+
                                        As a result, human will easily get the dermatitis disease than the past, and even have a high risk of skin cancer.
+
 
                                     </p>
 
                                     </p>
 
                                     <div class="centerimg">
 
                                     <div class="centerimg">
 
                                         <img class="gif" src="https://static.igem.org/mediawiki/2018/e/ec/T--NCKU_Tainan--background_WMO.gif" alt="WMO">
 
                                         <img class="gif" src="https://static.igem.org/mediawiki/2018/e/ec/T--NCKU_Tainan--background_WMO.gif" alt="WMO">
 
                                     </div>  
 
                                     </div>  
                                     <p class="pcontent">In conclusion, if we are still not going to take care of this,  
+
                                     <p class="pcontent">If we do not pay attention to this ongoing crisis,  
 
                                         we will face the risk of extinction as the situation is getting worse.  
 
                                         we will face the risk of extinction as the situation is getting worse.  
                                         As one of the members in global village, our team,  
+
                                         As one of the members of the global village, our team,  
                                         NCKU_Tainan thinks that we should enroll ourselves in movement that can help reduce the emission of  
+
                                         NCKU_Tainan thinks that we should enroll ourselves in reducing the emission of greenhouse gases (GHGs). This year, our team have designed a new way to utilize one of the GHGs,  
                                        greenhouse gaseous (GHGs). This year, our team designed a new way to catch one of the GHGs,  
+
                                         CO<sub>2</sub>, by using an advanced and modern technique nowadays, Synthetic Biology.  
                                         carbon dioxide (CO<sub>2</sub>) by using an advance and modern technique nowadays, Synthetic Biology.  
+
                                         Without replacing other ways, our team offers an alternative to utilize carbon dioxide.
                                         Without any replacement with other ways but to offer another way to public,
+
                                        the public could used it in different condition.
+
 
                                     </p>
 
                                     </p>
 
                                     <div class="borderstyle">
 
                                     <div class="borderstyle">
 
                                         <img class="bigimg" src="https://static.igem.org/mediawiki/2018/3/35/T--NCKU_Tainan--background_ccsu.png" alt="CCSU">
 
                                         <img class="bigimg" src="https://static.igem.org/mediawiki/2018/3/35/T--NCKU_Tainan--background_ccsu.png" alt="CCSU">
                                         <p class="pcontent">In order to reduce greenhouse gas emissions,  
+
                                         <p class="pcontent">With the aim of reducing GHGs emissions,  
                                             industry has long been committed to energy saving and carbon reduction in the process,
+
                                             the industry has developed various energy saving and carbon reducing manufacturing process.
                                             and carbon dioxide emissions in the factory are moving toward the development of Carbon dioxide Capture,
+
                                             CO<sub>2</sub> emissions in the factory are currently processed by the technology of Carbon Capture  
                                             Storage, and Utilization (CCSU) to reduce directly emits CO<sub>2</sub> into the atmosphere.
+
                                             Storage and Utilization (CCS & U). Development of CCS & U is a crucial factor in reducing directly CO<sub>2</sub> emission.
 
                                         </p>
 
                                         </p>
 
                                     </div>
 
                                     </div>
Line 90: Line 79:
 
                                 <div id="Bacteria">
 
                                 <div id="Bacteria">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5e/T--NCKU_Tainan--background_bacteria.png" alt="Bacteria">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5e/T--NCKU_Tainan--background_bacteria.png" alt="Bacteria">
                                     <p class="pcontent">After doing our research, we believed that it is possible to clone some gene fragments  
+
                                     <p class="pcontent">After doing some research, we cloned gene fragments  
                                         that can express PRK, CA and RuBisCO which is from a unicellular cyanobacteria strain,  
+
                                         that can express PRK, CA and Rubisco into <i>E. coli</i>. All of the genes are originated from a unicellular cyanobacteria strain,  
                                         <i>Synchococcus alongatus pcc 7942</i> and  <i>Synchococcus alongatus pcc 7002</i> into our model organism, <i>E. coli</i>.  
+
                                         <i>Synechococcus elongatus</i> PCC 7942 and  <i>Synechococcus elongatus</i> PCC 7002.  
                                         After cloning the three gene fragments into our <i>E. coli</i>,  
+
                                         After cloning three gene fragments into <i>E. coli</i>,  
                                         it could express those gene and make a new metabolic pathway that can accomplish carbon capture,  
+
                                         a new metabolic pathway that can utilize carbon dioxide was established,  
                                         subverting the cognition that only most of the plants could fix carbon  dioxide.
+
                                         subverting the cognition that only autotrophs could utilize CO<sub>2</sub>.
 
                                     </p>
 
                                     </p>
                                     <p class="pcontent">Firstly, <i>E. coli</i> will uptake xylose in the surrounding of its cultivation environment.  
+
                                     <p class="pcontent">Initially, <i>E. coli</i> will uptake xylose from the cultivation medium.  
                                         After that, xylose will be metabolized and converted into Ru5P by its own native metabolic pathway.  
+
                                         After that, xylose will be metabolized into Ru5P by its own native metabolic pathway.  
                                         Next, PRK will convert Ru5P into RuBP. Lastly, RuBP will react with carbon dioxide
+
                                         Next, PRK catalyzed the phosphorylation of Ru5P to RuBP. RuBP will be carboxylated into two molecules of 3-PGA. 3-PGA will be converted into a multifunctional intermediate, pyruvate.
                                        which fixed by RuBisCO converted into 3-PGA and 3-PGA will become a multifunctional intermediate, pyruvate.
+
 
                                     </p>
 
                                     </p>
 
                                 </div>
 
                                 </div>
 
                                 <div id="Device">
 
                                 <div id="Device">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/7/7a/T--NCKU_Tainan--background_device.png" alt="device">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/7/7a/T--NCKU_Tainan--background_device.png" alt="device">
                                     <p class="pcontent">In order to integrate with industrial IGCC system,  
+
                                     <p class="pcontent">In order to expand the scope of our project to be used in the field,  
                                         our team designed a device which divided into three main parts, a bioreactor, consisting a pH meter,  
+
                                         our team have designed a device to let our engineered <i>E. coli</i> to utilize CO<sub>2</sub> in real life. The device can be divided into three main parts. Our bioreactor consists of a pH meter,  
                                         a thermometer and a carbon dioxide sensor that could let our engineered <i>E. coli</i> to fix carbon dioxide.
+
                                         a thermometer and a CO<sub>2</sub> sensor, a nutrient tank,
                                        The second part and third part are a nutrient tank,  
+
                                         containing a fed-batch culture system and a collection tank to collect waste and product at the downstream of our device.
                                         containing a fed-batch culture system and a collection tank to collect waste and product at the end of the whole progress.
+
 
                                     </p>
 
                                     </p>
                                     <p class="pcontent">Next, in the consideration of allowing our users monitor the internal condition of our device  
+
                                     <p class="pcontent">Next, for the consideration to the real time internal condition monitor of our device, we have designed an App. No matter where you are, users could determine the progress of bioreactor with the immediate data of temperature,  
                                        conveniently, we have developed an App for them. By using this App, users could see the data of temperature,  
+
                                         CO<sub>2</sub> concentration and pH value. These data come from the sensors on our device which will be showed on the App through the database, uploaded by WI-FI module.
                                         carbon dioxide concentration and also pH value which are from our database, uploaded by WI-FI.
+
 
                                     </p>
 
                                     </p>
 
                                 </div>
 
                                 </div>
Line 119: Line 105:
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/3/39/T--NCKU_Tainan--background_hp.png" alt="hp">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/3/39/T--NCKU_Tainan--background_hp.png" alt="hp">
 
                                     <p class="pcontent">In the Integrated Human Practice, we improved our project from different activities in different aspects.  
 
                                     <p class="pcontent">In the Integrated Human Practice, we improved our project from different activities in different aspects.  
                                         We visited professors when we encountered difficulties, and they always give us corrections instantly.  
+
                                         We visited experts in many kinds of specialties when we encountered difficulties. Their instant feedback helped us walk in the right direction.  
                                         Since our project converge diversify fields, the more suggestions we had, the more integrity we propose.
+
                                         Besides, our project converges diversify fields, so the more suggestions we had, the more integrity we propose.
 
                                     </p>
 
                                     </p>
                                     <p class="pcontent">As for the appliance of our project, industrial interview is our primary concern.  
+
                                     <p class="pcontent">As for the application of our project, industrial interviews supports us to improve our project. Each time we held an interview with an enterprise, we get more suggestions and were able to meet their needs. One of the important concern of the enterprise is to maximize the benefit and reduce the cost simultaneously.  
                                        To satisfy enterprise's demand, we researched a lot and modified our project to make it better.  
+
                                         Besides, meeting up with other iGEMers built a strong friendship with those who also have a strong interest in synthetic biology. We also seek the opportunity to collaborate and create a win-win situation.
                                        What's more, after interviewing with enterprise,
+
                                        they suggested us to concern more about maximizing the benefit and making the lowest cost of our project simultaneously.  
+
                                         Meeting up with other iGEMers built a strong friendship with those who also have interest in synthetic biology.  
+
                                        We communicated with each other, figured out the possibility of collaboration, and created a win-win situation.
+
 
                                     </p>
 
                                     </p>
 
                                 </div>
 
                                 </div>
Line 134: Line 116:
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5f/T--NCKU_Tainan--background_education.png" alt="hp">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5f/T--NCKU_Tainan--background_education.png" alt="hp">
 
                                     <p class="pcontent">For the education and public engagement,  
 
                                     <p class="pcontent">For the education and public engagement,  
                                         we attempt to introduce synthetic biology to the students and public in a more interesting way.  
+
                                         we attempted to introduce synthetic biology to the students and public in a more interesting way.  
 
                                         The more people we affect, the more people we can cooperate.
 
                                         The more people we affect, the more people we can cooperate.
 
                                     </p>
 
                                     </p>
                                     <p class="pcontent">Different from normal course,  
+
                                     <p class="pcontent">Different from normal courses, we designed easy going and interesting experiments and app games for students and the public, which led them to become interested in iGEM. After these activities, their feedbacks helped us to understand their thoughts and we were able to adjust and improve our presentation for the next time.
                                        we design easy-going but interesting experiment and App game for students and public,  
+
                                    </p>
                                        which let them be fond of and involved in iGEM. After the activity,  
+
                                     <p class="pcontent">Furthermore, we participated in a festival held within our university NCKU as well at a medical conference within the medical school and operated our fan page to increase the exposure of our project, as well as synthetic biology and iGEM. Our fan page also aided in raising the awareness of iGEM in our school, which attracted more student to join us.
                                        their feedback help us to understand their thought.
+
                                        Then we can adjust in the next promotion we brought to the society. In addition,
+
                                        we also recognize where we do well and where we have to modify more to trigger more people be interested in iGEM.
+
                                    </p>
+
                                     <p class="pcontent">Besides, we joined festival in NCKU and medical conference,
+
                                        and operated our fan page to increase our project exposure, as well as synthetic biology and iGEM.  
+
                                        It also raises the discussion of iGEM in our school, which attracted more student to join us.
+
 
                                     </p>
 
                                     </p>
 
                                 </div>
 
                                 </div>

Latest revision as of 15:25, 3 November 2018

Description

The Story Begins Here
Follow us

Contact us

igem.ncku.tainan@gmail.com
No.1, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)