Difference between revisions of "Team:NCKU Tainan"

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~~<h3>Of CO<sub>2~~</~~sub>urse</h3>~~

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~~<h4>A biological approach to “off” CO<sub>2</sub> emission</h4>~~

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Ever since the 90s when concern over the impact of carbon emission on our environment was first raised, global-wide efforts in reducing emission have been met with mixed results. Just 2017 alone the global emission level grew by 1.4%. This year, iGEM NCKU_Tainan will design a device capable of piping CO<sub>2</sub> and convert such carbon source into biomass via intergrating a non-native Calvin-Benson-Bassham cycle into <i>E. coli</i>. “Of Course” is a biological approach to “off” CO<sub>2</sub> emission through the RuBisCO and PRK genes from <i>Synechococcus sp</i>, which encode for major enzymes involved in carbon fixation. Industrial gases will enter a pipe (inlet) at the bottom of a cylindrical container, flow through a ceramic nozzle and mix with liquids containing engineered <i>E. coli</i> that consumes CO<sub>2</sub>. Furthermore, CO<sub>2</sub> concentration will be determined by monitoring the corresponding change in pH using an asr (acid shock RNA) promoter containing <i>E. coli</i> with a sfGFP as reporter. Our ultimate goal is that this novel approach will reduce CO<sub>2</sub> in order to slow down global climate changes.

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