Difference between revisions of "Team:Ruia-Mumbai/Experiments"

 
(29 intermediate revisions by 2 users not shown)
Line 43: Line 43:
  
 
.centre {
 
.centre {
margin-top: 50px;
+
  margin-top: 50px;
margin-bottom: 50px;
+
  margin-bottom: 50px;
+
 
margin: auto;
+
  margin: auto;
border: 5px solid grey;
+
  border: 5px solid grey;
  
border-radius: 25px;
+
  border-radius: 25px;
width: 75%;
+
  width: 75%;
 
     color: white;
 
     color: white;
 
     padding-left:20px;
 
     padding-left:20px;
padding-right:20px;
+
  padding-right:20px;
  
padding-bottom: 100px;
+
  padding-bottom: 30px;
 
      
 
      
 
}
 
}
Line 77: Line 77:
 
.active, .accordion:hover {
 
.active, .accordion:hover {
 
     background-color: #ccc;
 
     background-color: #ccc;
 +
 
}
 
}
  
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.panel {
 
.panel {
+
 
 
     padding-right: 10px;
 
     padding-right: 10px;
 
    
 
    
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.pdf{
 
.pdf{
padding: 40px;
+
  padding: 40px;
margin: 0 auto;
+
  margin: 0 auto;
display: block;
+
  display: block;
height: 800px;
+
  height: 800px;
  
 
}
 
}
Line 146: Line 147:
  
 
}
 
}
 +
 +
  
 
input:focus + .month-picker-label, .month-picker-nav:focus {
 
input:focus + .month-picker-label, .month-picker-nav:focus {
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}
 
}
 +
 +
  
 
.month-picker-label, .month-picker-label:before {
 
.month-picker-label, .month-picker-label:before {
Line 186: Line 191:
 
input:checked + .month-picker-label {
 
input:checked + .month-picker-label {
 
color : red;
 
color : red;
border : solid red;
+
 
 
border-radius: 5px;
 
border-radius: 5px;
 
font-weight : bolder;
 
font-weight : bolder;
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}
 
}
  
 +
@media only screen and (max-width: 767px)
 +
{
 +
 +
.month-picker-label {
 +
  font-size: 12px;
 +
}
 +
 +
 +
.centre {
 +
width: 90%; 
 +
border: 0.1em solid grey; 
 +
 +
}
 +
 +
.pdf{
 +
 +
height: 15vh;
 +
padding : 20px;
 +
 +
}
 +
.accordion {
 +
    font-size: 0.8em;
 +
    padding :10px;
 +
}
 +
 +
.tabcontent{
 +
 +
padding-top : 0px;
 +
}
 +
 +
.month-picker-fieldset{
 +
padding-top : 0px;
 +
}
  
  
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<body>
 
<body>
<div><h2 style="text-align : center; margin-top: 80px;">Welcome to Protocols</h2></div>
+
<div><h1 style="text-align : center;color:#d80505; margin-top: 80px;">Welcome to Protocols</h1></div>
 +
<hr style="width: 75%; margin : 0 auto;">
  
  
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     <label for="jul" class="month-picker-label" onclick="openPage('w4', this, 'grey')">Degradation </label>
 
     <label for="jul" class="month-picker-label" onclick="openPage('w4', this, 'grey')">Degradation </label>
 
     <input type="radio" name="month" value="aug" id="aug">
 
     <input type="radio" name="month" value="aug" id="aug">
     <label for="aug" class="month-picker-label" onclick="openPage('w5', this, 'grey')">Supernatant<br>experiment</label>
+
     <label for="aug" class="month-picker-label" onclick="openPage('w5', this, 'grey')">Cloning</label>
 
     <input type="radio" name="month" value="sep" id="sep" checked>
 
     <input type="radio" name="month" value="sep" id="sep" checked>
     <label for="sep" class="month-picker-label" onclick="openPage('w6', this, 'grey')">Cloning</label>
+
     <label for="sep" class="month-picker-label" onclick="openPage('w6', this, 'grey')">Parts<br>characterization</label>
    <input type="radio" name="month" value="oct" id="oct" checked>
+
      
    <label for="oct" class="month-picker-label" onclick="openPage('w7', this, 'grey')">Parts<br>characterization</label>
+
      
     <input type="radio" name="month" value="nov" id="nov" checked>
+
     <label for="nov" class="month-picker-label" onclick="openPage('w8', this, 'grey')">Circuit<br> characterization</label>
+
    <input type="radio" name="month" value="dec" id="dec" checked>   
+
    <label for="dec" class="month-picker-label" onclick="openPage('w9', this, 'grey')">Instruments<br>calibration</label>
+
 
+
  
 
</div>
 
</div>
Line 259: Line 293:
 
    
 
    
 
   <div class="centre">
 
   <div class="centre">
<br><br>
+
    <br>
<button class="accordion">Catechu extraction</button>
+
    <button class="accordion">Catechu extraction</button>
<div class="panel" >
+
    <div class="panel" >
  <object class="pdf" data="https://static.igem.org/mediawiki/2018/1/1e/T--Ruia-Mumbai--april_week1.pdf#page=1" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf" data="https://static.igem.org/mediawiki/2018/9/9b/T--Ruia-Mumbai--Catechu-_extraction.pdf#page=1" type="application/pdf"  width="100%" height="100%">
    <param name="view" value="fitH" />
+
          <param name="view" value="fitH" />
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2018/1/1e/T--Ruia-Mumbai--april_week1.pdf">Download PDF</a>.</p>
+
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/9/9b/T--Ruia-Mumbai--Catechu-_extraction.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
<br><br>
+
    <br>
<button class="accordion">Paan color standardization</button>
+
    <button class="accordion">Paan color standardization</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf" data=https://static.igem.org/mediawiki/2018/4/4e/T--Ruia-Mumbai--april_week2.pdf#page=1" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf" data="https://static.igem.org/mediawiki/2018/a/ab/T--Ruia-Mumbai--Paan_color_standardization-.pdf#page=1" type="application/pdf"  width="100%" height="100%">
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2018/4/4e/T--Ruia-Mumbai--april_week2.pdf">Download PDF</a>.</p>
+
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/a/ab/T--Ruia-Mumbai--Paan_color_standardization-.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
<br><br>
+
    <br>
<button class="accordion">Catechu extract amount standardization in media preparation
+
    <button class="accordion">Catechu extract amount standardization in media preparation
 
</button>
 
</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf"  data="https://static.igem.org/mediawiki/2018/4/49/T--Ruia-Mumbai--april_week3.pdf#page=1" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf"  data="https://static.igem.org/mediawiki/2018/2/25/T--Ruia-Mumbai--Catechu_extract_amount_standardization_in_media_preparation.pdf#page=1" type="application/pdf"  width="100%" height="100%">
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2018/4/49/T--Ruia-Mumbai--april_week3.pdf">Download PDF</a>.</p>
+
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/2/25/T--Ruia-Mumbai--Catechu_extract_amount_standardization_in_media_preparation.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
<br><br>
+
<br>
  
 
   </div>
 
   </div>
Line 292: Line 326:
 
<div id="w2" class="tabcontent">
 
<div id="w2" class="tabcontent">
 
   <div class="centre">
 
   <div class="centre">
<br><br>
+
    <br>
<button class="accordion">Media composition</button>
+
    <button class="accordion">Media composition</button>
<div class="panel">
+
    <div class="panel">
  <object  class="pdf" data="https://static.igem.org/mediawiki/2018/1/10/T--Ruia-Mumbai--may_week5.pdf#page=1" type="application/pdf"  width="100%" height="100%">
+
      <object  class="pdf" data="https://static.igem.org/mediawiki/2018/9/98/T--Ruia-Mumbai--Media_composition.pdf
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+
#page=1" type="application/pdf"  width="100%" height="100%">
 +
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/9/98/T--Ruia-Mumbai--Media_composition.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
<br><br>
+
    <br>
<button class="accordion">Effect of autoclaving on CS media</button>
+
    \--<button class="accordion">Effect of autoclaving on CS media</button>
<div class="panel">
+
    <div class="panel">
  <object  class="pdf" data="https://static.igem.org/mediawiki/2018/3/33/T--Ruia-Mumbai--may_week6.pdf#page=1" type="application/pdf"  width="100%" height="100%">
+
      <object  class="pdf" data="https://static.igem.org/mediawiki/2018/1/18/T--Ruia-Mumbai--Effect_of_autoclaving_on_catechu-ilovepdf-compressed.pdf"  width="100%" height="100%">
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2018/3/33/T--Ruia-Mumbai--may_week6.pdf">Download PDF</a>.</p>
+
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/1/18/T--Ruia-Mumbai--Effect_of_autoclaving_on_catechu-ilovepdf-compressed.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>--/
<br><br>
+
    <br>
<button class="accordion">Catechu Tolerance Test</button>
+
    <button class="accordion">Catechu Tolerance Test</button>
<div class="panel">
+
    <div class="panel">
  <object  class="pdf" data="https://static.igem.org/mediawiki/2018/e/eb/T--Ruia-Mumbai--may_week7.pdf#page=1" type="application/pdf"  width="100%" height="100%">
+
      <object  class="pdf" data="https://static.igem.org/mediawiki/2018/a/a0/T--Ruia-Mumbai--Catechu_tolarance_test-.pdf#page=1" type="application/pdf"  width="100%" height="100%">
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="https://static.igem.org/mediawiki/2018/e/eb/T--Ruia-Mumbai--may_week7.pdf">Download PDF</a>.</p>
+
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/a/a0/T--Ruia-Mumbai--Catechu_tolarance_test-.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>    
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 321: Line 356:
 
    
 
    
 
   <div class="centre">
 
   <div class="centre">
<br><br>
+
    <br>
<button class="accordion"> Sampling</button>
+
    <button class="accordion"> Sampling</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf"  data="https://static.igem.org/mediawiki/2018/7/7e/T--Ruia-Mumbai--sampling-.pdf
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
#page=1" type="application/pdf"  width="100%" height="100%">
 +
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/7/7e/T--Ruia-Mumbai--sampling-.pdf
 +
">View PDF</a>.</p>
 
</object>
 
</object>
  
</div>
+
    </div>
<br><br>
+
    <br>
<button class="accordion">Enrichment
+
    <button class="accordion">Enrichment and Isolation
 
</button>
 
</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf"  data="https://static.igem.org/mediawiki/2018/2/28/T--Ruia-Mumbai--Enrichment_and_isolation-.pdf
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
#page=1" type="application/pdf"  width="100%" height="100%">
 +
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/2/28/T--Ruia-Mumbai--Enrichment_and_isolation-.pdf
 +
">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
<br><br>
+
    <br>
<button class="accordion">Isolation</button>
+
       
<div class="panel">
+
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
  <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
</object>
+
</div>
+
<br><br>
+
<button class="accordion">Identification of isolates</button>
+
<div class="panel">
+
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
  <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
</object>
+
</div>
+
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 360: Line 387:
 
    
 
    
 
   <div class="centre">
 
   <div class="centre">
<br><br>
+
    <br>
<button class="accordion">Spectrophotometer</button>
+
    <button class="accordion">Spectrophotometer</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf"  data="https://static.igem.org/mediawiki/2018/7/70/T--Ruia-Mumbai--By_Spectrophotometry.pdf
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
#page=1" type="application/pdf"  width="100%" height="100%">
 +
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/7/70/T--Ruia-Mumbai--By_Spectrophotometry.pdf
 +
">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
<br><br>
+
    <br>
<button class="accordion"> Elisa plate reader</button>
+
    <button class="accordion"> Elisa plate reader</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
      <object class="pdf"  data="https://static.igem.org/mediawiki/2018/d/d4/T--Ruia-Mumbai--By_Elisa_Plate_Reader-.pdf
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
 
 +
#page=1" type="application/pdf"  width="100%" height="100%">
 +
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/d/d4/T--Ruia-Mumbai--By_Elisa_Plate_Reader-.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
  
  
Line 383: Line 414:
 
    
 
    
 
   <div class="centre">
 
   <div class="centre">
<br><br>
+
   
<button class="accordion">Degradation of catechu and tannic acid by supernatant using elisa plate reader and ImageJ </button>
+
<br>
<div class="panel">
+
    <button class="accordion"> pBAD-ompT-CAM-GS-xylE-xylF </button>
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
    <div class="panel">
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
      <object class="pdf"  data="https://static.igem.org/mediawiki/2018/1/12/T--Ruia-Mumbai--pBAD-ompT-CAM-GS-xylE-xylF.pdf#page=1" type="application/pdf"  width="100%" height="100%">
 +
   <p><b>Tap to view it:</b><br>: <a href="https://static.igem.org/mediawiki/2018/1/12/T--Ruia-Mumbai--pBAD-ompT-CAM-GS-xylE-xylF.pdf.pdf">View PDF</a>.</p>
 
</object>
 
</object>
</div>
+
    </div>
 
+
<br>
 
+
<button class="accordion"> pBAD_RBS_ompT_CAM_GS_Tannase_RBS_ompT_galA</button>
 
+
    <div class="panel">
 
+
      <object  class="pdf"  data=" https://static.igem.org/mediawiki/2018/4/4d/T--Ruia-Mumbai--pBAD_RBS_ompT_CAM_GS_Tannase_RBS_ompT_galA.pdf#page=1" type="application/pdf"  width="100%" height="100%">
  </div>
+
   <p><b>Tap to view it:</b><br>: <a href=" https://static.igem.org/mediawiki/2018/4/4d/T--Ruia-Mumbai--pBAD_RBS_ompT_CAM_GS_Tannase_RBS_ompT_galA.pdf.pdf">View PDF</a>.</p>
</div>
+
 
+
<div id="w6" class="tabcontent">
+
 
+
  <div class="centre">
+
 
+
<br><br>
+
<button class="accordion">Digestion </button>
+
<div class="panel">
+
  <object  class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
 
</object>
 
</object>
</div>
+
    </div><br>
<br><br>
+
<button class="accordion"> AraC/pBAD_LambdaCI_TT_pCI_DpnC_TT</button>
<button class="accordion">Ligation</button>
+
    <div class="panel">
<div class="panel">
+
      <object class="pdf"  data=".https://static.igem.org/mediawiki/2018/7/71/T--Ruia-Mumbai--AraC_pC_pBAD_RBS_λCI_TT_pCl-DpnC_TT.pdf#page=1" type="application/pdf"  width="100%" height="100%">
  <object class="pdf"  data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
   <p><b>Tap to view it:</b><br>: <a href=".https://static.igem.org/mediawiki/2018/7/71/T--Ruia-Mumbai--AraC_pC_pBAD_RBS_λCI_TT_pCl-DpnC_TT.pdf.pdf">View PDF</a>.</p>
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
 
</object>
 
</object>
</div>
+
    </div><br>
<br><br>
+
<button class="accordion"> AraC/pBAD_LambdaCI_TT_pCI_GFP</button>
<button class="accordion">Plasmid Prep</button>
+
    <div class="panel">
<div class="panel">
+
      <object class="pdf" data=" https://static.igem.org/mediawiki/2018/8/87/T--Ruia-Mumbai--AraC_pBAD_LambdaCI_TT_pCI_GFP.pdf#page=1" type="application/pdf"  width="100%" height="100%">
  <object class="pdf" data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
   <p><b>Tap to view it:</b><br>: <a href=" https://static.igem.org/mediawiki/2018/8/87/T--Ruia-Mumbai--AraC_pBAD_LambdaCI_TT_pCI_GFP.pdf">View PDF</a>.</p>
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
 
</object>
 
</object>
</div>
+
    </div><br>
<br><br>
+
<button class="accordion"> pBAD_RBS_ompT_XylE</button>
<button class="accordion">Electrophoresis</button>
+
    <div class="panel">
<div class="panel">
+
      <object class="pdf" data=" https://static.igem.org/mediawiki/2018/4/47/T--Ruia-Mumbai--pBAD_RBS_ompT_XylE.pdf#page=1" type="application/pdf"  width="100%" height="100%">
  <object class="pdf" data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
+
   <p><b>Tap to view it:</b><br>: <a href=" https://static.igem.org/mediawiki/2018/4/47/T--Ruia-Mumbai--pBAD_RBS_ompT_XylE.pdf">View PDF</a>.</p>
   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
+
 
</object>
 
</object>
</div>
+
    </div><br>
  </div>
+
</div>
+
  
 +
<button class="accordion">pBAD_RBS_ompT_XylF </button>
 +
    <div class="panel">
 +
      <object  class="pdf"  data="https://static.igem.org/mediawiki/2018/1/12/T--Ruia-Mumbai--pBAD-ompT-CAM-GS-xylE-xylF.pdf
 +
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 +
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<button class="accordion">pBAD_ompT_CAM_GS_Tannase_Biobrick_suffix </button>
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+
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<div id="w7" class="tabcontent">
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    <button class="accordion">pBAD_ompT_galA</button>
 
+
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  <div class="centre">
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<br><br>
+
   <p><b>Tap to view it:</b><br>: <a href=" https://static.igem.org/mediawiki/2018/4/4d/T--Ruia-Mumbai--pBAD_ompT_galA.pdf">View PDF</a></p>
<button class="accordion">XylE </button>
+
<div class="panel">
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</object>
 
</object>
</div>
+
    </div><br>
<br><br>
+
       
<button class="accordion">XylF</button>
+
    <button class="accordion">Plasmid prep by Qiagen</button>
<div class="panel">
+
    <div class="panel">
  <object class="pdf" data="test.pdf#page=2" type="application/pdf"  width="100%" height="100%">
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   <p><b>Example fallback content</b>: This browser does not support PDFs. Please download the PDF to view it: <a href="test.pdf">Download PDF</a>.</p>
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</object>
 
</object>
</div>
+
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<br><br>
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<button class="accordion">Tannase-galA</button>
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<br><br>
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<button class="accordion">Tannase</button>
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<div class="panel">
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<br><br>
+
<button class="accordion">Kill switch(GFP and DpnC)</button>
+
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</div>
 
</div>
  
<div id="w8" class="tabcontent">
+
<div id="w6" class="tabcontent">
 
    
 
    
 
   <div class="centre">
 
   <div class="centre">
<br><br>
+
 
<button class="accordion">w5 Section 1</button>
+
   
<div class="panel">
+
    <br>
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+
    <button class="accordion">2-HMS assay</button>
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 +
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Line 496: Line 502:
  
  
<div id="w9" class="tabcontent">
 
 
 
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<br><br>
 
<button class="accordion">Autoclave</button>
 
<div class="panel">
 
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<br><br>
 
<button class="accordion">UV spectrophotometer</button>
 
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<br><br>
 
<button class="accordion">  Elisa plate reader</button>
 
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$("#apr").click(function() {
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$("#may").click(function() {
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Latest revision as of 09:15, 16 November 2018

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