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| <div class="content_nav_top"> | | <div class="content_nav_top"> |
− | <img src="https://static.igem.org/mediawiki/2018/d/de/T--SJTU-BioX-Shanghai--content_nav_bac_0.png | + | <img src="https://static.igem.org/mediawiki/2018/c/c3/T--SJTU-BioX-Shanghai--content_nav_top.png"/> |
− | "/> | + | |
| <div class="content_nav_logo"> | | <div class="content_nav_logo"> |
| <a href="" title="return to homepage"><img src="https://static.igem.org/mediawiki/2018/1/17/T--SJTU-BioX-Shanghai--content_nav_logo.png | | <a href="" title="return to homepage"><img src="https://static.igem.org/mediawiki/2018/1/17/T--SJTU-BioX-Shanghai--content_nav_logo.png |
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− | Team Work
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− | <img src="https://static.igem.org/mediawiki/2018/2/21/T--SJTU-BioX-Shanghai--content_nav_bac2.png | + | <img class="content_nav_b_bac" src="https://static.igem.org/mediawiki/2018/1/1f/T--SJTU-BioX-Shanghai--content_nav_bottom.png" /> |
− | "/> | + | <a title="Go to story page" href="https://2018.igem.org/Team:SJTU-BioX-Shanghai/Story"><img class="content_nav_b_story_badge" src="https://static.igem.org/mediawiki/2018/d/d0/T--SJTU-BioX-Shanghai--story_badge.png"></a> |
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| <h1>Attributions</h1> | | <h1>Attributions</h1> |
− | <p>We want to thank everyone who helped our idea realized. You gave us a chance to try to conquer the colon cancer, to help others by our own biological way. | + | <p>  We are so grateful for receiving tremendous help from these amazing people on the way turning our idea into reality. You have been not only our helper but also our inspiration and emotional support that motivate us on completing a nearly impossible mission. We couldn’t have accomplished anything without you. We owe you our best thanks. |
| </p> | | </p> |
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− | <h2> | + | <!--********************* storage_box template **************************--> |
− | <a id="section1"><span class="place_holder"></span>Team Work</a> | + | |
− | </h2> | + | <div class="storage_box"> |
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| + | <p>Team Work</p> |
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| <h3>Ideas </h3> | | <h3>Ideas </h3> |
− | <p>We got this project through months of brainstorming by the whole team and this is our third idea. Kairan Zhang first brought up this idea in June after reading a lot of synthetic biology-related articles. And through the whole competition season, he always gave important advice on the project’s design. | + | <p>  Our project came out from months of brainstorming and was first proposed by Kairan Zhang on June with solid foundation laid after great amount of synthetic biology-related readings. Being the core of this project Zhang has been continuously giving new thoughts as well as advice on the project design throughout the season, and has carried out great improvement on our device.</p> |
− | </p> | + | |
| <h3>Biobricks </h3> | | <h3>Biobricks </h3> |
− | <p> Peixiang He, Zhihan Liang, Zhixiang Liu and Yirui Hong were mainly responsible for biobrick-related works in wet lab. Also they prepared engineered bacteria for the cell and mouse experiments. | + | <p>   Peixiang He, Zhihan Liang, Zhixiang Liu and Yirui Hong were mainly responsible for biobrick-related works in wet lab. They were capable of engineering unique bacteria that express exactly how we wanted them to. It is also their efforts on preparing engineered bacteria for further properties examination in cell and mouse experiments.</p> |
− | </p> | + | |
| <h3>Cell Experiments</h3> | | <h3>Cell Experiments</h3> |
− | <p>Cancer cell lines HT29 and normal epithelial cell lines HACAT were maintained by Yuncong Ran, Shiyu Sun, Ruoyu Xia and Shihao Bai. They also did the short peptides binding experiments to test if the peptides can bind to T antigens on cancer cells. | + | <p>  Cancer cell lines HT29 and normal epithelial cell lines HACAT were cultured by Yuncong Ran, Shiyu Sun, Ruoyu Xia and Shihao Bai to serve as the subjects for multiple short peptides adhesive property examinations. The examinations aimed to testify the specificity of antigen-antibody binding between our bacteria and different cell lines. |
| </p> | | </p> |
− | <h3>Ultrasound imaging</h3> | + | <h3>Mouse Experiments</h3> |
− | <p>Ultrasound-related experiments were mainly designed and performed by Qingwei. He also developed the Matlab code for ultrasound image porcessing job. Shiyu Sun helped to do this job when Qingwei was busy with the team wiki. | + | <p>  Mouse experiments are among the most important parts of our projects and they are mainly designed and performed by Shuyu Zheng and CHIA-YI HSIAO, including diseased mouse model induction as well as device testifying. |
| </p> | | </p> |
| + | <h3>Ultrasound imaging</h3> |
| + | <p>  Ultrasound-related experiments were mainly designed and performed by Qingwei Fang. He also developed the Matlab code for ultrasound image processing job. And Shiyu Sun carried on doing this job when Qingwei was overwhelmed with the team wiki.</p> |
| <h3>MRI imaging</h3> | | <h3>MRI imaging</h3> |
− | <p>Qingwei Fang recorded MRI imaging experiment and assisted in experiment design and data processing. Kuan Yao helped record the experiments when Qingwei was busy with wiki developement. | + | <p>  Qingwei Fang recorded MRI imaging experiment and assisted in experiment design as well as data processing. And Kuan Yao helped with the record of the experiments while Qingwei being busy on wiki development.</p> |
− | </p> | + | |
| <h3>Modeling</h3> | | <h3>Modeling</h3> |
− | <p>Shuyu Zheng, Shiyu Sun, Xin Zhang, Anyuan Tian and Yulin Liao did all the modeling work for our projects. | + | <p>  Shuyu Zheng, Shiyu Sun, Xin Zhang, Anyuan Tian and Yulin Liao did all the modeling work for our projects. |
| </p> | | </p> |
| <h3>Human Practices</h3> | | <h3>Human Practices</h3> |
− | <p>Bozitao Zhong, Weiwen Wu, Kuan Yao and Shihao Bai were in charge of the human practices part. Bozitao Zhong was the leader of this part. Weiwen Wu brought up a lot of new ideas. Kuan Yao organized the exhibition and Shihao Bai helped the research in Canada. | + | <p>  Bozitao Zhong, Weiwen Wu, Kuan Yao and Shihao Bai were in charge of the human practices part. Bozitao Zhong was the leader of this part. Weiwen Wu brought up a lot of new ideas. Kuan Yao organized the exhibition and Shihao Bai helped with the research in Canada. |
| </p> | | </p> |
| <h3>Art</h3> | | <h3>Art</h3> |
− | <p>Our team’s logo, banner, posters, uniforms and the layout of our wiki were all designed by Weiwen Wu. She helped us to rank the second best poster in the fifth CCiC. Yuncong Ran also helped to design the posters exhibited in our 10th anniversary exhibition. | + | <p>  Our team’s logo, banner, posters, uniforms and the layout of our wiki were all designed by Weiwen Wu. She helped us to rank the second best poster in the fifth CCiC. Yuncong Ran also helped to design the posters exhibited in our 10th anniversary exhibition. |
| </p> | | </p> |
| <h3>Wiki</h3> | | <h3>Wiki</h3> |
− | <p>Our wiki were developed by all of us but Qingwei Fang and Yulin Liao construct the website’s frame for us in advance. Qingwei were in charge with communicating with the designer Weiwen and the development of wiki on computers while Yulin worked on the mobile web. | + | <p>  Our wiki were developed by all of us but Qingwei Fang and Yulin Liao construct the website’s frame for us in advance. Qingwei were in charge with communicating with the designer Weiwen and the development of wiki on computers while Yulin worked on the mobile web. |
| </p> | | </p> |
| <h3>Presentation</h3> | | <h3>Presentation</h3> |
− | <p>Jiayi Xiao is our presentation mastermind. She attended the Asia-Pacific iGEM Conference on behalf of our team. Later, she instructed the presentation on the fifth CCiC. Our last presentation on Giant Jamboree was also conducted by her. Her speech is really appealing and full of passion. | + | <p>  Hsiao Chia Yi is our presentation mastermind. She attended the Asia-Pacific iGEM Conference on behalf of our team. Later, she instructed the presentation on the fifth CCiC. Our last presentation on Giant Jamboree was also conducted by her. Her speech is really appealing and full of passion. |
| </p> | | </p> |
| | | |
− | <h2> | + | </div> |
− | <a id="section2"><span class="place_holder"></span>Advisor Help</a> | + | |
− | </h2> | + | |
| + | </li> |
| + | <li class="storage_box_ele"> |
| + | <div class="storage_header"> |
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| + | <p>Advisor Support</p> |
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| <h3>Zhijun Wang</h3> | | <h3>Zhijun Wang</h3> |
− | <p>Zhijun Wang was a member of the 2017 iGEM team. He designed the mechanism of recruitment after last year's competition and then participated in the selection and training of new team members. After that, he helped the preparation of iGEM 2018 and clarified some confusion related to ultrasound and modeling. He also gave advice on the construction of our Wiki. | + | <p>  Zhijun Wang helped us with recruitment of iGEM 2018 team and clarified some confusion related to ultrasound and modeling. He has also given advice on the determination of project and construction of our Wiki. |
| </p> | | </p> |
| <h3>Zhuoyang Chen</h3> | | <h3>Zhuoyang Chen</h3> |
− | <p>He was the advisor of our team. He helped molecular operations and troubleshoot in the wet lab. Furthermore, he helped the team work, keeping everything in progress and provided information about the competition. | + | <p>  Chen has helped a lot on troubleshooting in wet lab. And as a senior, he guided our team work, kept everything in progress and provided information about the competition. </p> |
− | </p> | + | |
| <h3>Chang Gao</h3> | | <h3>Chang Gao</h3> |
− | <p>Chang Gao is an advisor in our team. She was an active team member last year, so her experiences were used to determine project, design and arrange experiments and so on. She gave many protocols of experiments as well, such as constructing plasmids, western blotting. Besides, she helped finish the western blotting. | + | <p>  Chang Gao was an active team member last year, so her experiences were extremely useful to determine our project, experiment design, arranging experiments and so on. She has shared many protocols of experiments as well, including constructing plasmids and western blotting. She was also a great help on finishing western blotting. |
| + | </p> |
| + | <h3>Xiang Chen</h3> |
| + | <p>  Xiang Chen provided us the lab and equipment to do the micro-fluidic experiment.. |
| </p> | | </p> |
| + | </div> |
| + | </li> |
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− | <h2> | + | <p>Supervisor Support</p> |
− | <a id="section3"><span class="place_holder"></span>Supervisor Support</a> | + | <img /> |
− | </h2> | + | |
| + | </div> |
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| <h3>Lin He</h3> | | <h3>Lin He</h3> |
− | <p>Lin He is the supervisor of our team and the director of Bio-X, who gave us funding and university-related support. He founded iGEM team SJTU-BioX-Shagnhai ten years ago and has been supporting us since then. | + | <p>  Lin He is the supervisor of our team and the director of Bio-X, who gave us funding and university-related support. He founded iGEM team SJTU-BioX-Shanghai ten years ago and has been supporting us since then. |
| </p> | | </p> |
| <h3>Gang Ma</h3> | | <h3>Gang Ma</h3> |
− | <p>Gang Ma is the supervisor of our team and the professor of Bio-X, who gave us advice and instructions. He cheerer us up when we felt frustrated. He encouraged us to overcome our limitations and keep trying for the best. | + | <p>  Gang Ma is the supervisor of our team and the professor of Bio-X, who gave us advice and instructions. He cheerer us up when we felt frustrated. He encouraged us to overcome our limitations and keep trying for the best. |
| </p> | | </p> |
| <h3>Yushu Wang</h3> | | <h3>Yushu Wang</h3> |
− | <p>Yushu Wang spent a lot of time and energy on us, helping with all kinds of problems, from experiments materials supply to troubleshooting. She is just like a sister to all of us, so warm and kind. | + | <p>  Yushu Wang spent a lot of time and energy on us, helping with all kinds of problems, from experiments materials supply to troubleshooting. She is just like a sister to all of us, so warm and kind. |
− | </p>
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− | <h3>Xia Zhang</h3>
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− | <p>Xia Zhang is a teacher in SJTU Experimental Teaching Center. She taught us a lot about molecular level experiments and provided the EGFP plasmid that was used in antibody binding experiment.
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| </p> | | </p> |
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− | <h2> | + | <p>General Support</p> |
− | <a id="section4"><span class="place_holder"></span>General Support</a> | + | <img /> |
− | </h2> | + | |
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| <h3>Research institute of Bio-X</h3> | | <h3>Research institute of Bio-X</h3> |
− | <p>During the whole preparation procedure of the iGEM competition, Bio-X offered us a great support in all aspects. Thanks to their providing of experimental funds and drugs. | + | <p>  During the whole preparation procedure of the iGEM competition, Bio-X offered us a great support in all aspects. Thanks to their providing of experimental funds and drugs. |
| </p> | | </p> |
| <h3>Shanghai Jiao Tong University</h3> | | <h3>Shanghai Jiao Tong University</h3> |
− | <p>Our university gives us logistics support for the whole project. Thanks for the substantial support given by our university. | + | <p>  Our university gives us logistics support for the whole project. Thanks for the substantial support given by our university. |
| </p> | | </p> |
| <h3>School of Life Sciences and Biotechnology, SJTU</h3> | | <h3>School of Life Sciences and Biotechnology, SJTU</h3> |
− | <p>During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. | + | <p>  During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. |
| </p> | | </p> |
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| + | <p>Technique Support</p> |
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− | <h2>
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− | <a id="section5"><span class="place_holder"></span>Technique support</a>
| + | <h3>MRI imaging--Prof. Yiping Du</h3> |
− | </h2>
| + | <p>  The MRI experiment was directed by Professor Yiping Du, Dean of the IMIT (Institute of Medical Imaging Technology), Shanghai Jiaotong University. Zhang Yufei designed, conducted experiments and completed data processing. |
− | <h3>MRI imaging</h3> | + | |
− | <p>The MRI experiment was directed by Professor Yiping Du, Dean of the IMIT (Institute of Medical Imaging Technology), Shanghai Jiaotong University. Zhang Yufei designed, conducted experiments and completed data processing. | + | |
| </p> | | </p> |
| + | <h3>CRC induction--Qian Yu</h3> |
| + | <p>  Yu is an doctoral student who focus on studies related to colorectal cancer mouse model. He is our advicer for the CRC induction process as well as other related experiments. To be precise he offers help on CRC induction, sectioning, as well as cytometry. |
| + | </p> |
| + | <h3>Clinical ultrasonic equipment--Doctor Tian Tsai</h3> |
| + | <p>  Doctor Tsai is a veterinarian in HongBiZi pet clinic. He has offered us clinical ultrasound equipment free of charge and has given us very useful clinical advice on examining real animals in vitro. </p> |
| + | |
| + | <h3>Overall support--Qingchun Yang</h3> |
| + | <p>  Yang is a doctoral student in our PI’s group who has long been very kind and helpful on our project. As a senior, she helped us with the orientation in the lab while she also offers tremendous help and advice on all molecular, cell and mouse experiments, including immunohistochemistry, paraffin sectioning, and bacteria transduction. She enlightens us on trying new procedures to better demonstrate our examination and has long been our inspiration and support. </p> |
| + | |
| + | <h3>Micro-fluidic experiment instruction – Chao Han</h3> |
| + | <p>  Chao Han is a doctoral student of Shanghai General Hospital. He helped with the micro-fluidic chip manufacturing and gave us instructions on chip preparation, pump operation, and sample loading. He also helped with the observation of chips. </p> |
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| + | <p>Materials Support</p> |
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| + | <h3>Xia Zhang</h3> |
| + | <p>  Xia Zhang is a teacher in SJTU Experimental Teaching Center. She provided us with plasmid containing egfp gene which was used in the adhesion experiments. |
| + | </p> |
| + | <h3>Gengming Niu</h3> |
| + | <p>  Gengming Niu, from the Fifth People’s Hospital of Shanghai, Fudan University provided the human cell lines HT29 to us. We used it to test our system in vitro. |
| + | <h3>Guoqiang Chen</h3> |
| + | <p>  Guoqiang Chen is a professor in Tsinghua University. He provided us with plasmid containing the acoustic reporter gene which was used in the ultrasound reporting experiment. |
| + | <h3>2018 iGEM teams</h3> |
| + | <p>  We also got materials support from 2018 iGEM teams, further details can be found at<a title="collaborations" href="https://2018.igem.org/Team:SJTU-BioX-Shanghai/Collaborations">Collabrations</a> page. |
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| <div style="display: flex;flex-direction: row;justify-content: center;align-items: center;width:100%;height: 100%;"> | | <div style="display: flex;flex-direction: row;justify-content: center;align-items: center;width:100%;height: 100%;"> |
− | <div style="width: auto;height: auto;"> | + | |
| <div class="zoom_out_album"> | | <div class="zoom_out_album"> |
| <img id="zoom_out_img"/> | | <img id="zoom_out_img"/> |
| <p class="fig_illustration"></p> | | <p class="fig_illustration"></p> |
− | <img class="close_icon"/> | + | |
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− | <!--***************************This is the end of the content***********************************--> | + | <!--***************************This is the end of the content***********************************--> |
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| <div id = "footer"> | | <div id = "footer"> |