Difference between revisions of "Team:NCTU Formosa/Collaborations"
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<img src="https://static.igem.org/mediawiki/2018/5/57/T--NCTU_Formosa--collaboration_title.png" class="title_title"> | <img src="https://static.igem.org/mediawiki/2018/5/57/T--NCTU_Formosa--collaboration_title.png" class="title_title"> | ||
<div class="edu1"> | <div class="edu1"> | ||
| − | <div class="block title_1"><p>Team: <a href="" class="team">NTHU_Formosa</a></p></div> | + | <div class="block title_1"><p>Team: <a href="https://2018.igem.org/Team:NTHU_Formosa/Collaborations" class="team">NTHU_Formosa</a></p></div> |
<div class="block title_2"><p>Troubleshooting -- Wiki course</p></div> | <div class="block title_2"><p>Troubleshooting -- Wiki course</p></div> | ||
| − | <div class="block content_1"><p> On August 23<sup>th</sup>, we prepared a wiki course for NTHU_Formosa, helping them to troubleshoot on their difficulties of writing their wiki. First of all, we taught them the concept and process of designing a website as an introduction. Second, we introduced how to use HTML, CSS, as well as JavaScript. Third, we helped them to build up a simple RWD website step by step to make them more familiar with the web languages. Finally, we gave them some tips and advices on editing their wiki. We’re really happy about having such a chance to share our knowledge with other iGEM teams! Hope the course could help them in a meaningful way. We’re looking forward to have further collaboration with NTHU_Formosa.</p></div> | + | <div class="block content_1"> |
| + | <p> | ||
| + | On August 23<sup>th</sup>, we prepared a wiki course for NTHU_Formosa, helping them to troubleshoot on their difficulties of writing their wiki. First of all, we taught them the concept and process of designing a website as an introduction. Second, we introduced how to use HTML, CSS, as well as JavaScript. Third, we helped them to build up a simple RWD website step by step to make them more familiar with the web languages. Finally, we gave them some tips and advices on editing their wiki. We’re really happy about having such a chance to share our knowledge with other iGEM teams! Hope the course could help them in a meaningful way. We’re looking forward to have further collaboration with NTHU_Formosa. | ||
| + | </p> | ||
| + | </div> | ||
<img src="https://static.igem.org/mediawiki/2018/9/9f/T--NCTU_Formosa--NTHU_3.png" class="photo_1"> | <img src="https://static.igem.org/mediawiki/2018/9/9f/T--NCTU_Formosa--NTHU_3.png" class="photo_1"> | ||
<div class="explanation"> | <div class="explanation"> | ||
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</div> | </div> | ||
<img src="https://static.igem.org/mediawiki/2018/e/e1/T--NCTU_Formosa--NTHU_1.png" class="photo_1"> | <img src="https://static.igem.org/mediawiki/2018/e/e1/T--NCTU_Formosa--NTHU_1.png" class="photo_1"> | ||
| − | <div class="explanation" | + | <div class="explanation" style="margin-bottom: 7vw;"> |
<svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | ||
Figure 2: NCTU_Formosa and NTHU_Formosa team members | Figure 2: NCTU_Formosa and NTHU_Formosa team members | ||
| + | </div> | ||
| + | |||
| + | <div class="block title_2"><p>Characterization of Parts from NTHU_Formosa</p></div> | ||
| + | <div class="block content_1"> | ||
| + | <p> | ||
| + | We characterize the part of NTHU_Formosa team. These inserted vectors, including <a href="http://parts.igem.org/Part:BBa_K2635001">pCAG-GBP</a>, pTRE3G-LuxAB, pCMV-LuxCDEfrp, <a href="http://parts.igem.org/Part:BBa_K2635000">pTRE3G-mCherry</a>, are to produce receptor, enzyme, substrate, which are essential in the process of emitting the light. We helped with NTHU_Formosa to check if these vectors are correct. The results in the figure below shows these vectors are connected correctly. | ||
| + | </p> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/0/01/T--NCTU_Formosa--NTHU_Collaboration_Gel.png" class="photo_2"> | ||
| + | <div class="explanation"> | ||
| + | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | ||
| + | Figure 3: The result of DNA electrophoresis.(sample name: pCAG-GBP, pTRE3G-LuxAB, pCMV-LuxCDEfrp, pTRE3G-mCherry) | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/7/7c/T--NCTU_Formosa--collaboration4.jpg" class="photo_1"> | ||
| + | <div class="explanation"> | ||
| + | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | ||
| + | Figure 4: We loaded the sample into gel. | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/c/c1/T--NCTU_Formosa--collaboration3.jpg" class="photo_1"> | ||
| + | <div class="explanation" style="margin-bottom: 7vw;"> | ||
| + | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | ||
| + | Figure 5: We read the result of DNA electrophoresis together. | ||
</div> | </div> | ||
<div class="block title_2"><p>Bidirectional collaboration with NTHU_Formosa</p></div> | <div class="block title_2"><p>Bidirectional collaboration with NTHU_Formosa</p></div> | ||
| − | <div class="block content_1"><p> | + | <div class="block content_1"> |
| − | <img src="" class="photo_1"> | + | <p> |
| + | After inducing our <i>E. coli</i>, we wanted double check that our constructs were correctly expressed. To do this, we invited members of team NTHU_Formosa to come to our lab on October 6<sup>th</sup>, where they spent an afternoon running an SDS-PAGE of Enterocin B and Enterocin 96. The results are shown in the figures below. Thanks to NTHU_Formosa’s help, we confirmed that our peptides were being expressed correctly. | ||
| + | </p> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/0/02/T--NCTU_Formosa--collaboration2.jpg" class="photo_1"> | ||
<div class="explanation"> | <div class="explanation"> | ||
<svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | ||
| − | Figure | + | Figure 6: We taught NTHU_Formosa how to prepare the gel. |
</div> | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/6/66/T--NCTU_Formosa--collaboration1.jpg" class="photo_1"> | ||
| + | <div class="explanation" style="margin-bottom: 7vw;"> | ||
| + | <svg class="icon" aria-hidden="true" data-prefix="fas" data-icon="arrow-circle-up" class="svg-inline--fa fa-arrow-circle-up fa-w-16" role="img" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path fill="currentColor" d="M8 256C8 119 119 8 256 8s248 111 248 248-111 248-248 248S8 393 8 256zm143.6 28.9l72.4-75.5V392c0 13.3 10.7 24 24 24h16c13.3 0 24-10.7 24-24V209.4l72.4 75.5c9.3 9.7 24.8 9.9 34.3.4l10.9-11c9.4-9.4 9.4-24.6 0-33.9L273 107.7c-9.4-9.4-24.6-9.4-33.9 0L106.3 240.4c-9.4 9.4-9.4 24.6 0 33.9l10.9 11c9.6 9.5 25.1 9.3 34.4-.4z"></path></svg> | ||
| + | Figure 7: NTHU_Formosa loaded sample (Enterocin B and Enterocin 96) into gel. | ||
| + | </div> | ||
| + | |||
| + | <div class="title_3"><p>To learn more about their perspective, check out their <a href="https://2018.igem.org/Team:NTHU_Formosa/Collaborations">wiki</a>.</p></div> | ||
| − | |||
</div> | </div> | ||
Latest revision as of 16:09, 7 December 2018
Team: NTHU_Formosa
Troubleshooting -- Wiki course
On August 23th, we prepared a wiki course for NTHU_Formosa, helping them to troubleshoot on their difficulties of writing their wiki. First of all, we taught them the concept and process of designing a website as an introduction. Second, we introduced how to use HTML, CSS, as well as JavaScript. Third, we helped them to build up a simple RWD website step by step to make them more familiar with the web languages. Finally, we gave them some tips and advices on editing their wiki. We’re really happy about having such a chance to share our knowledge with other iGEM teams! Hope the course could help them in a meaningful way. We’re looking forward to have further collaboration with NTHU_Formosa.
Figure 1: The wiki course (when talking about CSS)
Figure 2: NCTU_Formosa and NTHU_Formosa team members
Characterization of Parts from NTHU_Formosa
We characterize the part of NTHU_Formosa team. These inserted vectors, including pCAG-GBP, pTRE3G-LuxAB, pCMV-LuxCDEfrp, pTRE3G-mCherry, are to produce receptor, enzyme, substrate, which are essential in the process of emitting the light. We helped with NTHU_Formosa to check if these vectors are correct. The results in the figure below shows these vectors are connected correctly.
Figure 3: The result of DNA electrophoresis.(sample name: pCAG-GBP, pTRE3G-LuxAB, pCMV-LuxCDEfrp, pTRE3G-mCherry)
Figure 4: We loaded the sample into gel.
Figure 5: We read the result of DNA electrophoresis together.
Bidirectional collaboration with NTHU_Formosa
After inducing our E. coli, we wanted double check that our constructs were correctly expressed. To do this, we invited members of team NTHU_Formosa to come to our lab on October 6th, where they spent an afternoon running an SDS-PAGE of Enterocin B and Enterocin 96. The results are shown in the figures below. Thanks to NTHU_Formosa’s help, we confirmed that our peptides were being expressed correctly.
Figure 6: We taught NTHU_Formosa how to prepare the gel.
Figure 7: NTHU_Formosa loaded sample (Enterocin B and Enterocin 96) into gel.
To learn more about their perspective, check out their wiki.
