Difference between revisions of "Team:Ruia-Mumbai/Basic Part"

 
(39 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
{{Ruia-Mumbai}}
 
{{Ruia-Mumbai}}
 
<html>
 
<html>
 +
<style type="text/css">
 +
  .image1, .image2{
  
−
Basic Parts:
+
    height: 15rem;
 +
    width: auto;
 +
    display: block;
 +
    margin : 0 auto;
 +
  }
  
−
BBa_K2749017
+
.image3{
−
xylE
+
−
The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde. It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest.
+
−
Structure:
+
−
<img src="">
+
−
Fig1: 3D-Structure of enzyme catechol-2,3-dioxygenase (xylE)
+
−
<img src="">
+
−
Reaction Image taken from paper:
+
−
https://www.ingentaconnect.com/contentone/scs/chimia/2017/00000071/00000010/art00015?crawler=true&mimetype=application/pdf
+
  
 +
    height: 10rem;
 +
    width: auto;
 +
    display: block;
 +
    margin : 0 auto;
 +
}
  
 +
@media only screen and (max-width: 767px)
 +
{
 +
  .image1{
 +
    height: 10rem;
  
 +
  }
  
 +
  .image2{
 +
    height: 7rem;
  
 +
  }
  
 +
  .image3{
 +
    height: 6rem;
 +
  }
 +
 +
 +
 +
}
 +
</style>
 +
 +
 +
 +
 +
 +
 +
 +
<body>
 +
    <div class="container">
 +
        <div class="row">
 +
            &nbsp;
 +
        </div>
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
<br>
 +
<br>
 +
 +
<h1 style="text-align:center; color:#d80505 ;">BASIC PART</h1><hr>
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
        <div class="row">
 +
<strong>Basic Parts:<a href="http://parts.igem.org/Part:BBa_K2749017">BBa_K2749017</a></strong>
 +
</div>
 +
        <div class="row">
 +
The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde. <br>
 +
It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest.
 +
</div>
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
        <div class="row">
 +
<img src="https://static.igem.org/mediawiki/2018/6/6c/T--Ruia-Mumbai--composite_part.png" class="image3"><br>
 +
</div>
 +
        <div class="row">
 +
<a href="https://www.ingentaconnect.com/contentone/scs/chimia/2017/00000071/00000010/art00015?crawler=true&mimetype=application/pdf">Reaction Image Source</a>
 +
</div>
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
       
 +
<div class="row">
 +
<strong>3D Structure of enzyme catechol-2,3-dioxygenase</strong>
 +
</div>
 +
<hr>
 +
 +
<div class="row">
 +
<img src="https://static.igem.org/mediawiki/2018/0/09/T--Ruia-Mumbai--XylE3D.png" class="image1">
 +
</div>
 +
       
 +
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
       
 +
 +
<div class="row">
 +
 +
<strong> Construct of the our enzyme module</strong>
 +
</div><hr>
 +
<div class="row">
 +
<img src="https://static.igem.org/mediawiki/2018/3/34/T--Ruia-Mumbai--xylE_character.png" class="image2" ><br>
 +
</div>
 +
 +
       
 +
<div class="row">
 +
            &nbsp;
 +
        </div>
 +
       
 +
 +
 +
    </div>   
 +
</body>
 
</html>
 
</html>
 +
{{Ruia-Mumbai/Footer}}

Latest revision as of 17:03, 7 December 2018

 
 


BASIC PART

 
Basic Parts:BBa_K2749017
The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde.
It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest.
 
 

Reaction Image Source
 
3D Structure of enzyme catechol-2,3-dioxygenase
 
Construct of the our enzyme module

 

Our co-ordinates:

L .Nappo Road, Matunga
Dadar East,Mumbai,
Maharashtra 400019,
India

catechewingcoli@gmail.com

FOLLOW US ON: