Difference between revisions of "Team:NCKU Tainan"

Of CO₂urse

A biological approach to “off” CO₂ emission

By 2018 iGEM NCKU_TAINAN

Ever since the 90s when concern over the impact of carbon emission on our environment was first raised, global-wide efforts in reducing emission have been met with mixed results. Just 2017 alone the global emission level grew by 1.4%. This year, iGEM NCKU_Tainan will design a device capable of piping CO₂ and convert such carbon source into biomass via intergrating a non-native Calvin-Benson-Bassham cycle into E. coli. “Of Course” is a biological approach to “off” CO₂ emission through the RuBisCO and PRK genes from Synechococcus sp, which encode for major enzymes involved in carbon fixation. Industrial gases will enter a pipe (inlet) at the bottom of a cylindrical container, flow through a ceramic nozzle and mix with liquids containing engineered E. coli that consumes CO₂. Furthermore, CO₂ concentration will be determined by monitoring the corresponding change in pH using an asr (acid shock RNA) promoter containing E. coli with a sfGFP as reporter. Our ultimate goal is that this novel approach will reduce CO₂ in order to slow down global climate changes.