Difference between revisions of "Team:ICT-Mumbai/InterLab"
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This year's InterLab study was conducted to answer the following question: | This year's InterLab study was conducted to answer the following question: | ||
<h5> Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD? </h5> | <h5> Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD? </h5> | ||
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Team ICT-Mumbai participated in the interlab study this year. The plate reader protocol of the plate reader study as given on the measurements page of the iGEM website was strictly followed (the protocol can be downloaded here) and resulting data added to the provided Excel sheet which was subsequently uploaded (the Excel sheet can be downloaded here). | Team ICT-Mumbai participated in the interlab study this year. The plate reader protocol of the plate reader study as given on the measurements page of the iGEM website was strictly followed (the protocol can be downloaded here) and resulting data added to the provided Excel sheet which was subsequently uploaded (the Excel sheet can be downloaded here). | ||
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The figures given below show the obtained results for the cell measurement protocol of this year’s interlab study. | The figures given below show the obtained results for the cell measurement protocol of this year’s interlab study. | ||
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<img src="https://static.igem.org/mediawiki/2018/c/cc/T--ICT-Mumbai--Interlab_Abs_0.png"></img> | <img src="https://static.igem.org/mediawiki/2018/c/cc/T--ICT-Mumbai--Interlab_Abs_0.png"></img> | ||
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<img src="https://static.igem.org/mediawiki/2018/e/e7/T--ICT-Mumbai--Interlab_Abs_6.png"></img> | <img src="https://static.igem.org/mediawiki/2018/e/e7/T--ICT-Mumbai--Interlab_Abs_6.png"></img> | ||
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<img src="https://static.igem.org/mediawiki/2018/c/ce/T--ICT-Mumbai--Interlab_Floursc_0.png"></img> | <img src="https://static.igem.org/mediawiki/2018/c/ce/T--ICT-Mumbai--Interlab_Floursc_0.png"></img> | ||
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<img src="https://static.igem.org/mediawiki/2018/b/b1/T--ICT-Mumbai--Interlab_Floursc_6.png"></img> | <img src="https://static.igem.org/mediawiki/2018/b/b1/T--ICT-Mumbai--Interlab_Floursc_6.png"></img> | ||
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Revision as of 07:10, 6 August 2018
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InterLab Study
This year's InterLab study was conducted to answer the following question:
Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?
Team ICT-Mumbai participated in the interlab study this year. The plate reader protocol of the plate reader study as given on the measurements page of the iGEM website was strictly followed (the protocol can be downloaded here) and resulting data added to the provided Excel sheet which was subsequently uploaded (the Excel sheet can be downloaded here).
The figures given below show the obtained results for the cell measurement protocol of this year’s interlab study.
Download InterLab Study Excel sheet