Difference between revisions of "Team:NCKU Tainan/Hardware"

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               <h1 class="head2">Biosafety</h1>
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               <h1 class="head2">Hardware</h1>
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                 <p class="pcontent">NCKU-iGEM team is concerned with biosafety and biosecurity in every aspect of our project. We realize one major responsibility of iGEMers is to show the public that the project as well as synthetic will not do harm to the environment and the society. We make checks on every safety detail including materials, lab, designed device, and every team member.</p>              
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                <h3>Accomplishment</h3>
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                1. Built and characterized a functional prototype for carbon utilization system in industry sector.</br>
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                2. Created an instructional video, a manual and lists of materials.</br>
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                3. Implemented Bio-safety to our device.</br>
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                4. Integrated with modelling.</br>
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                 <h3>1. Material and project safety? OF COURSE</h3>
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                 <h3>Introduction</h3>
                 <div id="pt"><h8>Chassis organism</h8></br>
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                 <div id="pt"><p class="pcontent">Nearly 40% of CO2 emissions are attributable to industries. The goal of our project is to solve the CO2 problem by using engineered E.coli to fix carbon dioxide emitted from industries and convert it into bio-product, pyruvate. To accomplish our goal, we designed a device that will upscale our project to be used on field and we aim to integrate the device into industrial IGCC system. </p></div>
                <p class="pcontent">We chose E. coli as our chassis organism. Three lab strains were selected: “DH5 alpha”, “BL21(DE3)”, “W3110”, and ”W3110 (L5T7)”. These lab strains are reported to be not pathogenic to human. We construct our design with these strains in authorized lab (biosafety level 1 lab) only.</p>
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                <div id="pt"><h8>New parts construction</h8></br>
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                <p class="pcontent">We chose E. coli as our chassis organism. Three lab strains were selected: “DH5 alpha”, “BL21(DE3)”, “W3110”, and ”W3110 (L5T7)”. These lab strains are reported to be not pathogenic to human. We construct our design with these strains in authorized lab (biosafety level 1 lab) only.</p>
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                <h3>2. Establishment of the iGEM lab</h3>
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                <div id="pt"><p class="pcontent">This is the third year since the establishment of iGEM NCKU-Tainan team. We have raised the awareness of synthetic biology in our campus. Mrs. Jenny Su and the College of Engineering decided to give us a headquarter which contains a meeting room and a lab. To meet the lab standard of CDC (Center for Disease Control), we made contact with Mrs. Liu Yee Fen, the contact person of Center for Occupational safety and Health and Environmental Protection, which is the authorized unit in our campus responsible for lab safety and security. With the assistance of our instructor Dr. Ng, we successfully certify our lab to BSL1 saftey level one lab. Mrs. Liu gave us some lab security instructions and advice. She also suggested that each of our members should attend the biosafety lab training program.
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                 <div id="pt"><p class="pcontent">Expect the lab in iGEM headquarter, wet team has constructed out design in various labs whose supervisors are Dr. I-Son Ng, Dr. I-Hsiu Huang, Dr. Han-Ching Wang, and Dr. Hashimoto Masayuki. The labs mentioned above are all certified biosafety level one.</p></div>
 
                 <div id="pt"><p class="pcontent">Expect the lab in iGEM headquarter, wet team has constructed out design in various labs whose supervisors are Dr. I-Son Ng, Dr. I-Hsiu Huang, Dr. Han-Ching Wang, and Dr. Hashimoto Masayuki. The labs mentioned above are all certified biosafety level one.</p></div>
  
 
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                 <h3>3. Safety training for every teammate</h3>
 
                 <h3>3. Safety training for every teammate</h3>
 
                 <div id="pt"><p class="pcontent">It is a must for anyone to pass the authorized training section before conducting any experiments in lab. Since our applied design must be tested with constructed bacteria, each of our team members will have chances to approach the lab. We decided that whole team should be certified to do the experiments in biosafety level one lab. Team members from biotechnology have already passed the training session, and the rest of team members have passed the training online course. We believe that learning safety and security knowledge lower the risk when we conducted experiments inside biolab.</p></div>
 
                 <div id="pt"><p class="pcontent">It is a must for anyone to pass the authorized training section before conducting any experiments in lab. Since our applied design must be tested with constructed bacteria, each of our team members will have chances to approach the lab. We decided that whole team should be certified to do the experiments in biosafety level one lab. Team members from biotechnology have already passed the training session, and the rest of team members have passed the training online course. We believe that learning safety and security knowledge lower the risk when we conducted experiments inside biolab.</p></div>

Revision as of 13:37, 14 September 2018

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