Difference between revisions of "Team:NCKU Tainan/Protocol"
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<div id="list-item-2"> | <div id="list-item-2"> | ||
<h3>Arduino</h3> | <h3>Arduino</h3> | ||
| − | < | + | <div> |
| − | + | <div class="row"> | |
| − | + | <a class="btn col-md-12" data-toggle="collapse" href="#test" role="button" aria-expanded="false" | |
| − | + | aria-controls="multiCollapseExample1"> | |
| − | + | test | |
| − | </ | + | <i class="fa fa-arrow-down fa-10" aria-hidden="true"></i> |
| + | </a> | ||
| + | </div> | ||
| − | < | + | <div class="collapse multi-collapse" id="test""> |
| − | < | + | <table class="card card-body"> |
| − | + | <tr> | |
| − | + | <th></th> | |
| − | + | <th>20 μl system</th> | |
| + | <th>50 μl system</th> | ||
| + | </tr> | ||
| − | + | <tr> | |
| − | + | <td>Template</td> | |
| − | + | <td>12～20 ng</td> | |
| − | + | <td>30～50 ng</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>Forward primer</td> | |
| − | + | <td>1.0 μl</td> | |
| − | + | <td>2.5 μl</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>Reverse primer</td> | |
| − | + | <td>1.0 μl</td> | |
| − | + | <td>2.5 μl</td> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <td>dNTP</td> | |
| − | <td> | + | <td>1.6 μl</td> |
| − | + | <td>4.0 μl</td> | |
| − | + | </tr> | |
| − | + | ||
| + | <tr> | ||
| + | <td>10x Buffer</td> | ||
| + | <td>2.0 μl</td> | ||
| + | <td>5.0 μl</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <table class="card card-body"> | ||
| + | <tr> | ||
| + | <th>Temperature</th> | ||
| + | <th>Time</th> | ||
| + | <th></th> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>94 ℃</td> | ||
| + | <td>3 min.</td> | ||
| + | <td rowspan="3">25～30 cycles</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>94 ℃</br>(Denaturation)</td> | ||
| + | <td>40 sec</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>57.5 ℃</br>(Annealing)</td> | ||
| + | <td>30 sec</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>72 ℃</br>(Extension)</td> | ||
| + | <td>Depend on sequence size</br>(2 kbp/min. for Taq)</td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>72 ℃</td> | ||
| + | <td>5 min.</td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>4 ℃</td> | ||
| + | <td>∞</td> | ||
| + | <td></td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | </div> | ||
</div> | </div> | ||
| Line 301: | Line 358: | ||
}); | }); | ||
</script> | </script> | ||
| + | <script src="https://2018.igem.org/Team:NCKU_Tainan/js/frame/T--NCKU_Tainan--jquery-1_12_4_min_js?action=raw&ctype=text/javascript"></script> | ||
| + | <script src="https://2018.igem.org/Template:NCKU_Tainan/js/bootstrap_min_js?action=raw&ctype=text/javascript"></script> | ||
| + | <script src="https://2018.igem.org/Template:NCKU_Tainan/js/model?action=raw&ctype=text/javascript"></script> | ||
| + | <script src='https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.5/MathJax.js?config=TeX-MML-AM_CHTML' async></script> | ||
</body> | </body> | ||
Revision as of 01:31, 21 September 2018