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With Warwick iGEM 2018 team we had several Skype sessions where we discussed about our projects, experiments and methods. | With Warwick iGEM 2018 team we had several Skype sessions where we discussed about our projects, experiments and methods. | ||
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They introduced us to websites such as benchling, where the FASTA(s) of desired gene(s) can be written and so the gene(s) are | They introduced us to websites such as benchling, where the FASTA(s) of desired gene(s) can be written and so the gene(s) are | ||
virtually inserted to a certain backbone (such as pSB1C3). The site enables the user to see any possible cut sites, which primers | virtually inserted to a certain backbone (such as pSB1C3). The site enables the user to see any possible cut sites, which primers | ||
can be and should be used and your gene circuit on the whole. | can be and should be used and your gene circuit on the whole. | ||
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The Warwick team, furthermore answered our questions via Instagram and provided help when we faced some problems with our protocols. | The Warwick team, furthermore answered our questions via Instagram and provided help when we faced some problems with our protocols. | ||
</p> | </p> | ||
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<p> | <p> | ||
With the attendance of all iGEM 2018 Teams from Turkey, we held a meet-up in our school's conference center on the 8th of September, 2018. | With the attendance of all iGEM 2018 Teams from Turkey, we held a meet-up in our school's conference center on the 8th of September, 2018. | ||
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Our goal in this meet-up was to get to know iGEM teams from Turkey, to gain helpful new insights, polish everyone's presentation skills, | Our goal in this meet-up was to get to know iGEM teams from Turkey, to gain helpful new insights, polish everyone's presentation skills, | ||
and to exchange advice, tips and thoughts. | and to exchange advice, tips and thoughts. | ||
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Since it's the first time that another high school iGEM Team from Turkey will be participating iGEM this year, we were especially enthusiastic | Since it's the first time that another high school iGEM Team from Turkey will be participating iGEM this year, we were especially enthusiastic | ||
to have Saint Joseph HS iGEM Team as a guest, and share our experiences with them. | to have Saint Joseph HS iGEM Team as a guest, and share our experiences with them. | ||
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We had the meet-up opening with the sincere music performance of students from Mavi Çizgi music school. After the music, Tuğba İnanç ,our PI, | We had the meet-up opening with the sincere music performance of students from Mavi Çizgi music school. After the music, Tuğba İnanç ,our PI, | ||
talked about her experiences in iGEM with previous teams and officially initiated presentation session. Her presentation was followed by | talked about her experiences in iGEM with previous teams and officially initiated presentation session. Her presentation was followed by | ||
Dr. Ceyhun Kayıhan's speech in which he talked about the history of Synthetic Biology and its impact on the society. | Dr. Ceyhun Kayıhan's speech in which he talked about the history of Synthetic Biology and its impact on the society. | ||
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Then, team presentations started and followed the order as Saint Joseph iGEM, Bilkent UNAMBG, METU_HS_Ankara, and finally Acıbadem İstanbul. | Then, team presentations started and followed the order as Saint Joseph iGEM, Bilkent UNAMBG, METU_HS_Ankara, and finally Acıbadem İstanbul. | ||
We all have asked questions to each other, gave advice and shared our memories from the summer we left behind. It was refreshing to see new | We all have asked questions to each other, gave advice and shared our memories from the summer we left behind. It was refreshing to see new | ||
interpretations and ways of doing things. Also it was motivating to see that we were not the only ones to face problems and spend efforts to | interpretations and ways of doing things. Also it was motivating to see that we were not the only ones to face problems and spend efforts to | ||
overcome them. | overcome them. | ||
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After the scientific presentations session, to have some fun and build stronger bonds as iGEMmers, together with Saint Joseph team, we followed | After the scientific presentations session, to have some fun and build stronger bonds as iGEMmers, together with Saint Joseph team, we followed | ||
the route to a tour in METU campus, which is known to be green, refreshing and fun. Along the way, we shared our hilarious memories and struggles | the route to a tour in METU campus, which is known to be green, refreshing and fun. Along the way, we shared our hilarious memories and struggles | ||
throughout our journey of iGEM. | throughout our journey of iGEM. | ||
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We all think that this meetup was productive, and we wish all the teams the best of luck. | We all think that this meetup was productive, and we wish all the teams the best of luck. | ||
</p> | </p> | ||
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As a highschool team, we were short on knowledge such as programming and modelling. Another setback was working through the summer, when many | As a highschool team, we were short on knowledge such as programming and modelling. Another setback was working through the summer, when many | ||
people were on vacation, but the iGEM teams. | people were on vacation, but the iGEM teams. | ||
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During the meetup presentations, one of the teams mentioned getting help and support from the iGEM Bielefeld CeBiTec 2018 team. They also | During the meetup presentations, one of the teams mentioned getting help and support from the iGEM Bielefeld CeBiTec 2018 team. They also | ||
mentioned being glad with how their project turned out. So we reached out to them. | mentioned being glad with how their project turned out. So we reached out to them. | ||
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Through our chats on Instagram, they acknowledged using multiple modeling approaches; they modeled toxicity kinetics of different metal ions | Through our chats on Instagram, they acknowledged using multiple modeling approaches; they modeled toxicity kinetics of different metal ions | ||
and promoters which they can use for metal uptake related parts and a filter system for water purification. Moreover, they also had a software | and promoters which they can use for metal uptake related parts and a filter system for water purification. Moreover, they also had a software | ||
tool for siRNA, which can be used for modeling. Unfortunately none of these modelling approaches suited our project in any aspect. However, | tool for siRNA, which can be used for modeling. Unfortunately none of these modelling approaches suited our project in any aspect. However, | ||
they wanted to help us improve our project, thus, they found us several programs that we could use to make a 3D model of an enzyme. | they wanted to help us improve our project, thus, they found us several programs that we could use to make a 3D model of an enzyme. | ||
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They sent us a list of tutorials and steps necessary to have a proper model. Additionally they taught us how to calculate the binding energy | They sent us a list of tutorials and steps necessary to have a proper model. Additionally they taught us how to calculate the binding energy | ||
between two proteins and then to load the proteins into the software tool; Chimera. Here we could modify them by removing helixes and use them | between two proteins and then to load the proteins into the software tool; Chimera. Here we could modify them by removing helixes and use them |
Revision as of 11:08, 21 September 2018