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− | <!doctype html>
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− | <html lang="en">
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− | <head>
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− | <meta charset="utf-8">
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− | <title>The HTML5 Herald</title>
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− | <meta name="description" content="The HTML5 Herald">
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− | <meta name="author" content="SitePoint">
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− | <link rel="stylesheet" href="https://2018.igem.org/Template:NCKU_Tainan/css/model?action=raw&ctype=text/css">
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− | </head>
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− | <table class="card card-body">
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− | <tr>
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− | <th>Plasmid</th>
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− | <th>200 ng</th>
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− | <th>1000 ng</th>
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− | </tr>
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− | <tr>
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− | <td>EcoRI / SpeI</td>
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− | <td>0.2 μl</td>
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− | <td>1 μl</td>
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− | </tr>
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− | <tr>
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− | <td>XbaI / PstI</td>
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− | <td>0.2 μl</td>
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− | <td>1 μl</td>
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− | </tr>
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− | <tr>
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− | <td>CutSmart Buffer</td>
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− | <td>2 μl</td>
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− | <td>5 μl</td>
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− | </tr>
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− | <tr>
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− | <td>ddH2O</td>
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− | <td>Up to 20 μl</td>
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− | <td>Up to 50 μl</td>
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− | </tr>
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− | <tr>
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− | <td>Digestion at 37℃ for 2.5hr.</td>
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− | <td></td>
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− | <td></td>
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− | </tr>
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− | </table>
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− |
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− | <table class="card card-body">
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− | <tr>
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− | <th>Plasmid</th>
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− | <th>200 ng</th>
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− | <th>1000 ng</th>
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− | </tr>
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− | <tr>
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− | <td>EcoRI / XbaI</td>
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− | <td>0.2 μl</td>
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− | <td>1 μl</td>
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− | </tr>
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− | <tr>
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− | <td>SpeI / PstI</td>
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− | <td>0.2 μl</td>
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− | <td>1 μl</td>
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− | </tr>
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− | <tr>
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− | <td>CutSmart Buffer</td>
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− | <td>2 μl</td>
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− | <td>5 μl</td>
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− | </tr>
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− | <tr>
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− | <td>ddH2O</td>
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− | <td>Up to 20 μl</td>
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− | <td>Up to 50 μl</td>
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− | </tr>
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− |
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− | <tr>
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− | <td>Digestion at 37℃ for 2.5hr.</td>
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− | <td></td>
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− | <td></td>
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− | </tr>
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− | </table>
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− | iGEMwiki toolssearchtocteamslogin
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− | Editing Team:NCKU Tainan/Protocol
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