Oscarliu117 (Talk | contribs) |
Oscarliu117 (Talk | contribs) |
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</ul></br> | </ul></br> | ||
+ | <h3>Construction of Pasr and Pgada</h3></br> | ||
+ | |||
+ | </br></br> | ||
+ | <p class="pcontent">5/9</br> </p> | ||
+ | <ul> | ||
+ | <li>Prepared the M9 medium in different pH value.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">5/11</br> </p> | ||
+ | <ul> | ||
+ | <li>Pre-cultured the DH5 alpha-pSB1C3 -Pasr-sfGFP strain.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">5/12</br> </p> | ||
+ | <ul> | ||
+ | <li>Cultured the DH5 alpha-pSB1C3 -Pasr-sfGFP strain in M9 medium in different pH value. | ||
+ | Measured the OD600 value and fluorescence every hour.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">5/18</br> </p> | ||
+ | <ul> | ||
+ | <li>Pre-cultured DH5 alpha-pSB1C3 –Pgada-RiboJ-sfGFP strain.</li> | ||
+ | <li>Prepared the M9 medium in different pH value.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">5/19</br> </p> | ||
+ | <ul> | ||
+ | <li>Cultured the DH5 alpha-pSB1C3 –Pgada-RiboJ-sfGFP strain in M9 medium in different pH | ||
+ | value. Measured the OD600 value and fluorescence every hour.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">6/2~6/3</br> </p> | ||
+ | <ul> | ||
+ | <li>Repeated the experiment on 5/11~5/12</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">7/29</br> </p> | ||
+ | <ul> | ||
+ | <li>Pre-culture the BL21(DE3)-pSB1C3 –Pasrr-sfGFP strain</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">7/30</br> </p> | ||
+ | <ul> | ||
+ | <li>Cultured the BL21(DE3)-pSB1C3 –Pasr -sfGFP strain on 96 well plate in different pH | ||
+ | value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">9/4</br> </p> | ||
+ | <ul> | ||
+ | <li>Pre-culture the BL21(DE3)-pSB1C3 –Pgada-RiboJ-sfGFP strain</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">9/5</br> </p> | ||
+ | <ul> | ||
+ | <li>Cultured the BL21(DE3)-pSB1C3 –Pgada-RiboJ-sfGFP strain strain on 96 well plate in | ||
+ | different pH value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | ||
+ | </ul></br> | ||
Revision as of 05:09, 22 September 2018