Difference between revisions of "Team:NCTU Formosa/Composite Part"

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           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This year we decided to create nine basic parts. These parts contain the single sequences of each antimicrobial peptide. Our goal is to assemble these basic parts with intein and chitin binding domain (CBD) sequences, which are better to purify. We listed all the basic parts we constructed below.
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           &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We tried to express the eight antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br>
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          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
 
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         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
 
         <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a>
 
         <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
 
         <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" >
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        <div class="title_1"><p>Improvement</p></div>
 
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Revision as of 14:44, 23 September 2018

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Overview

     We tried to express the eight antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

     The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

Antimicrobial Peptide

Subtilosin-Intein-CBD(BBa_K2599008)

Bovicin HJ50-Intein-CBD(BBa_K2599009)

Enterocin A-Intein-CBD(BBa_K2599010)

Enterocin B-Intein-CBD(BBa_K2599011)

Enterocin 96-Intein-CBD(BBa_K2599012)

Lacticin Z-Intein-CBD(BBa_K2599013)

Leucocyclicin Q-Intein-CBD(BBa_K2599014)

Durancin TW-49M-Intein-CBD(BBa_K2599015)

Improvement