Jenny860831 (Talk | contribs) |
Jenny860831 (Talk | contribs) |
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<p class="pcontent">4/26~4/27</br> </p> | <p class="pcontent">4/26~4/27</br> </p> | ||
<ul> | <ul> | ||
− | <li>PCR P<sub>asr</sub> and | + | <li>PCR P<sub>asr</sub> and P<sub>gadA</sub> from <i>E. coli</i> MG1655 chromosome. Confirmed and extracted |
products by DNA gel. </li> | products by DNA gel. </li> | ||
</ul></br> | </ul></br> | ||
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<p class="pcontent">4/28</br> </p> | <p class="pcontent">4/28</br> </p> | ||
<ul> | <ul> | ||
− | <li>Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with | + | <li>Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with P<sub>asr</sub> / P<sub>gadA</sub> and |
RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 | RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 | ||
alpha and spread the bacteria on LB plates.</li> | alpha and spread the bacteria on LB plates.</li> |
Revision as of 15:43, 5 October 2018