Jenny860831 (Talk | contribs) |
Jenny860831 (Talk | contribs) |
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<p class="pcontent">5/9</br> </p> | <p class="pcontent">5/9</br> </p> | ||
<ul> | <ul> | ||
− | <li>Store the correct colonies in glycerol in - | + | <li>Store the correct colonies in glycerol in -80°C.</li> |
</ul></br> | </ul></br> | ||
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<p class="pcontent">5/11</br> </p> | <p class="pcontent">5/11</br> </p> | ||
<ul> | <ul> | ||
− | <li>Pre-cultured the DH5 alpha-pSB1C3 - | + | <li>Pre-cultured the DH5 alpha-pSB1C3 -P<sub>asr</sub>-sfGFP strain.</li> |
</ul></br> | </ul></br> | ||
<p class="pcontent">5/12</br> </p> | <p class="pcontent">5/12</br> </p> | ||
<ul> | <ul> | ||
− | <li>Cultured the DH5 alpha-pSB1C3 - | + | <li>Cultured the DH5 alpha-pSB1C3 -P<sub>asr</sub>-sfGFP strain in M9 medium in different pH |
value. | value. | ||
− | Measured the | + | Measured the O.D. 600 value and fluorescence every hour.</li> |
</ul></br> | </ul></br> | ||
<p class="pcontent">5/18</br> </p> | <p class="pcontent">5/18</br> </p> | ||
<ul> | <ul> | ||
− | <li>Pre-cultured DH5 alpha-pSB1C3 | + | <li>Pre-cultured DH5 alpha-pSB1C3 –P<sub>gadA</sub>-RiboJ-sfGFP strain.</li> |
<li>Prepared the M9 medium in different pH value.</li> | <li>Prepared the M9 medium in different pH value.</li> | ||
</ul></br> | </ul></br> | ||
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<p class="pcontent">5/19</br> </p> | <p class="pcontent">5/19</br> </p> | ||
<ul> | <ul> | ||
− | <li>Cultured the DH5 alpha-pSB1C3 | + | <li>Cultured the DH5 alpha-pSB1C3 –P<sub>gadA</sub>-RiboJ-sfGFP strain in M9 medium in different |
pH | pH | ||
− | value. Measured the | + | value. Measured the O.D. 600 value and fluorescence every hour.</li> |
</ul></br> | </ul></br> | ||
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<p class="pcontent">7/29</br> </p> | <p class="pcontent">7/29</br> </p> | ||
<ul> | <ul> | ||
− | <li>Pre-culture the BL21(DE3)-pSB1C3 | + | <li>Pre-culture the BL21(DE3)-pSB1C3 –P<sub>asr</sub>-sfGFP strain</li> |
</ul></br> | </ul></br> | ||
<p class="pcontent">7/30</br> </p> | <p class="pcontent">7/30</br> </p> | ||
<ul> | <ul> | ||
− | <li>Cultured the BL21(DE3)-pSB1C3 | + | <li>Cultured the BL21(DE3)-pSB1C3 –P<sub>asr</sub> -sfGFP strain on 96 well plate in different pH |
value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | ||
</ul></br> | </ul></br> | ||
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<p class="pcontent">9/4</br> </p> | <p class="pcontent">9/4</br> </p> | ||
<ul> | <ul> | ||
− | <li>Pre-culture the BL21(DE3)-pSB1C3 | + | <li>Pre-culture the BL21(DE3)-pSB1C3 –P<sub>gadA</sub>-RiboJ-sfGFP strain</li> |
</ul></br> | </ul></br> | ||
<p class="pcontent">9/5</br> </p> | <p class="pcontent">9/5</br> </p> | ||
<ul> | <ul> | ||
− | <li>Cultured the BL21(DE3)-pSB1C3 | + | <li>Cultured the BL21(DE3)-pSB1C3 –P<sub>gadA</sub>-RiboJ-sfGFP strain strain on 96 well plate in |
different pH value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | different pH value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | ||
</ul></br> | </ul></br> | ||
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<p class="pcontent">7/12</br> </p> | <p class="pcontent">7/12</br> </p> | ||
<ul> | <ul> | ||
− | <li>Transformed the pSB1C3- | + | <li>Transformed the pSB1C3-P<sub>T7</sub>-CA into DH5 alpha. </li> |
</ul></br> | </ul></br> | ||
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<ul> | <ul> | ||
<li>Ligate the RbcL with PlacI.</li> | <li>Ligate the RbcL with PlacI.</li> | ||
− | <li>Transformed the pSB1C3- | + | <li>Transformed the pSB1C3-P<sub>T7</sub>-RbcL and pSB1C3-PlacI-RbcL into DH5 alpha.</li> |
</ul></br> | </ul></br> | ||
<p class="pcontent">8/3 </br> </p> | <p class="pcontent">8/3 </br> </p> | ||
<ul> | <ul> | ||
− | <li>Confirmed the pSB1C3- | + | <li>Confirmed the pSB1C3-P<sub>T7</sub>-RbcL construct by colony PCR.</li> |
<li>Sent the gene to sequencing</li> | <li>Sent the gene to sequencing</li> | ||
</ul></br> | </ul></br> |
Revision as of 16:05, 5 October 2018