We tried to express the eight antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
Difference between revisions of "Team:NCTU Formosa/Composite Part"
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| − | We tried to express the eight antimicrobial peptides in <i>E. coli< | + | We tried to express the eight antimicrobial peptides in <i>E. coli</i>. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.<br><br> |
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>. | The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit <i>Bacillus subtilis</i>. | ||
</p> | </p> | ||
Revision as of 13:46, 8 October 2018
Overview
Bacteriocins
Subtilosin-Intein-CBD(BBa_K2599008)
Bovicin HJ50-Intein-CBD(BBa_K2599009)
Enterocin A-Intein-CBD(BBa_K2599010)
Enterocin B-Intein-CBD(BBa_K2599011)
Enterocin 96-Intein-CBD(BBa_K2599012)
Lacticin Z-Intein-CBD(BBa_K2599013)
Leucocyclicin Q-Intein-CBD(BBa_K2599014)
Durancin TW-49M-Intein-CBD(BBa_K2599015)
Improvement
