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Revision as of 13:53, 10 October 2018
Collaboration
iGEM NTU Singapore
WHAT WE DO?
ModVision and Finding Diphthy has found their way to do collaboration in iGEM 2018. We have managed to contact one of the members of iGEM NTU via Angelysia Cardilla in the beginning of July. We offered help in gathering data about the idea and survey application of Nanopore sequencing from the perspective of Indonesian people as representatives of developing country’s citizens. Our team went to Faculty of Engineering, exactly located at college auditorium, to do intensive proposal towards nearby biotech students to be intrigued with our project about Finding Diphthy, as well as ModVision. QnA sessions and survey filling form were available for the end of each introductory explanation. This session was such a participative event between NTU and UI, since different citizens have vast range of opinion regarding biological engineering. Profound discussion in several aspects, such as socioeconomic condition, law, bioethics, etc., of ModVision and Finding Diphthy development could offer insights about their application in countries, especially developing ones.
Figure 1. Our team presents projects of Finding Diphthy and ModVision in collaboration within iGEM 2018 for silver medal criteria. Discussion and surveys were done by our policy and practice division member iGEM UI Ainun Rahmania and Glory Lamria.
WHAT WE ASK?
After two months of lab session starting from June, we encountered such unexpected bad events in doing PCR amplification of the HT-1 and HT-2 fragments. These fragments are meant to be ligated linearly to form single basic biobrick part of HB-EGF/Tar since IDTTM were not able to synthesize as one gBlocks. The cloning primer Fwd and Rev (personally synthesized primer) and for amplification fail to specifically anneal in the 5’ and 3’ of both fragments. The results of the PCR amplification are shown in following figure 2.
Figure 2. Gradient temperature PCR for both HT-1 and HT-2 fragments. Note that more than 2 bands could be found in HT-1 and HT-2 fragment. These amplified bands explain that the synthetically designed primers were not specific enough. HT-1 fragment size is 1105 bp, and HT-2 fragment size is 864 bp. The indicated arrows showed the expected amplified fragments.