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| | <!--- Basic Page Needs========================================================================= --> | | <!--- Basic Page Needs========================================================================= --> |
| − | <meta charset="utf-8"> | + | <meta charset="utf-8"/> |
| − | <title>Overview</title>
| + | <title></title> |
| − | <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.2, user-scalable=yes" />
| + | <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.2, user-scalable=yes" /> |
| − | <meta name="description" content="Wiki of Peking iGEM 2016" />
| + | <meta name="description" content="Wiki of Peking iGEM 2018" /> |
| − | <meta name="author" content="Li Jiamian & Wang Yuqing">
| + | <meta name="author" content="Peking iGEM"/> |
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| − | <!-- Fix Overwrite the original iGEM style=================================================== -->
| + | <!-- Fix Overwrite the original iGEM style=================================================== --> |
| − | <link href="https://2016.igem.org/Template:Peking/css/fix?action=raw&ctype=text/css" rel="stylesheet" />
| + | <link href="https://2018.igem.org/Template:Peking/css/fix?action=raw&ctype=text/css" rel="stylesheet" /> |
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| − | <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/priorstyle?action=raw&ctype=text/css">
| + | <link rel="stylesheet" href="https://2018.igem.org/Template:Peking/css/notebook_panel?action=raw&ctype=text/css"/> |
| − | <style>
| + | <style> |
| − | .home_img{
| + | body { background: #D2D8D8 url(https://static.igem.org/mediawiki/2018/7/78/T--Peking--images_bodyBackground.jpeg); background-attachment:fixed;} |
| − | padding: 10px;
| + | .texttitle{ |
| − | margin: 10px;
| + | color:#6495ED; |
| − | width: 420px;
| + | font-size: 38px; |
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| + | font-family: raleway-bold, sans-serif !important; |
| − | .home_text{
| + | background: transparent; |
| − | padding-top: 20px;
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| − | color: #11abb0;
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| − | font-size: 38px;
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| − | line-height: 48px;
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| − | margin-bottom: 12px;
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| − | text-align: center;
| + | figure{margin-top:40px;margin-bottom:40px;height:auto;} |
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| − | font-size:11px;
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| | + | <!--sidebar 引用==============================================================================--> |
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| | <!-- Navigation --> | | <!-- Navigation --> |
| | <div id="navigation" class="navbar navbar-fixed-top"> | | <div id="navigation" class="navbar navbar-fixed-top"> |
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| | </a> | | </a> |
| | <div id="logo" style="max-width:0px"><a class="" href="https://2018.igem.org/Team:Peking"><img | | <div id="logo" style="max-width:0px"><a class="" href="https://2018.igem.org/Team:Peking"><img |
| − | src="https://static.igem.org/mediawiki/2018/e/e2/T--Peking--_toolbar.jpeg" width="45%"></a></div> | + | src="https://static.igem.org/mediawiki/2018/3/36/T--Peking--Logo.png"></a></div> |
| | | | |
| | <div class="nav-collapse collapse"> | | <div class="nav-collapse collapse"> |
| − | <ul class="nav"> | + | <ul class="nav"> |
| − | <li class=" dropdown menu-1"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >              Project</a> | + | |
| | + | <li class="menu-1"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking">Home</a> |
| | + | </li> |
| | + | |
| | + | <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Project</a> |
| | <ul class="dropdown-menu"> | | <ul class="dropdown-menu"> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Project">Project</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking/Project" class="barfont1">Description</a></li> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Description" class="barfont1">Description</a></li>
| + | |
| | <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Design" class="barfont1">Design</a></li> |
| | <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Demonstrate" class="barfont1">Demonstration</a></li> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Future_Plan" class="barfont1">Future Plan</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking/Perspective" class="barfont1">Perspective</a></li> |
| | </ul> | | </ul> |
| | </li> | | </li> |
| − | <li class=" dropdown menu- 2"><a class=" dropdown- toggle" data-toggle="dropdown" href="#" >Data Page</a> | + | <li class="menu- 3"><a class=" colapse- menu1" href="https://2018.igem.org/Team:Peking/ Model"> Modeling</a> |
| − | <ul class="dropdown-menu">
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Results">Data Page</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Phase_Separation_D" class="barfont1">Phase Separation</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Functions_D" class="barfont1">Functions</a></li>
| + | |
| − | </ul>
| + | |
| | </li> | | </li> |
| − | <li class=" dropdown menu- 3"><a class=" dropdown- toggle" data-toggle="dropdown" href="#" >Model</a> | + | <li class="menu- 4"><a class=" colapse- menu1" href="https://2018.igem.org/Team:Peking/ Software"> Software</a> |
| − | <ul class="dropdown-menu">
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Model">Model</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Phase_Separation_M" class="barfont1">Phase Separation</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Functions_M" class="barfont1">Functions</a></li>
| + | |
| − | </ul>
| + | |
| | </li> | | </li> |
| − | <li class="menu- 4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software" >Software</a> | + | |
| − | </li>
| + | |
| − | <li class="dropdown menu-5"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Human Practices</a>
| + | <li class="menu- 6"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Human_Practices">Human Practices</a> |
| − | <ul class="dropdown-menu">
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Human_Practices/Gold" class="barfont1">Human Practices</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Public_Engagement" class="barfont1">Public Engagement</a></li>
| + | |
| − | </ul>
| + | |
| | </li> | | </li> |
| − | <li class="dropdown menu- 6"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Team</a> | + | <li class="dropdown menu- 7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievement</a> |
| | <ul class="dropdown-menu"> | | <ul class="dropdown-menu"> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Team_Members" class="barfont1">Team Members</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking/Judging_Form" class="barfont1">Judging Form</a></li> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Attributions" class="barfont1">Attributions</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking/Parts" class="barfont1">Parts</a></li> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking/Improve" class="barfont1">Improvement</a></li> |
| − | <li><a href="https://2018.igem.org/Team:Peking/Notebook" class="barfont1">Notebook</a></li> | + | <li><a href="https://2018.igem.org/Team:Peking/InterLab" class="barfont1">InterLab</a></li> |
| | + | |
| | </ul> | | </ul> |
| | </li> | | </li> |
| − | <li class="dropdown menu- 7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Collaborations</a> | + | <li class="dropdown menu- 8"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Lab</a> |
| | <ul class="dropdown-menu"> | | <ul class="dropdown-menu"> |
| | + | <li><a href="https://2018.igem.org/Team:Peking/Team_Members">Team Members</a></li> |
| | + | <li><a href="https://2018.igem.org/Team:Peking/Attributions" class="barfont1">Attributions</a></li> |
| | + | <li><a href="https://2018.igem.org/Team:Peking/Notebook" class="barfont1">Notebook</a></li> |
| | <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Collaborations" class="barfont1">Collaborations</a></li> |
| | + | <li><a href="https://2018.igem.org/Team:Peking/Safety" class="barfont1">Safety</a></li> |
| | <li><a href="https://2018.igem.org/Team:Peking/Acknowledgement" class="barfont1">Acknowledgement</a></li> | | <li><a href="https://2018.igem.org/Team:Peking/Acknowledgement" class="barfont1">Acknowledgement</a></li> |
| − | <li><a href="https://2018.igem.org/Team:Peking/InterLab" class="barfont1">InterLab</a></li> | + | |
| − | </ul>
| + | |
| − | </li>
| + | |
| − | <li class="dropdown menu-8"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Parts</a>
| + | |
| − | <ul class="dropdown-menu">
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Parts">Parts</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Favorite_Parts" class="barfont1">Favorite Parts</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Part_Table" class="barfont1">Part Table</a></li>
| + | |
| − | <li><a href="https://2018.igem.org/Team:Peking/Part_Collection" class="barfont1">Part Collection</a></li>
| + | |
| | </ul> | | </ul> |
| | </li> | | </li> |
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| − | | + | <!-- Page Title======================================================================== --> |
| − |
| + | <div id="page-title"> |
| − | | + | <div class="row"> |
| − | <div class="content-outer"> | + | <div class="twelve columns centered text-center"> |
| − | <div id="page-content" class="row page"> | + | <h1>Parts</h1> |
| − | | + | <p class="title1" style="text-align:center"></p> |
| − | | + | </div> |
| − | | + | </div> |
| − | 1231231234567
| + | </div> |
| − | | + | <!-- Page Title End--> |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </div> <!-- page-content End--> | + | |
| − | </div> <!-- Content End--> | + | |
| | | | |
| | | | |
| | + | <div id="page-content" class="row page"> |
| | + | <div id="primary" class="twelve columns"> |
| | + | <section> |
| | + | <div class="row"> |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | <div class="three columns"> |
| | + | <div id="page-wrap"> |
| | + | <div id="sidebar" style="color:#000000"> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('favor')">Favorite Parts</a></h4> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('table')">Part Table</a></h4> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('collection')">Part Collection</a></h4> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | |
| | + | |
| | + | <div class="nine columns"> |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | <div class="texttitle"><a id="favor"></a>Favorite Parts</div> |
| | + | <h3 class="classic-title">Schematic diagram</h3> |
| | + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| | + | <figure> |
| | + | <p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2018/d/df/T--Peking--parts0.png" alt=""/></p> |
| | + | <figcaption style="text-align:center;"> |
| | + | Figure. 1 Schematic diagram of most parts submitted by Peking iGEM 2018. |
| | + | </figcaption> |
| | + | </figure> |
| | + | <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K2601010">BBa_K2601010</a></h3> |
| | + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| | + | <figure> |
| | + | <p style="text-align:center;"><img src=" https://static.igem.org/mediawiki/2018/1/1f/T--Peking--parts1.png" alt=""/></p> |
| | + | <figcaption style="text-align:center;"> |
| | + | Figure. 2 Schematic diagram of BBa_K2601010 |
| | + | </figcaption> |
| | + | </figure> |
| | + | <p class="lead add-bottom" style="color:#5E5656">We combined Frb, yEGFP and HOTag6 togethor in <a href="http://parts.igem.org/Part:BBa_K2601010">BBa_K2601010</a>. Frb can interact with FKBP after adding rapamycin, which makes the RapaSPOT formation controllable. We constructed yeast strains with FKBP-mCherry-HOTag3 and Frb-yEGFP-HOTag6 and used 10 μM rapamycin to induce SPOT formation. Minutes after adding rapamycin, granules appeared and became larger gradually.</p> |
| | + | <br/> |
| | + | <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K2601011">BBa_K2601011</a></h3> |
| | + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| | + | <figure> |
| | + | <p style="text-align:center;"><div align="center"><img src=" https://static.igem.org/mediawiki/2018/8/87/T--Peking--parts2.jpeg" alt=""/></p></div> |
| | + | <figcaption style="text-align:center;"> |
| | + | Figure. 3 Schematic diagram of BBa_K2601011 |
| | + | </figcaption> |
| | + | </figure> |
| | + | <p class="lead add-bottom" style="color:#5E5656">To make our SPOT a reaction hub, we fused the enzymes of β-carotene synthase system, CrtI, CrtE and CrtYB into Frb-HOTag6 backbone. In the presence of rapamycin, FKBP-yEGFP-HOTag3 and Frb-enzyme-HOTag6 can condense into aggregates. And if the yeast contains SPOT, there will be more β-carotene producted. Meanwhile, we wondered if we can load enzymes onto SPOT mediated by indirected connection. We fused CFP to anti-GFP nanobody as the demonstration of our design and we observed the co-localization of the blue and green fluorescence as expected. </p> |
| | + | <br/> |
| | + | |
| | + | |
| | + | |
| | + | <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K2601012">BBa_K2601012</a></h3> |
| | + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| | + | <figure> |
| | + | <div align="center"><p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2018/3/36/T--Peking--parts3.png" alt=""/></p></div> |
| | + | <figcaption style="text-align:center;"> |
| | + | Figure. 4 Schematic diagram of BBa_K2601012. |
| | + | </figcaption> |
| | + | </figure> |
| | + | <p class="lead add-bottom" style="color:#5E5656">In the biobrick <a href="http://parts.igem.org/Part:BBa_K2601012">BBa_K2601012</a>, we fused SUMO, yEGFP and HOTag3 together. SUMO is the interaction module which can bind SIM spontaneously, while HOTag3 can introduce multivalence. These two features are necessary for SPOT formation. We expressed SUMO-yEGFP-HOTag3 and SIM-mCherry-HOTag6 in one yeast strain, and we observed the colocalization of yEGFP and mCherry in two different fluorescence channels, which confirms our hypothesis that the two components could form synthetic organelles. </p> |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | <br/> |
| | + | <a id="table"></a> |
| | + | <div class="texttitle">Part Table</div> |
| | + | |
| | + | <div class="highlight" align="center" > |
| | + | <table cellpadding="3" cellspacing="0" style="width: 60%;margin:auto"> |
| | + | <tr> |
| | + | <td width="30%">BBa_</td> |
| | + | <td align="right">Part</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601000">K2601000</a></td> |
| | + | <td align="right">Promoter-tet07</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601001">K2601001</a></td> |
| | + | <td align="right">Promoter-PDH3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601002">K2601002</a></td> |
| | + | <td align="right">SUMO</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601003">K2601003</a></td> |
| | + | <td align="right">SIM</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601004">K2601004</a></td> |
| | + | <td align="right">HOTag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601005">K2601005</a></td> |
| | + | <td align="right">HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601007">K2601007</a></td> |
| | + | <td align="right">Frb-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601008">K2601008</a></td> |
| | + | <td width="570%" align="right">FKBP-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601010">K2601010</a></td> |
| | + | <td align="right">Frb-yEGFP-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601011">K2601011</a></td> |
| | + | <td align="right">FKBP-yEGFP-HOTag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601012">K2601012</a></td> |
| | + | <td align="right">SUMO-yEGFP-HOtag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601021">K2601021</a></td> |
| | + | <td align="right">Tet07-Frb-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601023">K2601023</a></td> |
| | + | <td align="right">PDH3-Frb-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601025">K2601025</a></td> |
| | + | <td align="right">Tet07-FKBP-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601026">K2601026</a></td> |
| | + | <td align="right">TEF1-FKBP-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601027">K2601027</a></td> |
| | + | <td align="right">PDH3-FKBP-yEGFP</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601032">K2601032</a></td> |
| | + | <td align="right">Tet07-Frb-yEGFP-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601033">K2601033</a></td> |
| | + | <td align="right">TEF1-Frb-yEGFP-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601034">K2601034</a></td> |
| | + | <td align="right">PDH3-Frb-yEGFP-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601037">K2601037</a></td> |
| | + | <td align="right">TEF1-FKBP-yEGFP-HOTag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601038">K2601038</a></td> |
| | + | <td align="right">PDH3-FKBP-yEGFP-HOTag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601040">K2601040</a></td> |
| | + | <td align="right">Tet07-SUMO-yEGFP-HOtag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601041">K2601041</a></td> |
| | + | <td align="right">TEF1-SUMO-yEGFP-HOtag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601042">K2601042</a></td> |
| | + | <td align="right">PDH3-SUMO-yEGFP-HOtag3</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601054">K2601054</a></td> |
| | + | <td align="right">PDH3-Frb-crtE-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601056">K2601056</a></td> |
| | + | <td align="right">Tet07-Frb-crtYB-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601058">K2601058</a></td> |
| | + | <td align="right">PDH3-Frb-crtYB-HOTag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601060">K2601060</a></td> |
| | + | <td align="right">SIM-crtE-Hotag6</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td><a href="http://parts.igem.org/Part:BBa_K2601061">K2601061</a></td> |
| | + | <td align="right">SIM-crtYB-Hotag6</td> |
| | + | </tr> |
| | + | </table> |
| | + | </div> |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | <a id="collection"></a> |
| | + | <div class="texttitle">Part Collection</div> |
| | + | <p class="lead add-bottom" style="color:#5E5656">2018 Peking iGEM team members devoted themselves to constructing a part collection that can drive phase separation. We have not only submitted all of new basic HOTag parts, but also provided multiple composite parts combining HOTags with other protein-protein interaction modules and fluorescent reporters. The basic part, HOTag3, is a homo-oligomeric short peptide containing only 30 amino acids. It has high stoichiometry, forming hexamer spontaneously. The HOTag3, together with another tetrameric HOTag6, can robustly drive phase separation upon protein-protein interaction. Protein-protein interaction is achieved by our dimerization parts, including FKBP/Frb and SUMO/SIM. Functions of all the parts were thoroughly tested. Some thermodynamic and kinetic properties of the parts were characterized as well. We believe the HOTag is useful tool for other iGEM teams to investigate protein phase separation and design synthetic organelles.</p> |
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