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− | <p>We introduce a magic protein, anti-GFP nanobody, which is very small (only 13-kDa, 1.5nm 2.5nm) and high-affinity (0.59nM) camelid antibody to GFP<sup>[8]</sup>. So we can use its characteristic to improve our designs. We can fuse GFP to the C-terminus of interaction modules and to the N-terminus of HOTags, and fuse function modules to the C-terminus of anti-GFP nanobodies. Then, with the help of interaction between anti-GFP nanobodies and GFP, synthetic organelles will “welcome” function modules, expected functions can be realized. You may ask: How does anti-GFP nanobody improve the design? Firstly, it will not make the protein extremely large and will reduce the effect on the structure of function modules, which can ensure the quality of functions. Secondly, it can bring components not belonging to the original structure to synthetic organelles, which can enlarge the enrichment range of synthetic organelles. Thirdly, it is easy to regulate the expression of target proteins. So you can see, nanobodies may do better and give you a surprise!</p>
| + | <!-- <p>We introduce a magic protein, anti-GFP nanobody, which is very small (only 13-kDa, 1.5nm 2.5nm) and high-affinity (0.59nM) camelid antibody to GFP<sup>[8]</sup>. So we can use its characteristic to improve our designs. We can fuse GFP to the C-terminus of interaction modules and to the N-terminus of HOTags, and fuse function modules to the C-terminus of anti-GFP nanobodies. Then, with the help of interaction between anti-GFP nanobodies and GFP, synthetic organelles will “welcome” function modules, expected functions can be realized. You may ask: How does anti-GFP nanobody improve the design? Firstly, it will not make the protein extremely large and will reduce the effect on the structure of function modules, which can ensure the quality of functions. Secondly, it can bring components not belonging to the original structure to synthetic organelles, which can enlarge the enrichment range of synthetic organelles. Thirdly, it is easy to regulate the expression of target proteins. So you can see, nanobodies may do better and give you a surprise!</p> |
| <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/e5/T--Peking--project_design9.jpeg" width="400px" height="175 px" ></div> | | <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/e5/T--Peking--project_design9.jpeg" width="400px" height="175 px" ></div> |
| <figcaption style="text-align:center;"> | | <figcaption style="text-align:center;"> |
| Figure. 7 Interaction of anti-GFP nanobody and GFP | | Figure. 7 Interaction of anti-GFP nanobody and GFP |
| </figcaption> | | </figcaption> |
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− | <div class="texttitle">Conclusion | + | <div class="texttitle">Colony Forming Units per 0.1 OD600 E. coli cultures |
− | <a id="C"></a></div> | + | <a id="J"></a></div> |
| <hr style="border:2px dashed; height:2px" color="#666666"> | | <hr style="border:2px dashed; height:2px" color="#666666"> |
| <div class="coll"> | | <div class="coll"> |
| <div class="content"> | | <div class="content"> |
| + | <!-- |
| <p>We artificially designed phase separation in cells and synthesized membraneless organelles. And the main work to synthesize an organelle is to fulfill phase separation in a cell, so we stress the importance of interactions and multivalency. For these two aspects, we gave our ideas and the feasibility was analyzed. At last, we proposed two ideas to implement functions. We believe that in the near future, “millions of dollars” will no longer be a dream!</p> | | <p>We artificially designed phase separation in cells and synthesized membraneless organelles. And the main work to synthesize an organelle is to fulfill phase separation in a cell, so we stress the importance of interactions and multivalency. For these two aspects, we gave our ideas and the feasibility was analyzed. At last, we proposed two ideas to implement functions. We believe that in the near future, “millions of dollars” will no longer be a dream!</p> |
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| + | </div> |
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8c/T--Peking--CFU.png" ></div> |
| + | <figcaption style="text-align:center;"> |
| + | Figure. 13 The count of colony forming units per 0.1 OD600 E. coli cultures. |
| + | </figcaption> |
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| </div> | | </div> |
| </div> | | </div> |