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<td><a href="https://static.igem.org/mediawiki/2018/f/f9/T--Newcastle--MICCode.pdf" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/f/f9/T--Newcastle--MICCode.pdf" style="color:green">View</a></td> | ||
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+ | <td>2-Part Gibson Assembly</td> | ||
+ | <td>Frank Eardley</td> | ||
+ | <td>Endophyte Development</td> | ||
+ | <td><a href="https://static.igem.org/mediawiki/2018/7/78/T--Newcastle--2PartGibson.pdf" style="color:green">View</a></td> | ||
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Revision as of 10:34, 17 October 2018
Experiments
Protocols
EXPERIMENTS
PROTOCOLS
Protocol | Author | Category | PDF File |
---|---|---|---|
Glycerol Stock Preserves | Connor Trotter | General | View |
Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
Cell Counting | Connor Trotter | Chemotaxis | View |
Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
0.75% Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
Growth Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
Naringenin Kill Curve in Liquid Culture | Sadiya Quazi | Chemotaxis | View |
Planting Seeds in Agar-Filled Microcentrifuge Tubes | Lewis Tomlinson | Endophyte Development | View |
Preparation and Staining of Microscopy Samples | Lewis Tomlinson | Endophyte Development | View |
Planting Seeds in Agar-Filled Pipette-Tip Racks | Lewis Tomlinson | Endophyte Development | View |
Re-isolating Endophytes | Lewis Tomlinson | Endophyte Development | View |
Electroporation of Pseudomonas sp. | Frank Eardley | Endophyte Development | View |
MIC Opentrons Code (2 Plates) | Frank Eardley | Endophyte Development | View |
2-Part Gibson Assembly | Frank Eardley | Endophyte Development | View |
Amplification of gBlocks and Gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Colony PCR of Transformed DH5α Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
DpnI Digestion and Purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Gel Extraction and Purification of the gBlocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Gibson Assembly of gBlocks and pSB1C3 Backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Making Chemically Competent Cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Miniprep of Transformed Colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Amplification, Purification and Quantification of the pSB1C3 Backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Quantifying DNA Concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Resuspending gBlocks and Primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
SOC Media Preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
Transformation of the gBlocks into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |