Line 3: | Line 3: | ||
− | <canvas id="base_page" width="800" height="400" style=""> | + | |
− | + | <canvas id="base_page" width="800" height="400" style=""> | |
− | + | 您的浏览器不支持 HTML5 canvas 标签。 | |
− | + | </canvas> | |
− | + | <script> | |
− | + | var width = 800; | |
− | + | var height = 400; | |
− | var c = document.getElementById("base_page"); | + | |
− | + | var c = document.getElementById("base_page"); | |
− | $("#base_page").attr("width", c.parentElement.clientWidth); | + | |
− | + | $("#base_page").attr("width", c.parentElement.clientWidth); | |
− | var ctx = c.getContext("2d"); | + | |
− | + | var ctx = c.getContext("2d"); | |
− | + | that = ctx; | |
− | + | var i = 0; | |
− | + | var pi = Math.PI; | |
− | + | var basex = c.width / 2; | |
− | + | var basey = c.height / 2; | |
− | + | width = c.width; | |
− | + | height = c.height; | |
− | + | var imgx; | |
− | + | var imgy; | |
− | var w1 = "https://static.igem.org/mediawiki/2018/a/a8/T--NEU_China_B--w1.png"; | + | |
− | + | var w1 = "https://static.igem.org/mediawiki/2018/a/a8/T--NEU_China_B--w1.png"; | |
− | + | var w2 = "https://static.igem.org/mediawiki/2018/f/f9/T--NEU_China_B--w2.png"; | |
− | + | var w3 = "https://static.igem.org/mediawiki/2018/2/27/T--NEU_China_B--w3.png"; | |
− | + | var w4 = "https://static.igem.org/mediawiki/2018/9/96/T--NEU_China_B--w4.png"; | |
− | + | var w5 = "https://static.igem.org/mediawiki/2018/7/7a/T--NEU_China_B--w5.png"; | |
− | + | var w6 = "https://static.igem.org/mediawiki/2018/6/66/T--NEU_China_B--w6.png"; | |
− | var img1 = new Image(); | + | |
− | + | var img1 = new Image(); | |
− | + | var img2 = new Image(); | |
− | + | var img3 = new Image(); | |
− | + | var img4 = new Image(); | |
− | + | var img5 = new Image(); | |
− | + | var img6 = new Image(); | |
− | + | img1.src = w1; | |
− | + | var during = 40; | |
− | + | var t1 = 0; | |
− | + | var base_t1 = 0; | |
− | + | var t2 = 0; | |
− | + | var base_t2 = base_t1 + during; | |
− | + | var t3 = 0; | |
− | + | var base_t3 = base_t2 + during; | |
− | + | var t4 = 0; | |
− | + | var base_t4 = base_t3 + during; | |
− | + | var t5 = 0; | |
− | + | var base_t5 = base_t4 + during; | |
− | + | var t6 = 0; | |
− | + | var base_t6 = base_t5 + during; | |
− | var x_e = 300; | + | |
− | + | var x_e = 300; | |
− | + | var y_e = 150; | |
− | function redraw() { | + | |
− | + | function redraw() { | |
− | + | ctx.clearRect(0, 0, width, height); | |
− | + | ||
− | + | var i = 0; | |
− | + | ctx.beginPath(); | |
− | + | var x = x_e * Math.cos(0 * 180 * 2) + basex; | |
− | + | var y = y_e * Math.sin(0 * 180 * 4) + basey; | |
− | + | ctx.moveTo(x, y); | |
− | + | for (i = 0; i < 0.05; i += 0.00001) { | |
− | + | var x = x_e * Math.cos(i * 180 * 2) + basex; | |
− | + | var y = y_e * Math.sin(i * 180 * 4) + basey; | |
− | + | ctx.lineTo(x, y); | |
− | + | } | |
− | + | ctx.strokeStyle = '#FAF0E6'; | |
− | + | ctx.lineWidth = 3; | |
− | + | ctx.stroke(); | |
− | + | ctx.closePath(); | |
− | + | ||
− | + | } | |
− | function draw(x, y, r) { | + | |
− | + | function draw(x, y, r) { | |
− | + | ctx.beginPath(); | |
− | + | ctx.arc(x, y, r, 0, Math.PI * 2, true); | |
− | + | ctx.fillStyle = "red"; | |
− | + | ctx.fill(); | |
− | + | ctx.closePath(); | |
− | + | } | |
− | function drawImg(imgWarpper, x, y) { | + | |
− | + | function drawImg(imgWarpper, x, y) { | |
− | + | var img = imgWarpper.img; | |
− | + | var drawWidth = img.width / 4; | |
− | + | var drawHeight = img.height / 4; | |
− | + | var imx = x - drawWidth / 2; | |
− | + | var imy = y - drawHeight / 2 | |
− | + | imgWarpper.x = imx; | |
− | + | imgWarpper.y = imy; | |
− | + | ctx.drawImage(img, imx, imy, drawWidth, drawHeight); | |
− | + | } | |
− | var imgWarpper1 = {}; | + | |
− | + | var imgWarpper1 = {}; | |
− | + | var imgWarpper2 = {}; | |
− | + | var imgWarpper3 = {}; | |
− | + | var imgWarpper4 = {}; | |
− | + | var imgWarpper5 = {}; | |
− | + | var imgWarpper6 = {}; | |
− | imgWarpper1.img = img1; | + | |
− | + | imgWarpper1.img = img1; | |
− | + | imgWarpper2.img = img2; | |
− | + | imgWarpper3.img = img3; | |
− | + | imgWarpper4.img = img4; | |
− | + | imgWarpper5.img = img5; | |
− | + | imgWarpper6.img = img6; | |
− | img1.onload = function () { | + | |
− | + | img1.onload = function () { | |
− | + | img2.src = w2; | |
− | + | img2.onload = function () { | |
− | + | img3.src = w3; | |
− | + | img3.onload = function () { | |
− | + | img4.src = w4; | |
− | + | img4.onload = function () { | |
− | + | img5.src = w5; | |
− | + | img5.onload = function () { | |
− | + | console.log("finish"); | |
− | + | img6.src = w6; | |
− | + | img6.onload = function () { | |
− | + | var timer = setInterval(function () { | |
− | + | redraw(); | |
− | + | i += 0.00001; | |
− | + | ||
− | + | t1 = i + base_t1; | |
− | + | t2 = i + base_t2; | |
− | + | t3 = i + base_t3; | |
− | + | t4 = i + base_t4; | |
− | + | t5 = i + base_t5; | |
− | + | t6 = i + base_t6; | |
− | + | ||
− | + | var img1_x = x_e * Math.cos(t1 * 180 * 2) + basex; | |
− | + | var img1_y = y_e * Math.sin(t1 * 180 * 4) + basey; | |
− | + | ||
− | + | drawImg(imgWarpper1, img1_x, img1_y); | |
− | + | ||
− | + | var img2_x = x_e * Math.cos(t2 * 180 * 2) + basex; | |
− | + | var img2_y = y_e * Math.sin(t2 * 180 * 4) + basey; | |
− | + | imgWarpper2.x = img2_x; | |
− | + | imgWarpper2.y = img2_y; | |
− | + | // imgWarpper2.w = i | |
− | + | drawImg(imgWarpper2, img2_x, img2_y); | |
− | + | ||
− | + | ||
− | + | var img3_x = x_e * Math.cos(t3 * 180 * 2) + basex; | |
− | + | var img3_y = y_e * Math.sin(t3 * 180 * 4) + basey; | |
− | + | drawImg(imgWarpper3, img3_x, img3_y); | |
− | + | ||
− | + | var img4_x = x_e * Math.cos(t4 * 180 * 2) + basex; | |
− | + | var img4_y = y_e * Math.sin(t4 * 180 * 4) + basey; | |
− | + | drawImg(imgWarpper4, img4_x, img4_y); | |
− | + | ||
− | + | ||
− | + | var img5_x = x_e * Math.cos(t5 * 180 * 2) + basex; | |
− | + | var img5_y = y_e * Math.sin(t5 * 180 * 4) + basey; | |
− | + | drawImg(imgWarpper5, img5_x, img5_y); | |
− | + | ||
− | + | ||
− | + | var img6_x = x_e * Math.cos(t6 * 180 * 2) + basex; | |
− | + | var img6_y = y_e * Math.sin(t6 * 180 * 4) + basey; | |
− | + | drawImg(imgWarpper6, img6_x, img6_y); | |
− | + | // if (y > 400) { | |
− | + | // clearInterval(timer); | |
− | + | // } | |
− | + | }, 10); | |
+ | |||
+ | } | ||
+ | } | ||
} | } | ||
} | } | ||
} | } | ||
} | } | ||
− | + | ||
− | + | function contains(imgWarpper, x, y) { | |
− | + | var img = imgWarpper.img; | |
− | + | var rect = {}; | |
− | + | var drawWidth = img.width / 4; | |
− | + | var drawHeight = img.height / 4; | |
− | + | rect.x = imgWarpper.x; | |
− | + | rect.y = imgWarpper.y;; | |
− | + | rect.x2 = drawWidth + rect.x; | |
− | + | rect.y2 = drawHeight + rect.y; | |
− | + | if (rect.x < x) | |
− | + | if (rect.x2 > x) | |
− | + | if (rect.y < y) | |
− | + | if (rect.y2 > y) | |
− | + | return true; | |
− | + | return false; | |
− | + | } | |
− | + | ||
− | + | c.addEventListener('click', function (event) { | |
− | + | var x = event.clientX - c.getBoundingClientRect().left; | |
− | c.addEventListener('click', function (event) { | + | var y = event.clientY - c.getBoundingClientRect().top; |
− | + | // console.log(x, y); | |
− | + | if (contains(imgWarpper1, x, y)) { | |
− | + | console.log("img 1"); | |
− | + | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/background'; | |
− | + | } | |
− | + | else if (contains(imgWarpper2, x, y)) { | |
− | + | console.log("img 2"); | |
− | + | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Basic_Part'; | |
− | + | } | |
− | + | else if (contains(imgWarpper3, x, y)) { | |
− | + | console.log("img3"); | |
− | + | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Team'; | |
− | + | } | |
− | + | else if (contains(imgWarpper4, x, y)) { | |
− | + | console.log("img4"); | |
− | + | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/InterLab'; | |
− | + | } | |
− | + | else if (contains(imgWarpper5, x, y)) { | |
− | + | console.log("img5"); | |
− | + | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Human_Practices'; | |
− | + | } | |
− | + | else if(contains(imgWarpper6,x,y)) | |
− | + | { | |
− | + | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Human_Practices'; | |
− | + | } | |
− | + | }); | |
+ | |||
+ | </script> | ||
<hr> | <hr> |
Revision as of 04:37, 17 October 2018
Abstract
The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing.