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$${\{C_{CO_2}\ in\}\ -\ \{C_{CO_2}\ out\}\ ≥\ \{C_{biomass}\}\ -\ | $${\{C_{CO_2}\ in\}\ -\ \{C_{CO_2}\ out\}\ ≥\ \{C_{biomass}\}\ -\ | ||
\{C_{xylose}\}...(2)}$$ | \{C_{xylose}\}...(2)}$$ | ||
− | Let {C<sub>CO<sub>2</sub> net</sub>} = {C<sub>CO<sub>2</sub> in</sub>} - {C<sub>CO<sub>2</sub> out</sub>}, equation (2) further simplifies to | + | Let {C<sub>CO<sub>2</sub> net</sub>} = {C<sub>CO<sub>2</sub> in</sub>} - {C<sub>CO<sub>2</sub> out</sub>}, equation (2) further simplifies to |
− | + | $${\{C_{CO_2}\ net\}\ ≥\ \{C_{biomass}\}\ -\ \{C_{xylose}\}...(3)}$$ | |
− | + | If C<sub>waste</sub> is very small and negligible, | |
we can obtain the net amount of carbon dioxide fixed over time. If, on the contrary, | we can obtain the net amount of carbon dioxide fixed over time. If, on the contrary, | ||
C<sub>waste</sub> cannot be neglected, equation (3) allows us to estimate the minimum net amount of carbon dioxide fixed. | C<sub>waste</sub> cannot be neglected, equation (3) allows us to estimate the minimum net amount of carbon dioxide fixed. | ||
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The O.D. 600 of engineered <i>E. coli</i> is measured after a 12-hour cultivation and the result obtained is 0.4511 O.D. . | The O.D. 600 of engineered <i>E. coli</i> is measured after a 12-hour cultivation and the result obtained is 0.4511 O.D. . | ||
Yin Li et al. reported that dry cell weight (DCW) of <i>E. coli</i> is $${0.35g \over {1 L \cdot O.D. 600}}$$ , determined by experiment. | Yin Li et al. reported that dry cell weight (DCW) of <i>E. coli</i> is $${0.35g \over {1 L \cdot O.D. 600}}$$ , determined by experiment. | ||
− | <i>E. coli</i> biomass contains 48% of carbon by mass | + | <i>E. coli</i> biomass contains 48% of carbon by mass. |
− | + | $${\{C_{CO_2}\ net\}\ ≥\ \{C_{biomass}\}\ -\ \{C_{xylose}\}...(3)}$$ | |
− | + | </p> | |
<p class="pcontent">On the other hand, C<sub>xylose</sub> can be calculated by multiplying the amount of xylose consumed | <p class="pcontent">On the other hand, C<sub>xylose</sub> can be calculated by multiplying the amount of xylose consumed | ||
per unit volume of broth to the mass percentage of carbon in xylose. | per unit volume of broth to the mass percentage of carbon in xylose. |
Revision as of 21:08, 17 October 2018